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Rhynchophorus ferrugineus midgut cell line to evaluate insecticidal potency of different plant essential oils

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Abstract

Cell cultures can be a potent and strong tool to evaluate the insecticidal efficiency of natural products. Plant essential oils have long been used as the fragrance or curative products around the world which means that they are safer to be used in close proximity of humans and mammals. In this study, a midgut cell line, developed from Rhynchophorus ferrugineus (RPW-1), was used for screening essential oils from nine different plants. Assays revealed that higher cell mortality was observed at 500 ppm which reached to 86, 65, 60, 59, 56, 54, 54, 53, and 53%, whereas lowest cell mortality at 1 ppm remained at 41, 23, 20, 17, 16, 15, 14, 13, and 10%, for Azadirachta indica, Piper nigrum, Mentha spicata, Cammiphora myrrha, Elettaria cardamomum, Zingiber officinale, Curcuma longa, Schinus molle, and Rosmarinus officinalis, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay revealed the percentage of cell growth inhibition was highest at 500 ppm and remained at 48, 45, 42, 37, 34, 29, 24, 22, and 18% against A. indica, P. nigrum, M. spicata, C. myrrha, E. cardamomum, Z. officinale, C. longa, S. molle, and R. officinalis, respectively. Lowest LC50 value (7.98 ppm) was found for A. indica, whereas the highest LC50 (483.11 ppm) was against R. officinalis. Thus, in this study, essential oils of A. indica exhibited the highest levels of toxicity, whereas those from R. officinalis exhibited the lowest levels of toxicity toward RPW-1 cells.

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Acknowledgment

The authors gratefully acknowledge the role of King Abdul Aziz City of Science and Technology (KACST) for providing research funding under the grant no. 08-BIO 10–6 to conduct this study.

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Authors and Affiliations

  1. Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Hofuf, Saudi Arabia

    Muhammad Rizwan-ul-Haq & Ahmed Mohammed Aljabr

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  1. Muhammad Rizwan-ul-Haq
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  2. Ahmed Mohammed Aljabr
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Correspondence to Ahmed Mohammed Aljabr.

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Editor: T. Okamoto

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Rizwan-ul-Haq, M., Aljabr, A.M. Rhynchophorus ferrugineus midgut cell line to evaluate insecticidal potency of different plant essential oils. In Vitro Cell.Dev.Biol.-Animal 51, 281–286 (2015). https://doi.org/10.1007/s11626-014-9825-3

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  • DOI: https://doi.org/10.1007/s11626-014-9825-3

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