Abstract
To obtain water-insoluble silk fibroin (SF) materials, polyethylene glycol diglycidyl ether (PEG-DE) was selected as a crosslinking agent to prepare SF films (blends). The reaction conditions were optimized for the crosslinking of the SF molecules. The hot water stability of the blends was measured using BCA protein assay and gravimetric analysis. The molecular conformation and crystalline structure of the blends were analyzed by FTIR and XRD, respectively. When the mass ratio of SF:PEG-DE was 1.0:0.8, the hot water loss rate of the SF blends was minimized. PEG-DE could induce SF molecules to form β-sheets during the gel reaction process, resulting in improved crystallinity and hot water dissolved resistance of the blend films. In order to demonstrate the cytotoxicity of the chemical reagents used to crosslink SF, L929 cells were seeded on the blend film (SF:PEG-DE = 1:1) and cultured for 3 days. Cells of L929 readily adhered and spread in the fusiform on the blend film resulting in high cell viability. The extracted liquid from the SF porous film did not inhibit cell proliferation, as estimated by the MTT assay.
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References
C Vepari, D L Kaplan. Silk as a Biomaterial[J]. Prog. Polym. Sci., 2007, 32: 991–1 007
P W Madden, J N X Lai, K A George, et al. Human Corneal Endothelial Cell Growth on a Silk Fibroin Membrane[J]. Biomaterials, 2011, 32: 4 076–4 084
G H Altman, R L Horan, H H Lu, et al. Silk Matrix for Tissue Engineered Anterior Cruciate Ligaments[J]. Biomaterials, 2002, 23:4 131–4 141
S Sofia, M B McCarthy, G Gronowicz, et al. Functionalized Silk-Based Biomaterials for Bone Formation[J]. J. Biomed. Mater. Res., 2001, 54: 139–148
M Lovett, G Eng, J A Kluge, et al. Tubular Silk Scaffolds for Small Diameter Vascular Grafts[J]. Organogenesis, 2010, 6: 217–224
J R Mauney, T Nguyen, K Gillen, et al. Engineering Adipose-like Tissue in vitro and in vivo Utilizing Human Bone Marrow and Adipose-Derived Mesenchymal Stem Cells with Silk Fibroin 3D Scaffolds[J]. Biomaterials, 2007, 28: 5 280–5 290
Y Yang, X M Chen, F Ding, et al. Biocompatibility Evaluation of Dilk Fibroin with Peripheral Nerve Tissues and Cells in vitro[J]. Biomaterials, 2007, 28 1 643-1 652
G M Nogueira, A C D Rodas, C A P Leite, et al. Preparation and Characterization of Ethanol-Treated Silk Fibroin Dense Membranes for Biomaterials Application using Waste Silk Fibers as Raw Material[J]. Bioresour. Technol., 2010, 101: 8 446–8 451
G Freddi, G Pessina, M Tsukada. Swelling and Dissolution of Silk Fibroin (Bombyx mori) in N-methyl Morpholine N-oxide[J]. Int. J. Biol. Macromol., 1999, 24: 251–263
P Chellan, R H Nagaraj. Protein Crosslinking by the Maillard Reaction: Dicarbonyl-Derived Imidazolium Crosslinks in Aging and Diabetes[J]. Arch. Biochem. Biophys., 1999, 368:98–104
N W Guldner, I Jasmund, H Zimmermann, et al. Detoxification and Endothelialization of Glutaraldehyde-Fixed Bovine Pericardium with Titanium Coating: a New Technology for Cardiovascular Tissue Engineering[J]. Circulation, 2009, 119: 1 653–1 660
S S Kim, S H Lim, S W Cho, et al. Tissue Engineering of Heart Valves by Recellularization of Glutaraldehyde-Fixed Porcine Valves using Bone Marrow-Derived Cells[J]. Exp. Mol. Med., 2006, 38: 273–283
