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Effects of nickel oxide nanoparticles on survival, reproduction, and oxidative stress biomarkers in the marine calanoid copepod Centropages ponticus under short-term exposure

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Abstract

Excessive use of nickel oxide nanoparticles (NiO NPs) in various industrial and commercial products can lead to various negative effects in human and environmental health due to their possible discharge into the environment. Nerveless, information about their ecotoxicological effects on marine organisms are lacking. Copepods are good ecotoxicological models because of their high sensitivity to environmental stress and their key role in the marine food webs. In this study, 48 h acute tests were conducted on the marine planktonic copepod Centropages ponticus to assess lethal and sublethal toxicities of NiO NPs. The results revealed LC50 (48 h) of 4 mg/L for adult females. Aggregation and settling of NiO NPs were observed at concentrations ≥ 2 mg/L. Exposure to sublethal concentrations (≥ 0.02 mg/L for 48 h) had significant negative effects on reproductive success in C. ponticus. Egg production after 24 h and 48 h decreased by 32% and 46%, respectively at 0.02 mg/L and 70% and 82%, respectively, at 2 mg/L. Hatching success was reduced by 70% and 79% at 2 mg/L for eggs produced after 24 h and 48 h respectively. Antioxidant enzymatic activity increased significantly with NiO NP concentration and time, indicating that NiO NPs can cause oxidative stress in C. ponticus even under short-term exposure, while significant inhibition of acetylcholinesterase activity at 2 mg/L after 48 h suggests neurotoxic effects of NiO NPs.

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Acknowledgments

The authors would like to thank the members of the Laboratory for Environmental Biomonitoring (Faculty of Sciences of Bizerte; Tunisia), especially Professor Mohamed Dalleli and Ms. Wiem Saidani, for their help in biomarker analysis. We are also grateful to the Institute of Fishing and Aquaculture (ISPA) (Bizerte, Tunisia), especially Professor Mohammed Chalghaf, for algae supply. Special thanks to Professor Abdelhak Othmani for providing nanoparticles. We would like to thank Mary McAfee for the English correction and improvement.

Funding

This research is part of the PhD work of Ms. Emna Djebbi, which is co-funded by the University of Carthage (Bourse d’Alternance) and IRD-Tunisia (Institut de Recherche pour le Développement). The funders had no role in the study design, analysis or interpretation of the data, writing the manuscript, or the decision to submit the paper for publication.

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Authors and Affiliations

  1. Faculty of Sciences of Bizerte, Carthage University, 7021, Zarzouna, LR18ES41 (Tunis El Manar University), 1082, Tunis, Tunisia

    Emna Djebbi

  2. Univ. Montpellier, CNRS, Ifremer, IRD, MARBEC, Montpellier, France

    Delphine Bonnet

  3. Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO UM 110, 13288, Marseille, France

    Olivier Pringault

  4. LEBPAO, Faculty of Sciences of Tunis, University of Tunis, El Manar, FSB, Zarzouna, 7021, Bizerte, Tunisia

    Khawla Tlili

  5. Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, PO Box 2713, Doha, Qatar

    Mohamed Néjib Daly Yahia

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  1. Emna Djebbi
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  2. Delphine Bonnet
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  3. Olivier Pringault
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  4. Khawla Tlili
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Contributions

Emna Djebbi and Mohamed Néjib Daly Yahia contributed to the conception and design of work. Emna Djebbi and Delphine Bonnet contributed to data acquisition and analysis. The first draft of the manuscript was written by Emna Djebbi (PhD student). Delphine Bonnet commented and revised previous versions of the manuscript. Delphine Bonnet, Olivier Pringault, Khaoula Tlili, and Mohamed Néjib Daly Yahia critically revised and approved the manuscript before submission.

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Correspondence to Emna Djebbi.

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Djebbi, E., Bonnet, D., Pringault, O. et al. Effects of nickel oxide nanoparticles on survival, reproduction, and oxidative stress biomarkers in the marine calanoid copepod Centropages ponticus under short-term exposure. Environ Sci Pollut Res 28, 21978–21990 (2021). https://doi.org/10.1007/s11356-020-11781-1

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