Abstract
Cardiomyocytes apoptosis following reperfusion injury causes irreversible damage to cardiac function. Understanding the mechanisms underlying cardiomyocytes death under these conditions can be helpful to identify strategies to abrogate such detrimental effects. Stem cell-specific proteins and regulatory pathways become important in understanding reparative processes in the myocardium. One such regulatory protein named nucleostemin (NS) has vital roles in cardiac ischemia. Although the relationship between NS and cell apoptosis has been studied, it is unknown how NS is controlled and how it participates in cardiomyocytes apoptosis induced by ischemia reperfusion (I/R). In the present study, we aimed to investigate the direct role of NS in myocardial I/R. In vivo, NS was highly expressed in cardiac tissues after I/R. Double immunofluorescent staining showed that NS located in the nucleolar of cardiomyocytes and correlated with cardiomyocytes apoptosis. Furthermore, in vitro primary rat cardiomyocytes increased NS expression induced by hypoxia-reoxygenation (H/R) treatment, in line with results in vivo. Suppression of NS expression by siNS promoted the expression of terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL)-positive cells p53 and cleaved caspase-3, which demonstrates I/R may require increased expression of NS to suppress p53 activation and maintain cardiomyocytes survival.
Similar content being viewed by others
References
Abbate A, Biondi-Zoccai GG, Baldi A (2002) Pathophysiologic role of myocardial apoptosis in post-infarction left ventricular remodeling. J Cell Physiol 193(2):145–153
Beekman C, Nichane M, De Clercq S, Maetens M, Floss T, Wurst W, Bellefroid E, Marine JC (2006) Evolutionarily conserved role of nucleostemin: controlling proliferation of stem/progenitor cells during early vertebrate development. Mol Cell Biol 26(24):9291–9301
Bolli R, Becker L, Gross G, Mentzer R Jr, Balshaw D, Lathrop DA, Ischemia NWGotToTfPtHf (2004) Myocardial protection at a crossroads: the need for translation into clinical therapy. Circ Res 95(2):125–134
Braunwald E, Kloner RA (1985) Myocardial reperfusion: a double-edged sword? J Clin Invest 76(5):1713–1719
Budhram-Mahadeo V, Fujita R, Bitsi S, Sicard P, Heads R (2014) Co-expression of POU4F2/Brn-3b with p53 may be important for controlling expression of pro-apoptotic genes in cardiomyocytes following ischaemic/hypoxic insults. Cell Death Dis 5, e1503
Fan Y, Liu Z, Zhao S, Lou F, Nilsson S, Ekman P, Xu D, Fang X (2006) Nucleostemin mRNA is expressed in both normal and malignant renal tissues. Br J Cancer 94(11):1658–1662
Forini F, Kusmic C, Nicolini G, Mariani L, Zucchi R, Matteucci M, Iervasi G, Pitto L (2014) Triiodothyronine prevents cardiac ischemia/reperfusion mitochondrial impairment and cell loss by regulating miR30a/p53 axis. Endocrinology 155(11):4581–4590
Gude NA, Emmanuel G, Wu W, Cottage CT, Fischer K, Quijada P, Muraski JA, Alvarez R, Rubio M, Schaefer E, Sussman MA (2008) Activation of Notch-mediated protective signaling in the myocardium. Circ Res 102(9):1025–1035
Guo Y, Liao YP, Zhang D, Xu LS, Li N, Guan WJ, Liu CQ (2014) In vitro study of nucleostemin as a potential therapeutic target in human breast carcinoma SKBR-3 cells. Asian Pac J Cancer Prev 15(5):2291–2295
Hariharan N, Quijada P, Mohsin S, Joyo A, Samse K, Monsanto M, De La Torre A, Avitabile D, Ormachea L, McGregor MJ, Tsai EJ, Sussman MA (2015) Nucleostemin rejuvenates cardiac progenitor cells and antagonizes myocardial aging. J Am Coll Cardiol 65(2):133–147
Hsieh PC, Segers VF, Davis ME, MacGillivray C, Gannon J, Molkentin JD, Robbins J, Lee RT (2007) Evidence from a genetic fate-mapping study that stem cells refresh adult mammalian cardiomyocytes after injury. Nat Med 13(8):970–974
Kajstura J, Cheng W, Reiss K, Clark WA, Sonnenblick EH, Krajewski S, Reed JC, Olivetti G, Anversa P (1996) Apoptotic and necrotic myocyte cell deaths are independent contributing variables of infarct size in rats. Lab Invest 74(1):86–107
Liu RL, Zhang ZH, Zhao WM, Wang M, Qi SY, Li J, Zhang Y, Li SZ, Xu Y (2008) Expression of nucleostemin in prostate cancer and its effect on the proliferation of PC-3 cells. Chin Med J (Engl) 121(4):299–304
