Abstract
This study aimed to investigate the impact of ozonation on inactivation of antibiotic-resistant bacteria (ARB) including E. coli, P. aeruginosa, and A. baumannii, as well as on removal of 16S-rRNA gene and their associated antibiotic-resistant genes (ARGs) indigenously present in effluent of municipal wastewater treatment plant. The Chick-Watson model was used to describe bacterial inactivation rates at specific ozone doses. Maximum reduction of total cultivable A. baumannii, E. coli, and P. aeruginosa were found to be 7.6, 7.1, and 4.7 log, respectively, with the highest ozone dose of 0.48 gO3/gCOD at 12 min contact time. According to the study results, complete inactivation of ARB and bacterial regrowth was not observed after 72 h incubation. The culture methods overestimated the performance of disinfection processes and propidium monoazide combined with qPCR, and showed the presence of viable but non-culturable bacteria after ozonation. ARGs were more persistent to ozone than ARB. The results of this study highlighted the significance of specific ozone dose and contact time in ozonation process considering the bacterial species and associated ARGs as well as the wastewater physicochemical characteristics, in order to help diminish the entrance of the biological microcontaminants into the environment.
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The data that support the findings of this study are available on request from the corresponding author.
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Acknowledgements
We would like to thank all staffs from Tabriz municipal WWTP for substantial help during sampling campaigns and helpful advice. We thank Mr. Mohammad Reza Farshchian and Mr. Mohammad Abedpour from Department of Environmental Health Engineering for help with bacterial and chemical analyses.
Funding
This study was supported by the Health and Environment Research Center, Tabriz University of Medical Sciences, Tabriz, Iran (Grant No. 62250).
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AR: methodology, investigation, and writing original draft. RD: conceptualization, methodology, writing/review and editing, project administration, and funding acquisition. DF: methodology, investigation, writing/review, and editing. HA: methodology and investigation. MM: methodology and investigation. SM: statistical analysis of data. DS: methodology and investigation. FB: investigation. All authors read and approved the final manuscript.
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Rajabi, A., Farajzadeh, D., Dehghanzadeh, R. et al. Optimizing ozone dose and contact time for removal of antibiotic-resistant P. aeruginosa, A. baumannii, E. coli, and associated resistant genes in effluent of an activated sludge process in a municipal WWTP. Environ Sci Pollut Res 30, 55569–55581 (2023). https://doi.org/10.1007/s11356-023-26270-4
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DOI: https://doi.org/10.1007/s11356-023-26270-4