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COP9 signalosome subunit PfCsnE regulates secondary metabolism and conidial formation in Pestalotiopsis fici

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Abstract

The COP9 signalosome (CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism (SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type (WT). Transcriptome analysis of WT and ΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters (BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.

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Acknowledgements

Wenbing Yin is a scholar of “the 100 Talents Project” of Chinese Academy of Sciences. This work was supported by the National Key Research and Development Program (2016YFD0400105), National Natural Science Foundation of China (31670402 and 31400334), and Sate Key Laboratory of Mycology Open Project (SKLMKF 2015-1).

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Authors and Affiliations

  1. Zhejiang Provincial (Wenzhou) Key Lab for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China

    Yanjing Zheng, Xiufeng Yan & Huixi Zou

  2. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Yanjing Zheng, Xiaoling Zhang, Wei Li, Gang Liu & Wen-Bing Yin

  3. School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Xiuna Wang & Shihua Wang

  4. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

    Wen-Bing Yin

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  1. Yanjing Zheng
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  2. Xiuna Wang
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  4. Wei Li
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Correspondence to Huixi Zou or Wen-Bing Yin.

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Zheng, Y., Wang, X., Zhang, X. et al. COP9 signalosome subunit PfCsnE regulates secondary metabolism and conidial formation in Pestalotiopsis fici . Sci. China Life Sci. 60, 656–664 (2017). https://doi.org/10.1007/s11427-017-9068-4

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  • DOI: https://doi.org/10.1007/s11427-017-9068-4

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