B Han, J Jaurequi, B W Tang, et al. Proanthocyanidin: a Natural Crosslinking Reagent for Stabilizing Collagen Matrices[J]. J. Biomed. Mater. Res. A, 2003, 65: 118–124
Q Lu, X Hu, X Q Wang, et al. Water-Insoluble Silk Films with Silk I Structure[J]. Acta Biomater., 2010, 6: 1 380–1 387
S J Ling, W Zhou, Z Z Shao, et al. Conformation Transition Kinetics and Spinnability of Regenerated Silk Fibroin with Glycol, Glycerol and Polyethylene Glycol[J]. Mater. Lett., 2012, 81: 13–15
P Slusarewicz, K Zhu, T Hedman. Kinetic Characterization and Comparison of Various Protein Crosslinking Reagents for Matrix Modification[J]. J. Mater. Sci. Mater. Med., 2010, 21: 1 175–1 181
O Bayraktar, Ö Malay, Y Özgarip, et al. Silk Fibroin as a Novel Coating Material for Controlled Release of Theophylline[J]. Biotechnol. Bioeng., 2005, 60: 373–381
L Meinel, S Hofmann, V Karageorgiou, et al. Engineering Cartilagelike Tissue Using Human Mesenchymal Stem Cells and Silk Protein Scaffolds[J]. Biotechnol. Bioeng., 2004, 88: 379–391
J S Chen, G H Altman, V Karageorgiou, et al. Human Bone Marrow Stromal Cell and Ligament Fibroblast Responses on RGD-Modified Silk Fibers[J]. J. Biomed. Mater.Res. A, 2003, 67: 559–570
S Q Yan, Q Zhang, J N Wang, et al. Characterization of Silk Fibroin/Hyaluronic Acid Blend Films Cross-Linked with EDC[J]. J. Fiber Bioeng. Inform., 2010, 3: 62–67
X X Yun, F Liu, Y T Xu, et al. In vitro Study in the Endothelial Cell Compatibility and Endothelialization of Genipin-Crosslinked Biological Tissues for Tissue-Engineered Vascular Scaffolds[J]. J. Mater. Sci. Mater. Med., 2010, 21: 777–785
S S Silva, A Motta, M T Rodrigues, et al. Novel Genipin-Cross-Linked Chitosan/Silk Fibroin Sponges for Cartilage Engineering Strategies[J]. Biomacromolecules, 2008, 9: 2 764–2 774
S S Silva, D Maniglio, A Motta, et al. Genipin Modified Silk-Fibroin Nanometric Nets[J]. Macromol. Biosci., 2008, 8: 766–774
A Motta, B Barbato, P Torricelli, et al. Stabilization of Bombyx mori Silk Fibroin/Sericin Films by Crosslinking with PEG-DE 600 and Genipin[J]. J. Bioactive and Compatible Pol., 2011, 26: 130–143
L Zhou, X Chen, W L Dai, et al. X-ray Photoelectron Spectroscopic and Raman Analysis of Silk Fibroin-Cu (II) Films[J]. Biopolymers, 2006, 82: 144–151
M Z Li, T Wei, S Z Lu, et al. Compliant Film of Regenerated Antheraea pernyi Silk Fibroin by Chemical Crosslinking[J]. Int. J. Biological. Macromol., 2003, 32: 159–163
J N Wang, H G Yi, Y Q Wei. Preliminary Biocompatibility Evaluation of Regenerated Antheraea yamamai Silk Fibroin in vitro[J]. J. Wuhan Univ. Technol., 2011, 26: 1 044–1 048
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Funded by National Natural Science Foundation of China (Nos.51173125 and 51473108), Natural Science Foundation of Jiangsu Province of China (Nos.BK2012633 and BK2041210), College Natural Science Research Project of Jiangsu Province of China (No.12KJA43004), the Science and Technology Development Foundation of Suzhou of China (Nos.SYG201001 and SS201341) and Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD]
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Wei, Y., Sun, D., Yi, H. et al. Preparation and characterization of PEGDE crosslinked silk fibroin film. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 29, 1083–1089 (2014). https://doi.org/10.1007/s11595-014-1047-8
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DOI: https://doi.org/10.1007/s11595-014-1047-8