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Iron ore or manganese ore filled constructed wetlands enhanced removal performance and changed removal process of nitrogen under sulfamethoxazole and trimethoprim stress

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Abstract

Iron ore and manganese ore were used as substrate of constructed wetlands (CWs) to enhance nitrogen (N) removal. However, the N purification performance in CWs filled with iron or manganese ore under antibiotics stress needs further study. In this study, three groups of CWs filled with river sand, limonite (a kind of iron ore), and manganese ore sand were constructed, which were named as C-CWs, Fe-CWs, and Mn-CWs, respectively. The effect and mechanism of the composite antibiotics sulfamethoxazole (SMX) and trimethoprim (TMP) on N removal in CWs were investigated. While the addition of SMX and TMP inhibited about 40% nitrification and promoted about 25% denitrification in all CWs, Fe-CWs and Mn-CWs always had better N removal performance than C-CWs. Changes in microbial community structure in CWs indicated that the better N removal performance in Fe-CWs and Mn-CWs was attributed to the presence of more abundant and diverse N-associated bacteria, especially Fe- and Mn-driven autotrophic denitrifying bacteria. What’s more, the addition of iron ore or manganese ore contributed to the better N removal performance with highest relative abundance of N-transferring bacteria under antibiotics stress.

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Funding

This study was supported by the National Natural Science Foundation of China (No.51978385, 51720105013), Shandong Provincial Key Research and Development Program (Major Scientific and Technological Innovation Project) (NO. 2020CXGC011406, 2019JZZY010411), and Shandong University Interdisciplinary Research and Innovation Team of Young Scholars (2020QNQT20).

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Authors and Affiliations

  1. Environment Research Institute, Shandong University, Qingdao, 266237, China

    Junyu Huo, Xiaojin Hu & Huijun Xie

  2. Jiangsu Surveying and Design Institute of Water Resource Co.,Ltd, Yangzhou, 225127, China

    Chaoyu Li

  3. Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & Engineering, Shandong University, Qingdao, 266237, China

    Zhen Hu, Haiming Wu, Shuang Liang & Jian Zhang

  4. College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, China

    Jian Zhang

Authors
  1. Junyu Huo
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  2. Chaoyu Li
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  3. Xiaojin Hu
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  4. Huijun Xie
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  5. Zhen Hu
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  6. Haiming Wu
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  7. Shuang Liang
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  8. Jian Zhang
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Contributions

JH: conceptualization, investigation, data curation, formal analysis, methodology, writing—original draft. CL: data curation, formal analysis, methodology. XH: formal analysis, methodology, writing—review and Editing. HX: conceptualization, methodology, supervision, funding acquisition, writing—review and editing. ZH: methodology, writing—review and editing. HW: methodology, writing—review and editing. SL: methodology, writing—review and editing. JZ: methodology, writing—review and editing.

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Correspondence to Huijun Xie.

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Huo, J., Li, C., Hu, X. et al. Iron ore or manganese ore filled constructed wetlands enhanced removal performance and changed removal process of nitrogen under sulfamethoxazole and trimethoprim stress. Environ Sci Pollut Res 29, 71766–71773 (2022). https://doi.org/10.1007/s11356-022-20103-6

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  • DOI: https://doi.org/10.1007/s11356-022-20103-6

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