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The Biopotential of a Wood-decaying Strain of Trichoderma harzianum for Protecting and Promoting the Sensitive Early Roots of Micropropagated Potato Plantlets and Reducing Transplantation Stress in an Aeroponic System

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Abstract

Minituber production in aeroponics is a growing trend in the potato seed industry for ensuring high-quality disease-free seed, as it requires vigorous, healthy plantlets to survive under stressful conditions. Fungi can assist plantlets endure stress, and there is a pressing need to explore various native fungi from an environmentally beneficial standpoint. In this study, three strains of Trichoderma harzianum were tested for their ability to protect the early roots of tissue culture–raised potato plantlets during hardening and to reduce transplantation stress in an aeroponic system. In the bioactivity results, strain TI21 isolated from decayed wood significantly improved root length by 42.7%, shoot length by 25.9% and biomass weight by 29.9% of tomato seedlings compared to control and demonstrated strong biocontrol activity (percentage) against four phytopathogens, including Sclerotium delphinii SCR5 (75.97 ± 8.06), Fusarium equiseti PTR3 (67.27 ± 5.11), Curvularia spicifera BLR4 (61.66 ± 2.98) and Alternaria alternata ALT10 (61.90 ± 4.58). Strains TI26 and TI27, isolated from rice rhizospheric soil, yielded no significant results. In potato-plantlet-hardening experiments, TI21 increased plantlet survival by 22.46%, 25.75% and 24.12%, compared to TI26, TI27, and control, respectively, by reducing root-rot caused by Sclerotium sp. and Fusarium sp. After 7 days of transplantation in aeroponic, TI21-treated plantlets had a significantly higher survival (90.5%) than control (77.6%), but there were no significant differences after 12 days, revealing that plantlets were much more susceptible to transplantation stress within the first 7 days of transplantation, which was overcome by TI21-treated plantlets. According to the findings, strain TI21 can be employed in micropropagated plantlets’ hardening to improve survival rates in aeroponic systems.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by Apex Biofertilizers and Biopesticides Limited, Go-bindaganj-5740, Gaibandha, Bangladesh and Apex Biotechnology laboratory, Apex Holdings Ltd., East Chandora, Shafipur, Kaliakoir, Gazipur 1751, Bangladesh.

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  1. Abuhena, Golam Kabir and Faisal Azim contributed equally to this work.

Authors and Affiliations

  1. Department of Research & Development, Apex Biofertilizers & Biopesticides Limited, Gobindaganj-5740, Gaibandha, Bangladesh

    Md. Abuhena, Md. Golam Kabir, Md. Faisal Azim, Jubair Al-Rashid & Noorain Munim Rasul

  2. Apex Biotechnology Laboratory, Apex Holdings Ltd, East Chandora, Shafipur, Kaliakoir, Gazipur, 1751, Bangladesh

    Md. Golam Kabir, Mohammad Sharif Sarker, Jubair Al-Rashid, Md. Niuz Morshed Khan, Nirmal Chandra Barman & Noorain Munim Rasul

  3. Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, 461-701, Republic of Korea

    Shahina Akter

  4. Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong, Gyeonggi-do, 17546, Republic of Korea

    Md. Amdadul Huq

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  1. Md. Abuhena
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Contributions

M.A., M.G.K. and M.F.A: conceptualization of the research work, data collection, performer of the experimental research work, statistical analysis, manuscript writing, manuscript revision and editing. M.S.S J.A.-R. M.N.M.K. and N.C.B.: performer of the experimental research work, reviewing, editing. N.M.R. and S.A.: manuscript writing, reviewing, editing and, approval of the protocol. M.A.H.: manuscript writing, reviewing, editing and submission of the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Md. Golam Kabir, Shahina Akter or Md. Amdadul Huq.

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Abuhena, M., Kabir, M.G., Azim, M.F. et al. The Biopotential of a Wood-decaying Strain of Trichoderma harzianum for Protecting and Promoting the Sensitive Early Roots of Micropropagated Potato Plantlets and Reducing Transplantation Stress in an Aeroponic System. Potato Res. 66, 663–681 (2023). https://doi.org/10.1007/s11540-022-09594-5

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