Abstract
Layered double hydroxide (LDH) nanomaterials are utilized extensively in numerous fields because of their distinctive structural properties. It is critical to understand the environmental behavior and toxicological effects of LDHs to address potential concerns caused by their release into the environment. In this work, the toxicological effects of two typical LDHs (Mg-Al-LDH and Zn-Al-LDH) on freshwater green algae (Scenedesmus obliquus) and the main affecting factors were examined. The Zn-Al-LDH exhibited a stronger growth inhibition toxicity than the Mg-Al-LDH in terms of median effect concentration. This toxicity difference was connected to the stability of particle dispersion in water and the metallic composition of LDHs. The contribution of the dissolved metal ions to the overall toxicity of the LDHs was lower than that of their particulate forms. Moreover, the joint toxic action of different dissolved metal ions in each LDH belonged to additive effects. The Mg-Al-LDH induced a stronger oxidative stress effect in algal cells than the Zn-Al-LDH, and mitochondrion was the main site of LDH-induced production of reactive oxygen species. Scanning electron microscope observation indicated that both LDHs caused severe damage to the algal cell surface. At environmentally relevant concentrations, the LDHs exhibited joint toxic actions with two co-occurring contaminants (oxytetracycline and nano-titanium dioxide) on S. obliquus in an additive manner mainly. These findings emphasize the impacts of the intrinsic nature of LDHs, the aqueous stability of LDHs, and other environmental contaminants on their ecotoxicological effects.
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We thank the anonymous reviewers for helping to improve the manuscript.
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This work was supported by the National Natural Science Foundation of China (Grant number 31971522) and the Qing Lan Project of Jiangsu Province (Grant number R2021Q03).
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LY: investigation, data curation, formal analysis, and writing—original draft preparation. ZW: conceptualization, methodology, resources, investigation, data curation, formal analysis, writing—review and editing, visualization, funding acquisition, and project administration. DW: formal analysis and writing—review and editing. All authors read and approved the final manuscript.
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Yu, L., Wang, Z. & Wang, DG. Factors affecting the toxicity and oxidative stress of layered double hydroxide-based nanomaterials in freshwater algae. Environ Sci Pollut Res 30, 63109–63120 (2023). https://doi.org/10.1007/s11356-023-26522-3
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DOI: https://doi.org/10.1007/s11356-023-26522-3