Abstract
Nitric oxide (NO) and nitrous oxide (N2O) production in biological nutrient removal has been studied widely due to the strong negative effects on the environment. Nitrite-denitrifying phosphorus removal (N-DPR), as a significant source of NO and N2O production, has received great attention. However, the mechanism of NO and N2O production at different phosphorus concentrations is not well understood. Therefore, this study was conducted to investigate the effect of phosphorus concentration on pollutant removal, as well as NO and N2O production during the N-DPR process. The results showed that the phosphorus removal efficiency was improved with the increase of phosphorus concentration, which is caused by the enrichment of denitrifying phosphorus accumulating organisms (DPAOs) at high phosphorus concentration. High NO production was observed at phosphorus concentration of 0.5 mg L−1, which is mainly attributed to the slow recovery of reductase activity and low abundance of DPAOs. The maximal N2O accumulation of 31.45 mg L−1 was also achieved at phosphorus concentration of 0.5 mg L−1. The possible reason is that fewer poly-β-hydroxyalkanoates (PHAs) were synthesized by glycogen accumulating organisms (GAOs) at low phosphorus concentration, which could intensify the electron competition among different reductases. In addition, free nitrous acid (FNA) inhibition was another significant reason for high N2O production.
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References
APHA (2005) Standard methods for the examination of water and wastewater American Public Health Association (APHA). USA, Washington DC
Bao Z, Ribera-Guardia A, Spinelli M, Sun D, Pijuan M (2018) The effect of temperature shifts on N2O and NO emissions from a partial nitritation reactor treating reject wastewater. Chemosphere 212:162–169. https://doi.org/10.1016/j.chemosphere.2018.08.090
Bergaust L, Mao Y, Bakken LR, Frostegard A (2010) Denitrification response patterns during the transition to anoxic respiration and posttranscriptional effects of suboptimal pH on nitrous oxide reductase in Paracoccus denitrificans. Appl Environ Microbiol 76:6387–6396. https://doi.org/10.1128/AEM.00608-10
Chen Z, Lei X, Lai Q, Li Y, Zhang B, Zhang J, Zhang H, Yang L, Zheng W, Tian Y, Yu Z, Xu H, Zheng T (2014) Phaeodactylibacter xiamenensis gen. nov., sp. nov., a member of the family Saprospiraceae isolated from the marine alga Phaeodactylum tricornutum. Int J Syst Evol Microbiol 64:3496–3502. https://doi.org/10.1099/ijs.0.063909-0
Hirayama H, Takai K, Inagaki F, Nealson KH, Horikoshi K (2005) Thiobacter subterraneus gen. nov., sp. nov., an obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium from a subsurface hot aquifer. Int J Syst Evol Microbiol 55:467–472. https://doi.org/10.1099/ijs.0.63389-0
Huang J, Zhou Z, Zheng Y, Sun X, Yu S, Zhao X, Yang A, Wu C, Wang Z (2019) Biological nutrient removal in the anaerobic side-stream reactor coupled membrane bioreactors for sludge reduction. Bioresour Technol 295:122241. https://doi.org/10.1016/j.biortech.2019.122241
Jia W, Liang S, Ngo HH, Guo W, Zhang J, Wang R, Zou Y (2013) Effect of phosphorus load on nutrients removal and N2O emission during low-oxygen simultaneous nitrification and denitrification process. Bioresour Technol 141:123–130. https://doi.org/10.1016/j.biortech.2013.02.095
Ki BM, Huh IA, Choi JH, Cho KS (2018) Relationship of nutrient dynamics and bacterial community structure at the water-sediment interface using a benthic chamber experiment. J Environ Sci Health 53:482–491. https://doi.org/10.1080/10934529.2017.1412191
