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Effects of Herbaspirillum sp. p5-19 assisted with alien soil improvement on the phytoremediation of copper tailings by Vetiveria zizanioides L.

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Abstract

Microbial assisted phytoremediation and reclamation are both potential contaminated soil remediation technologies, but little is known about the combined application of the two technologies on real contaminated soils. This study investigated the potential of Herbaspirillum sp. p5-19 (p5-19) assisted with alien soil improvement on improving stress tolerance and enhancing the accumulation of Mn, Cu, Zn, and Cd by Vetiveria zizanioides L. in copper tailings. Phytoremediation potential was evaluated by plant biomass and the ability of plants to absorb and transfer heavy metals. Results showed that the biomass was increased by 19.64–173.81% in p5-19 inoculation treatments with and without alien soil improvement compared with control. Meanwhile, photosynthetic pigment contents were enhanced in co-inoculation treatment (p5-19 with alien soil improvement). In addition, the malondialdehyde (MDA) content was decreased, and the activities of antioxidant enzymes such as ascorbate peroxidase (APX), superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were increased in p5-19 treatment, thereby alleviating the oxidative stress. Moreover, co-inoculation significantly (p < 0.05) increased the concentrations of Mn, Cu, Zn, and Cd in the roots and shoots of V. zizanioides. In particular, the highest concentrations of Mn, Zn, and Cd in the shoots (roots) were obtained in covering 10 cm combined with p5-19 inoculation treatment, which were 4.44- (2.71-), 4.73- (3.87-), and 5.93- (4.35-) fold as that of the controls, respectively. These results provided basis for the change of phytoremediation ability of V. zizanioides after inoculation. We concluded that p5-19 assisted with alien soil improvement was a potential strategy for enhancing phytoremediation ability in tailings.

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Acknowledgements

This work was financially supported by Key Research and Development Projects in Anhui Province (201904a020027).

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Authors and Affiliations

  1. College of Resources and Environmental Engineering, Anhui University, Hefei, 230601, China

    Qing Fang, Tao Huang, Ning Wang, Ziwei Ding & Qingye Sun

  2. Key Laboratory of Wetland Ecological Protection and Restoration, Hefei, China

    Qing Fang, Tao Huang, Ning Wang, Ziwei Ding & Qingye Sun

  3. Mining Environmental Restoration and Wetland Ecological Security Collaborative Innovation Center, Hefei, China

    Qing Fang, Tao Huang, Ning Wang, Ziwei Ding & Qingye Sun

  4. Academy of Resources and Environmental Engineering, Anhui University, 111 JiuLong Road, 523, Hefei, 230601, Anhui, People’s Republic of China

    Qingye Sun

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  1. Qing Fang
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Contributions

Qing Fang: conceptualization, investigation, software, formal analysis, writing—original draft

Tao Huang: software, investigation, methodology

Ning Wang: investigation, formal analysis

Ziwei Ding: investigation

Qingye Sun: methodology, funding acquisition, project administration

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Correspondence to Qingye Sun.

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Responsible Editor: Elena Maestri

Highlights

● Herbaspirillum sp. p5-19 aleviates metal toxicity to vetiver grass.

● A combination of p5-19 and alien soil improvement enhanced vetiver grass tolerance.

● Herbaspirillum sp. p5-19 inoculation improved the phytoremediation of tailings.

● C10B remarkably enhanced plant biomass and Mn, Zn, and Cd accumulation.

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Fang, Q., Huang, T., Wang, N. et al. Effects of Herbaspirillum sp. p5-19 assisted with alien soil improvement on the phytoremediation of copper tailings by Vetiveria zizanioides L.. Environ Sci Pollut Res 28, 64757–64768 (2021). https://doi.org/10.1007/s11356-021-15091-y

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