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Efficacy of mechanical vibration in regulating mesenchymal stem cells gene expression

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Abstract

This study aimed at investigating the expression of osteoblast and chondrocyte-related genes in mesenchymal stem cells (MSCs), derived from rabbit adipose tissue, under mechanical vibration. The cells were placed securely on a vibrator’s platform and subjected to 300 Hz of sinusoidal vibration, with a maximum amplitude of 10 μm, for 45 min per day, and for 14 consequent days, in the absence of biochemical reagents. The negative control group was placed in the conventional culture medium with no mechanical loading. The expression of osteoblast and chondrocyte-related genes was investigated using real-time polymerase chain reaction (real-time PCR). In addition, F-actin fiber structure and alignment with the help of actin filament fluorescence staining were evaluated, and the level of metabolic activity of MSCs was determined by the methyl thiazolyl tetrazolium assay. The real-time PCR study showed a significant increase of bone gene expression in differentiated cells, compared with MSCs (P < 0.05). On the other hand, the level of chondrocyte gene expression was not remarkable. Applying mechanical vibration enhanced F-actin fiber structure and made them aligned in a specific direction. It was also found that during the differentiation process, the metabolic activity of the cells increased (P < 0.05). The results of this work are in agreement with the well-accepted fact that the MSCs, in the absence of growth factors, are sensitive to low-amplitude, high-frequency vibration. Outcomes of this work can be applied in cell therapy and tissue engineering, when regulation of stem cells is required.

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Acknowledgments

The authors would like to express their gratitude to the Amirkabir University of Technology and the Pasteur Institute of Iran for their kind assistance, and are also grateful to Mahdi Rajaei for his critical thoughts.

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Authors and Affiliations

  1. Faculty of Biomedical Engineering, Amirkabir University of Technology, P. O. Box 1591634311, Tehran, Iran

    Atiyeh Sadat Safavi & Gholamreza Rouhi

  2. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

    Nooshin Haghighipour

  3. Biotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, Tehran, Iran

    Fatemeh Bagheri

  4. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Mohamadreza Baghaban Eslaminejad & Frough Azam Sayahpour

Authors
  1. Atiyeh Sadat Safavi
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  2. Gholamreza Rouhi
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  3. Nooshin Haghighipour
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  4. Fatemeh Bagheri
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  5. Mohamadreza Baghaban Eslaminejad
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  6. Frough Azam Sayahpour
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Correspondence to Gholamreza Rouhi.

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Editor: Tetsuji Okamoto

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Safavi, A.S., Rouhi, G., Haghighipour, N. et al. Efficacy of mechanical vibration in regulating mesenchymal stem cells gene expression. In Vitro Cell.Dev.Biol.-Animal 55, 387–394 (2019). https://doi.org/10.1007/s11626-019-00340-9

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  • DOI: https://doi.org/10.1007/s11626-019-00340-9

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