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Study on morphological and genetic diversity of Rehmannia glutinosa cultivated in Japan

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Abstract

It is said that Rehmannia glutinosa is grouped into two types, Akaya and Kaikei, in Japan. However, previous reports of genetic analysis of R. glutinosa in commercial products suggest the existence of varieties other than these two, and therefore, it is inappropriate to simply classify them into these two varieties. In this study, we clarified the diversity of R. glutinosa cultivated in Japan on the basis of morphological observation and genetic analysis. We conducted principal component analysis (PCA) of R. glutinosa morphology, including leaf surface color, leaf undersurface anthocyanin coloration, root shape, and the ratio of string root. We also performed (1) sequence-related amplified polymorphism (SRAP) analysis and (2) polymorphism analysis of the TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) gene region. We were able to separate Akaya type from Kaikei type, and to divide Kaikei type into three small groups. These two gene analysis methods were also useful in estimating the patrilineal and matrilineal strains of a hybrid origin. Our findings revealed that Akaya type and Kaikei type can be distinguished on the basis of morphological and genetic analyses, and that Kaikei type cultivated in Japan exhibited morphological and genetic diversity.

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Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research (C), No. 18K06730, for 2018-2020 from the Japan Society for the Promotion of Science. We thank Dr. Eiichi Kodaira, Medicinal Plant Garden, School of Pharmacy, Kitasato University; University of Toyama; Iwakuni Hongo Laboratory of Shinnihoniyaku Co., Ltd.; Takeda Garden for Medicinal Plant Conservation, Kyoto; and Tokyo Metropolitan Medicinal Plants Garden for kind help in sample collection.

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Authors and Affiliations

  1. Laboratory of Molecular Pharmacognosy, Division of Pharmaceutical Sciences, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan

    Yuina Yoshie, Hirokazu Ando, Kazuki Yoshihara & Yohei Sasaki

  2. Fukuda Shoten, 477 Abe, Sakurai, Nara, 633-0054, Japan

    Kozo Fukuda

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  1. Yuina Yoshie
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  2. Hirokazu Ando
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  3. Kazuki Yoshihara
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  4. Kozo Fukuda
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  5. Yohei Sasaki
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Correspondence to Yohei Sasaki.

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Yoshie, Y., Ando, H., Yoshihara, K. et al. Study on morphological and genetic diversity of Rehmannia glutinosa cultivated in Japan. J Nat Med 76, 352–366 (2022). https://doi.org/10.1007/s11418-021-01587-x

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