Liu L, Zhang G, Liang Z, Liu X, Li T, Fan J, Bai J, Wang Y (2014) MicroRNA-15b enhances hypoxia/reoxygenation-induced apoptosis of cardiomyocytes via a mitochondrial apoptotic pathway. Apoptosis 19(1):19–29
Long X, Boluyt MO, Hipolito ML, Lundberg MS, Zheng JS, O'Neill L, Cirielli C, Lakatta EG, Crow MT (1997) p53 and the hypoxia-induced apoptosis of cultured neonatal rat cardiac myocytes. J Clin Invest 99(11):2635–2643
Maulik N, Engelman RM, Rousou JA, Flack JE 3rd, Deaton D, Das DK (1999) Ischemic preconditioning reduces apoptosis by upregulating anti-death gene Bcl-2. Circulation 100(19 Suppl):II369–375
Meng L, Lin T, Peng G, Hsu JK, Lee S, Lin SY, Tsai RY (2013) Nucleostemin deletion reveals an essential mechanism that maintains the genomic stability of stem and progenitor cells. Proc Natl Acad Sci U S A 110(28):11415–11420
Minamino T (2012) Cardioprotection from ischemia/reperfusion injury: basic and translational research. Circ J 76(5):1074–1082
Nomura J, Maruyama M, Katano M, Kato H, Zhang J, Masui S, Mizuno Y, Okazaki Y, Nishimoto M, Okuda A (2009) Differential requirement for nucleostemin in embryonic stem cell and neural stem cell viability. Stem Cells 27(5):1066–1076
Olivetti G, Quaini F, Sala R, Lagrasta C, Corradi D, Bonacina E, Gambert SR, Cigola E, Anversa P (1996) Acute myocardial infarction in humans is associated with activation of programmed myocyte cell death in the surviving portion of the heart. J Mol Cell Cardiol 28(9):2005–2016
Senturk T, Cavun S, Avci B, Yermezler A, Serdar Z, Savci V (2014) Effective inhibition of cardiomyocyte apoptosis through the combination of trimetazidine and N-acetylcysteine in a rat model of myocardial ischemia and reperfusion injury. Atherosclerosis 237(2):760–766
Siddiqi S, Gude N, Hosoda T, Muraski J, Rubio M, Emmanuel G, Fransioli J, Vitale S, Parolin C, D'Amario D, Schaefer E, Kajstura J, Leri A, Anversa P, Sussman MA (2008) Myocardial induction of nucleostemin in response to postnatal growth and pathological challenge. Circ Res 103(1):89–97
Sun J, Sun G, Meng X, Wang H, Wang M, Qin M, Ma B, Luo Y, Yu Y, Chen R, Ai Q, Sun X (2013a) Ginsenoside RK3 prevents hypoxia-reoxygenation induced apoptosis in H9c2 cardiomyocytes via AKT and MAPK pathway. Evid Based Complement Alternat Med 2013:690190
Sun Y, Yi W, Yuan Y, Lau WB, Yi D, Wang X, Wang Y, Su H, Wang X, Gao E, Koch WJ, Ma XL (2013b) C1q/tumor necrosis factor-related protein-9, a novel adipocyte-derived cytokine, attenuates adverse remodeling in the ischemic mouse heart via protein kinase A activation. Circulation 128(11 Suppl 1):S113–120
Tjwa M, Dimmeler S (2008) A nucleolar weapon in our fight for regenerating adult hearts: nucleostemin and cardiac stem cells. Circ Res 103(1):4–6
Tsai RY, McKay RD (2002) A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells. Genes Dev 16(23):2991–3003
Xie Z, Koyama T, Abe K, Fuji Y, Sawa H, Nagtashima K (2000) Upregulation of P53 protein in rat heart subjected to a transient occlusion of the coronary artery followed by reperfusion. Jpn J Physiol 50(1):159–162
Yellon DM, Hausenloy DJ (2007) Myocardial reperfusion injury. N Engl J Med 357(11):1121–1135
Yoshida R, Nakayama H, Nagata M, Hirosue A, Tanaka T, Kawahara K, Nakagawa Y, Matsuoka Y, Sakata J, Arita H, Hiraki A, Shinohara M, Ito T (2014) Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma. Br J Cancer 111(12):2308–2315
Zhou XL, Wan L, Xu QR, Zhao Y, Liu JC (2013) Notch signaling activation contributes to cardioprotection provided by ischemic preconditioning and postconditioning. J Transl Med 11:251
Acknowledgments
This work was supported in part by the National Natural Science Foundation of China (Nos. 81401365, 81373223, 81200918, 81172879), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Nantong University graduate scientific and technological innovation projects (No. YKS14010).
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Additional information
Editor: Tetsu Okamoto
Chi Zhang, Jiahai Shi, Xiang Wu and Xiaojuan Liu contributed equally to this work.
Rights and permissions
About this article
Cite this article
Zhang, C., Shi, J., Qian, L. et al. Nucleostemin exerts anti-apoptotic function via p53 signaling pathway in cardiomyocytes. In Vitro Cell.Dev.Biol.-Animal 51, 1064–1071 (2015). https://doi.org/10.1007/s11626-015-9934-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11626-015-9934-7