Skip to main content
SpringerLink
Log in

Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation, Migration, Invasion and Angiogenesis of Adipose Derived Stem Cells

  • Published:
Current Medical Science Aims and scope Submit manuscript

Abstract

Preeclampsia (PE) is a pregnancy-specific hypertensive complication, closely related to endothelial dysfunction. Adipose derived stem cells (ADSCs) have the capacity to differentiate into endothelial cells for vascular repair. Therefore, we hypothesized that induced endothelial differentiation of ADSCs might hold great potential for the treatment of PE. In this study, the primary ADSCs and human umbilical vein endothelial cells (HUVECs) were isolated by the collagenase digestion method. The supernatant of HUVECs was collected from the first generation of cells. Then, ADSCs were divided into two groups: ADSCs alone group and induced ADSCs (iADSCs) group. In iADSCs group, ADSCs were induced by HUVECs conditioned medium and ADSCs special culture medium at a ratio of 1:1 over a two-week period. In order to identify the endothelial characteristics of iADSCs, CD31 and CD34 were examined by flow cytometry. The proliferation, migration, invasion and angiogenesis assays were employed to compare the bioactivity of iADSCs and ADSCs. Furthermore, The levels of angiogenic related factors including vascular endothelial growth factor (VEGF) and placenta growth factor (P1GF) were detected by RT-PCR and Western blotting. Results showed conditioned medium from HUVECs promoted ADSCs proliferation, migration, invasion and angiogenesis. In addition, the levels of VEGF and P1GF were significantly enhanced in iADSCs group. This study uncovered the iADSCs application potential in the therapy and intervention of PE.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. O'Gorman N, Wright D, Syngelaki A, etal. Competing risks model in screening for preeclampsia by maternal factors and biomarkers at 11–13 weeks gestation. Am J Obstet Gynecol, 2016,214(1): 103.el–103.el2

    Google Scholar 

  2. Cui K, Yan T, Luo Q, etal. Ultrasound microbubble-mediated delivery of integrin-linked kinase gene improves endothelial progenitor cells dysfunction in pre-eclampsia. DNA Cell Biol, 2014, 33(5): 301–310

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  3. Liu X, Luo Q, Zheng Y, etal. The role of Delta-like 4 ligand/Notch-ephrin-B2 cascade in the pathogenesis of preeclampsia by regulating functions of endothelial progenitor cell. Placenta, 2015, 36(9): 1002–1010

    Article  PubMed  CAS  Google Scholar 

  4. Zhu J, Cheng X, Wang Q, etal. Transplantation of endothelial progenitor cells for improving placental perfusion in preeclamptic rats. Arch Gynecol Obstet, 2015, 291(5): 1113–1119

    Article  PubMed  CAS  Google Scholar 

  5. Park SJ, Kim KJ, Kim WU, etal. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via Cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor. J Immunol, 2014, 192(7): 3003–3010

    Article  PubMed  CAS  Google Scholar 

  6. Chen CY, Liu SH, Chen CY, etal. Human placenta-derived multipotent mesenchymal stromal cells involved in placental angiogenesis via the PDGF-BB and STAT3 pathways. Biol Reprod, 2015,93(4): 103

    Article  PubMed  CAS  Google Scholar 

  7. Zhao G, Zhou X, Chen S, etal. Differential expression of microRNAs in decidua-derived mesenchymal stem cells from patients with pre-eclampsia. J BiomedSci, 2014,21:81

    Google Scholar 

  8. Du G, Liu Y, Dang M, etal. Comparison of administration routes for adipose-derived stem cells in the treatment of middle cerebral artery occlusion in rats. Acta Histochem, 2014, 116(6): 1075–1084

    Article  PubMed  Google Scholar 

  9. Diaz-Herraez P, Garbayo E, Simon-Yarza T, etal. Adipose-derived stem cells combined with neuregulin-1 delivery systems for heart tissue engineering. Eur J Pharm Biopharm, 2013, 85(1): 143–150

    Article  PubMed  CAS  Google Scholar 

  10. Mehrabani M, Najafi M, Kamarul T, etal. Deferoxamine preconditioning to restore impaired HIF-lalpha-mediated angiogenic mechanisms in adipose-derived stem cells from STZ-induced type 1 diabetic rats. Cell Prolif, 2015, 48(5): 532–549

