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Ellagitannins from Rosa roxburghii suppress poly(I:C)-induced IL-8 production in human keratinocytes

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Abstract

The anti-inflammatory effects of a 50% aqueous extract of Rosa roxburghii fruit (RRFE) and two ellagitannins (strictinin and casuarictin) isolated from the RRFE were evaluated in a cell model of skin inflammation induced by self-RNA released from epidermal cells damaged by UV ray (UVR) irradiation. The RRFE inhibited interleukin-8 (IL-8) mRNA expression in normal human epidermal keratinocytes (NHEKs) stimulated with polyinosinic:polycytidylic acid (poly(I:C)), a ligand of toll-like receptor-3 (TLR-3). The plant-derived anti-inflammatory agents, dipotassium glycyrrhizinate (GK2) and allantoin, had no influence on the IL-8 expression. The purified compounds, strictinin and casuarictin, inhibited the IL-8 mRNA expression and IL-8 release induced in NHEKs by poly(I:C). These ellagitannins were thus found to be responsible for the biological activity exhibited by the RRFE. This study demonstrates that RRFE and isolated RRFE compounds show promise as ingredients for products formulated to improve skin disorders induced by UVR irradiation.

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Acknowledgements

We are grateful to Prof. Hiroshi Ueda at Gifu University for his critical discussion and advice on the preparation of this manuscript. We would like to thank Mr. Simon Johnson for his skillful assistance in the preparation of this manuscript.

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Authors and Affiliations

  1. Medical Science Division, United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 1-1 Yanagido, Gifu City, Gifu, 501-1194, Japan

    Satoru Takayama & Hiroyuki Tanaka

  2. Research and Development Department, Ichimaru Pharcos Co., Ltd, 318-1 Asagi, Motosu City, Gifu, 501-0475, Japan

    Satoru Takayama, Hiroyuki Kojima, Teruaki Masutani & Arunasiri Iddamalgoda

  3. Department of Applied Life Science, Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu City, Gifu, 501-1194, Japan

    Miho Kawanishi, Kosei Yamauchi & Tohru Mitsunaga

  4. Laboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu City, Gifu, 501-1196, Japan

    Daiki Tokumitsu & Hiroyuki Tanaka

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  1. Satoru Takayama
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Takayama, S., Kawanishi, M., Yamauchi, K. et al. Ellagitannins from Rosa roxburghii suppress poly(I:C)-induced IL-8 production in human keratinocytes. J Nat Med 75, 623–632 (2021). https://doi.org/10.1007/s11418-021-01509-x

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