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Synthesis of aptamer-functionalized Ag nanoclusters for MCF-7 breast cancer cells imaging

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Abstract

Aptamer-functionalized silver nanoclusters (Ag NCs) have been attracting a lot of interest as label-free probes which have been successfully applied to both cell imaging and molecular detection. MUC1 aptamer is an ssDNA aptamer that specifically binds to MUC1 mucin which is a large transmembrane glycoprotein whose expression level increases at least 10-fold in primary and metastatic breast cancers. Using C4A4C3-linker-MUC1 as template, the Ag NCs were synthesized through one-pot process. The fluorescence intensity of Ag NCs was found to be closely related to the length and type (poly adenine or thymine) of the linker, the optimum linker being–AAAAA–. Using the C4A4C3-A5-MUC1 as the scaffold, the synthesized Ag NCs emitted fluorescence with high quantum yield (QY) of 66.5%. Based on the specific interaction between the MUC1 aptamer and MUC1 mucin, the C4A4C3-A5-MUC1-stabilized Ag NCs could recognize and differentiate the MCF-7 breast cancer cells from MDA-MB-231 breast cancer and A549 human lung cancer cells.

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Acknowledgments

This work was supported by the National Basic Research Program of China (2014CB744501), the National Natural Science Foundation of China (61271056, 61527806), the Natural Science Foundation of Jiangsu Province (BK20141332), the National Youth Science Foundation of China (61301043), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China

    Taotao Li, Zeeshan Ali, Zunliang Wang, Xianbo Mou & Nongyue He

  2. School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210096, China

    Jingjing Yang & Zhifei Wang

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  1. Taotao Li
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  2. Jingjing Yang
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  3. Zeeshan Ali
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  4. Zunliang Wang
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  5. Xianbo Mou
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  6. Nongyue He
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  7. Zhifei Wang
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Corresponding authors

Correspondence to Nongyue He or Zhifei Wang.

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Li, T., Yang, J., Ali, Z. et al. Synthesis of aptamer-functionalized Ag nanoclusters for MCF-7 breast cancer cells imaging. Sci. China Chem. 60, 370–376 (2017). https://doi.org/10.1007/s11426-016-0159-2

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  • DOI: https://doi.org/10.1007/s11426-016-0159-2

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