    Article  PubMed  CAS  Google Scholar 

  11. Wang K, Yu LY, Jiang LY, etal. The paracrine effects of adipose-derived stem cells on neovascularization and biocompatibility of a macroencapsulation device. Acta Biomater, 2015, 15:65–76

    Article  PubMed  CAS  Google Scholar 

  12. Kishimoto S, Inoue K, Nakamura S, etal. Low-molecular weight heparin protamine complex augmented the potential of adipose-derived stromal cells to ameliorate limb ischemia. Atherosclerosis, 2016, 249:132–139

    Article  PubMed  CAS  Google Scholar 

  13. Chen X, Yan L, Guo Z, etal. Adipose-derived mesenchymal stem cells promote the survival of fat grafts via crosstalk between the Nrf2 and TLR4 pathways. Cell Death Dis, 2016,7(9): e2369

    Google Scholar 

  14. Chen J, Gu Z, Wu M, etal. C-reactive protein can upregulate VEGF expression to promote ADSC-induced angiogenesis by activating HIF-1 alpha via CD64/PI3k/Akt and MAPK/ERK signaling pathways. Stem Cell Res Ther, 2016,7(1): 114

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  15. Arana M, Pena E, Abizanda G, etal. Preparation and characterization of collagen-based ADSC-carrier sheets for cardiovascular application. Acta Biomater, 2013, 9(4): 6075–6083

    Article  PubMed  CAS  Google Scholar 

  16. Yue Y, Zhang P, Liu D, etal. Hypoxia preconditioning enhances the viability of ADSCs to increase the survival rate of ischemic skin flaps in rats. Aesthetic Plast Surg, 2013, 37(1): 159–170

    Article  PubMed  Google Scholar 

  17. Ding D, Mao D, Li K, etal. Precise and long-term tracking of adipose-derived stem cells and their regenerative capacity via superb bright and stable organic nanodots. ACS Nano, 2014,8(12): 12 620–12 631

    Article  CAS  Google Scholar 

  18. Rada T, Santos TC, Marques AP, etal. Osteogenic differentiation of two distinct subpopulations of human adipose-derived stem cells: an in vitro and in vivo study. J Tissue Eng Regen Med, 2012, 6(1): 1–11

    Article  PubMed  CAS  Google Scholar 

  19. Zhao Y, Zheng YF, Luo QQ, etal. Edaravone inhibits hypoxia-induced trophoblast-soluble Fms-like tyrosine kinase 1 expression: a possible therapeutic approach to preeclampsia. Placenta, 2014, 35(7): 476–482

    Article  PubMed  CAS  Google Scholar 

  20. Ruszkowska-Ciastek B, Sokup A, Leszcz M, etal. The number of circulating endothelial progenitor cells in healthy individuals-effect of some anthropometric and environmental factors (a pilot study). Adv Med Sei, 2015,60(1): 58–63

    Article  Google Scholar 

  21. Zhao X, Liu L, Wang FK, etal. Coculture of vascular endothelial cells and adipose-derived stem cells as a source for bone engineering. Ann Plast Surg, 2012,69(1): 91–98

    Article  PubMed  CAS  Google Scholar 

  22. Kim JH, Choi SC, Park CY, etal. Transplantation of Immortalized CD34+ and CD34-adipose-derived stem cells improve cardiac function and mitigate systemic pro-Inflammatory responses. PLoS One, 2016,11(2): e0147853

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  23. Colazzo F, Chester AH, Taylor PM, etal. Induction of mesenchymal to endothelial transformation of adipose-derived stem cells. J Heart Valve Dis, 2010, 19(6): 736–744

    PubMed  Google Scholar 

  24. Shevchenko EK, Makarevich PI, Tsokolaeva ZI, etal. Transplantation of modified human adipose derived stromal cells expressing VEGF165 results in more efficient angiogenic response in ischemic skeletal muscle. J Transl Med, 2013,11:138

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  25. Deveza L, Choi J, Imanbayev G, etal. Paracrine release from nonviral engineered adipose-derived stem cells promotes endothelial cell survival and migration in vitro. Stem Cells Dev, 2013, 22(3): 483–491

    Article  PubMed  CAS  Google Scholar 

  26. Colazzo F, Alrashed F, Saratchandra P, etal. Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells. Growth Factors, 2014, 32(5): 139–149

