Abstract
Paraquat (PQ) is an organic compound, which is commonly used as a herbicide in the agriculture sector, and it is also known to stimulate critical damages in the male reproductive system. Gossypetin (GPTN) is one of important members of the flavonoid family, which is an essential compound in flowers and calyx of Hibiscus sabdariffa with potential pharmacological properties. The current investigation was aimed to examine the ameliorative potential of GPTN against PQ-instigated testicular damages. Adult male Sprague–Dawley rats (n = 48) were distributed into four groups: control, PQ (5 mg/kg), PQ + GPTN (5 mg/kg + 30 mg/kg respectively), and GPTN (30 mg/kg). After 56 days of treatment, biochemical, spermatogenic indices, hormonal, steroidogenic, pro-or-anti-apoptotic, and histopathological parameters were estimated. PQ exposure disturbed the biochemical profile by reducing the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GSR), while it increased the concentration of reactive oxygen species (ROS) and malondialdehyde (MDA) level. Furthermore, PQ exposure decreased the sperm motility, viability, number of hypo-osmotic tail swelled spermatozoa, and epididymal sperm count; additionally, it increased sperm morphological (head mid-piece and tail) abnormalities. Moreover, PQ lessened the follicle-stimulating hormone (FSH), luteinizing hormone (LH), and plasma testosterone levels. Besides, PQ-intoxication downregulated the gene expression of steroidogenic enzymes (StAR, 3β-HSD, and 17β-HSD) and anti-apoptotic marker (Bcl-2), whereas upregulated the gene expression of apoptotic markers (Bax and Caspase-3). PQ exposure led to histopathological damages in testicular tissues as well. Nonetheless, GPTN inverted all the illustrated impairments in testes. Taken together, GPTN could potently ameliorate PQ-induced reproductive dysfunctions due to its antioxidant, androgenic, and anti-apoptotic potential.
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Data availability
The datasets used/analyzed in this study are available from the corresponding author on reasonable request.
The study was approved by the Institutional Biosafety/Bioethics Committee (IBC) of the University of Agriculture, Faisalabad, in compliance with this (CEE Council 86/609) protocol.
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SM and MUI conceived the idea and designed the study. MUI, SM, HA1, and SI performed the experiments. HA2 and SI helped in statistical analysis. MUI, SM, and QA wrote the manuscript. All the authors read and approved the final version of manuscript.
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Mustafa, S., Anwar, H., Ain, Q.u. et al. Therapeutic effect of gossypetin against paraquat-induced testicular damage in male rats: a histological and biochemical study. Environ Sci Pollut Res 30, 62237–62248 (2023). https://doi.org/10.1007/s11356-023-26469-5
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DOI: https://doi.org/10.1007/s11356-023-26469-5