Abstract
We attempted to conduct an intraspecific analysis of 30 peony cultivars in Japan and to authenticate five herbal medicine samples derived from Paeoniae Radix by polymorphism analysis of the TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) gene region. We focused on cultivar-dependent differences in leaf margin undulation and analyzed the sequence of the related TCP gene region. As a result, we found that the nucleotide sequences of 29 cultivars of Paeonia lactiflora except ‘America’ exhibit interspecific variations compared with the nucleotide sequences of Paeonia suffruticosa and Paeonia tenuifolia. Therefore, in the dendrogram constructed on the basis of the sequence similarity in the TCP gene region, the 29 cultivars of P. lactiflora were separated from P. suffruticosa, P. tenuifolia, and ‘America’, and clustered into three subgroups. There were 16 variations containing heterogenous DNA sequences within P. lactiflora species, and two characteristic variations in subgroup I. Some P. lactiflora cultivars showed the same nucleotide sequence in the TCP gene region, whereas the five herbal medicine samples showed different sequences, although all of them could be authenticated. The results suggest that Paeoniae Radix in the Japanese crude drug market can be authenticated by analysis of the TCP gene region.
Graphical abstract
References
Fukuda S (1991) Production and resources of Paeonia. J Tradit Sino-Jpn Med 12:77–85
Ministry of Health, Labour and Welfare (2016) The Japanese pharmacopoeia seventeenth edition (Ministry Notification No. 64 of Mar. 7, 2016). The Ministry of Health, Labour and Welfare, Japan
Yamamoto Y, Ko H, Sasaki H, Takeda O, Higuchi Y, Mukaida Y, Mori Y, Yamaguchi Y, Shiratori M (2019) Survey on crude drug usage in Japan. Jpn J Pharmacol 73:16–35
Ministry of Health and Welfare (1994) Yakuyoushokubutsu saibai to hinshitsuhyoka Part 3. Yakuji Nippo Limited, Tokyo, pp 45–56
Shimada-Takaura K, Nakamura Y, Kawase M, Komatsu K, Takahashi K (2018) Quality characterization of Japanese medicinal Paeoniae Radix by metallomic analysis. Chem Pharm Bull 66:353–357
Tarachiwin L, Katoh A, Ute K, Fukusaki E (2008) Pyrolysis GC-MS-based metabolite fingerprinting for quality evaluation of commercial Angelica acutiloba roots. J Pharm Biomed Anal 48:42–48
Sun YL, Hong SK (2011) Genetic diversity and phylogenetic analysis of genus Paeonia based on nuclear ribosomal DNA ITS sequence. J Plant Biotechnol 38:234–240
Li Q, Xu JU, Han L, Gao CR, Lu J, Du GC, Sun X (2017) Evaluation of ITS2 for intraspecific identification of Paeonia lactiflora cultivars. Biotechnol Rep 15:101–106
Sun YL, Hong SK (2012) Phylogenetic relationship and evolution analysis of the peony Paeonia species using multi-locus deoxyribonucleic acid (DNA) barcodes. J Med Plants Res 6:5048–5058
Kim WJ, Ji Y, Choi G, Kang YM, Yang S, Moon BC (2016) Molecular identification and phylogenetic analysis of important medicinal plant species in genus Paeonia based on rDNA-ITS, matK, and rbcL DNA barcode sequences. Genet Mol Res 15(10):4238
Sang T, Daniel JC, Tod FS (1997) Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Am J Bot 84:1120–1136
Zhu S, Yu XL, Wu YQ, Shiraishi F, Kawahara N, Komatsu K (2015) Genetic and chemical characterization of white and red peony root derived from Paeonia lactiflora. J Nat Med 69:35–45
Hosoki T, Nagasato T, Kimura D, Nishimoto K, Hasegawa R, Ohta K, Sugiyama M, Haruki K (1997) Classification of herbaceous peony cultivars by random amplified polymorphic DNA (RAPD) analysis. J Japan Soc Hortic Sci 65:843–849
Varkulevičienė J, Žukauskienė J (2014) Analysis of peonies cultivars and their parental forms using RAPD primers. Biologija 60:188–195
Xu JU, Dou X, Gao CR, Lu J, Yan LP (2018) Genetic diversity of 20 cut flower cultivars of Paeonia lactiflora with AFLP analysis. Chin Agric Sci Bull 10:65–69
Li L, Cheng FY, Zhang QX (2011) Microsatellite markers for the chinese herbaceous peony Paeonia lactiflora (Paeoniaceae). Am J Bot 98:16–18
Sun J, Yuan JX, Wang BS, Pan J, Zhang DM (2011) Development and characterization of 10 microsatellite loci in Paeonia lactiflora (Paeoniaceae). Am J Bot 98:242–243
Cheng Y, Kim CH, Shin DI, Kim SM, Koo HM, Park YJ (2011) Development of simple sequence repeat (SSR) markers to study diversity in the herbaceous peony (Paeonia lactiflora). J Med Plants Res 5:6744–6751
Hosoki T, Seo M, Hamada M, Itoh K, Inaba K (1991) New classification method of herbaceous peony cultivars based on morphological characters and distribution pattern of flavone/flavonol components in the petals. J Japan Soc Hortic Sci 59:787–793
Koyama T, Mitsuba N, Seki M, Shinozaki K, Ohme-Takagi M (2010) TCP transcription factors regulate the activities of ASYMMETRIC LEAVES1 and miR164, as well as the auxin response, during differentiation of leaves in Arabidopsis. Plant Cell 22:3574–3588
Li Y, Lu JX, Chang YH, Tang WL, Yang QS (2017) Comparative analysis of tree peony petal development by transcriptome sequencing. Acta Physiol Plant 39:216
Tamura T, Tanaka A, Uchi M, Murakami M (2010) Studies on cultivation of peony—qualitative analysis of Paeoniae root cultivated for 8 years—annual report of Toyama prefectural institute for pharmaceutical research 37:57–63
Ichimura K (2013) Kakiryutu saishin no doukou. Bull Natl Inst Flor Sci 13:1–15
Shimada K, Nakamura Y, Kawase M, Komatsu K, Saito T, Takahashi K (2014) Establishment of a methodology for identifying Paeoniae Radix based on metallomics analysis. J Nat Med 68:407–413
CBOL Plant Working Group (2009) A DNA barcode for land plants. Proc Natl Acad Sci 106:12794–12797
Cubas P, Lauter N, Doebley J, Coen E (1999) The TCP domain: a motif found in proteins regulating plant growth and development. Plant J 18:215–222
Acknowledgement
This work was supported by a Grant-in-Aid for Scientific Research (C), No. 18K06730, for 2018–2020 from the Japan Society for the Promotion of Science.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Yoshie, Y., Ando, H., Tamura, T. et al. Polymorphism analysis of TCP gene region to intraspecific analysis of Paeonia lactiflora, and application for authentication of Paeoniae Radix. J Nat Med 75, 985–993 (2021). https://doi.org/10.1007/s11418-021-01521-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11418-021-01521-1