    Article  PubMed  CAS  Google Scholar 

  27. Zhang B, Yang S, Zhang Y, etal. Co-culture of mesenchymal stem cells with umbilical vein endothelial cells under hypoxic condition. J Huazhong Univ Sei Technolog Med Sei, 2012, 32(2): 173–180

    Article  Google Scholar 

  28. Li Q, Li PH, Hou DJ, etal. EGF enhances ADSCs secretion via ERK and JNK pathways. Cell Biochem Biophys, 2014, 69(1): 189–196

    Article  PubMed  CAS  Google Scholar 

  29. Aoki T, Suzuki Y, Nishio K, etal Effect of antioxidants on hyperoxia-induced ICAM-1 expression in human endothelial cells. Adv Exp Med Biol, 1997, 411:503–511

    Article  PubMed  CAS  Google Scholar 

  30. Kim J, Lee JH, Yeo SM, etal. Stem cell recruitment factors secreted from cord blood-derived stem cells that are not secreted from mature endothelial cells enhance wound healing. In Vitro Cell Dev Biol Anim, 2014, 50(2): 146–154

    Article  PubMed  CAS  Google Scholar 

  31. Arslan F, Lai RC, Smeets MB, etal. Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury. Stem Cell Res, 2013, 10(3): 301–312

    Article  PubMed  CAS  Google Scholar 

  32. Qin J, Yuan F, Peng Z, etal. Periostin enhances adipose-derived stem cell adhesion, migration, and therapeutic efficiency in Apo E deficient mice with hind limb ischemia. Stem Cell Res Ther, 2015,6:138

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  33. Lai WW, Hsu SC, Chueh FS, etal. Quercetin inhibits migration and invasion of SAS human oral cancer cells through inhibition of NF-kappaB and matrix metalloproteinase-2/-9 signaling pathways. Anticancer Res, 2013, 33(5): 1941–1950

    PubMed  CAS  Google Scholar 

  34. Shi Z, Neoh KG, Kang ET, etal. Enhanced endothelial differentiation of adipose-derived stem cells by substrate nanotopography. J Tissue Eng Regen Med, 2014,8(1): 50–58

    Article  PubMed  CAS  Google Scholar 

  35. Deng K, Lin DL, Hanzlicek B, etal. Mesenchymal stem cells and their secretome partially restore nerve and urethral function in a dual muscle and nerve injury stress urinary incontinence model. Am J Physiol Renal Physiol, 2015,308(2): F92–F100

    Article  PubMed  CAS  Google Scholar 

  36. Deng M, Gu Y, Liu Z, etal. Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh. Stem Cells Int, 2015,2015:350 718

    Article  CAS  Google Scholar 

  37. Efimenko A, Dzhoyashvili N, Kalinina N, etal. Adipose-derived mesenchymal stromal cells from aged patients with coronary artery disease keep mesenchymal stromal cell properties but exhibit characteristics of aging and have impaired angiogenic potential. Stem Cells Transl Med, 2014, 3(1): 32–41

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Ming-lian Luo  (骆名恋), Xiao-ping Liu  (刘小平), Fang Wang  (王 芳), Xiao-xia Liu  (刘晓夏), Wei-fang Liu  (刘维芳), Di Wu  (吴 迪), Hui Tao  (陶 惠), Rong-li Wang  (王绒莉), Yin Zhao  (赵 茵), Jian-wen Zhu  (朱剑文) & Li Zou  (邹 丽)

  2. Department of Obstetrics and Gynecology, Wuhan First Hospital, Wuhan, 430022, China

    Ming-lian Luo  (骆名恋)

Authors
  1. Ming-lian Luo  (骆名恋)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-ping Liu  (刘小平)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fang Wang  (王 芳)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiao-xia Liu  (刘晓夏)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wei-fang Liu  (刘维芳)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Di Wu  (吴 迪)
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hui Tao  (陶 惠)
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Rong-li Wang  (王绒莉)
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yin Zhao  (赵 茵)
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jian-wen Zhu  (朱剑文)
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Li Zou  (邹 丽)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jian-wen Zhu  (朱剑文) or Li Zou  (邹 丽).

Additional information

This project was supported by National Natural Science Foundation of China (No. 81100428).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Luo, Ml., Liu, Xp., Wang, F. et al. Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation, Migration, Invasion and Angiogenesis of Adipose Derived Stem Cells. CURR MED SCI 38, 124–130 (2018). https://doi.org/10.1007/s11596-018-1855-8

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-018-1855-8

Key words

Search

Navigation