Kuba T, Loosderecht MCMV, Heijnen JJ (1996) Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system. Water Res 30:1702–1710. https://doi.org/10.1016/0043-1354(96)00050-4
Li C, Liu S, Ma T, Zheng M, Ni J (2019) Simultaneous nitrification, denitrification and phosphorus removal in a sequencing batch reactor (SBR) under low temperature. Chemosphere 229:132–141. https://doi.org/10.1016/j.chemosphere.2019.04.185
Li C, Wang T, Zheng N, Zhang J, Ngo HH, Guo W, Liang S (2013) Influence of organic shock loads on the production of N2O in denitrifying phosphorus removal process. Bioresour Technol 141:160–166. https://doi.org/10.1016/j.biortech.2013.03.117
Li S, Fei X, Chi Y, Jiao X, Wang L (2018) Integrated temperature and DO effect on the lab scale A2O process: performance, kinetics and microbial community. Int Biodeterior Biodegr 133:170–179. https://doi.org/10.1016/j.ibiod.2018.07.006
Li X, Li Y, Lv D, Li Y, Wu J (2020) Nitrogen and phosphorus removal performance and bacterial communities in a multi-stage surface flow constructed wetland treating rural domestic sewage. Sci Total Environ 709:136235. https://doi.org/10.1016/j.scitotenv.2019.136235
Liu J, Lu Z, Zhang J, Xing M, Yang J (2013) Phylogenetic characterization of microbial communities in a full-scale vermifilter treating rural domestic sewage. Ecol Eng 61:100–109. https://doi.org/10.1016/j.ecoleng.2013.09.015
Lyu W, Huang L, Xiao G, Chen Y (2017) Effects of carbon sources and COD/N ratio on N2O emissions in subsurface flow constructed wetlands. Bioresour Technol 245:171–181. https://doi.org/10.1016/j.biortech.2017.08.056
Miao Z, Li D, Guo S, Zhao Z, Fang X, Wen X, Wan J, Li A (2018) Effect of free nitrous acid on nitrous oxide production and denitrifying phosphorus removal by polyphosphorus-accumulating organisms in wastewater treatment. Biomed Res Int 2018:9192607–9192610. https://doi.org/10.1155/2018/9192607
Miao Z, Zeng W, Wang S, Peng Y, Cao G, Weng D, Xue G, Yang Q (2014) Effect of temperature on anoxic metabolism of nitrites to nitrous oxide by polyphosphate accumulating organisms. J Environ Sci 26:264–273. https://doi.org/10.1016/s1001-0742(13)60406-4
Mino T, Loosdrecht MCMV, Heijnen JJ (1998) Microbiology and biochemistry of the enhanced biological phosphate removal process. Water Res 32:3193–3207. https://doi.org/10.1016/S0043-1354(98)00129-8
Ni BJ, Yuan Z (2013) A model-based assessment of nitric oxide and nitrous oxide production in membrane-aerated autotrophic nitrogen removal biofilm systems. J Membrane Sci 428:163–171. https://doi.org/10.1016/j.memsci.2012.10.049
Pan Y, Ye L, Ni BJ, Yuan Z (2012) Effect of pH on N2O reduction and accumulation during denitrification by methanol utilizing denitrifiers. Water Res 46:4832–4840. https://doi.org/10.1016/j.watres.2012.06.003
Ribera-Guardia A, Marques R, Arangio C, Carvalheira M, Oehmen A, Pijuan M (2016) Distinctive denitrifying capabilities lead to differences in N2O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms. Bioresour Technol 219:106–113. https://doi.org/10.1016/j.biortech.2016.07.092
Seviour RJ, Mino T, Onuki M (2003) The microbiology of biological phosphorus removal in activated sludge systems. FEMS Microbiol Rev 27:99–127. https://doi.org/10.1007/s12275-008-0051-0
Thiel J, Spring S, Tindall BJ, Spröer C, Bunk B, Koeksoy E, Ngugi DK, Schink B, Pester M (2020) Desulfolutivibrio sulfoxidireducens gen. nov., sp. nov., isolated from a pyrite-forming enrichment culture and reclassification of Desulfovibrio sulfodismutans as Desulfolutivibrio sulfodismutans comb. nov. Syst and Appl Microbiol 43(5):126105. https://doi.org/10.1016/j.syapm.2020.126105
Tsuneda S, Miyauchi R, Ohno T, Hirata A (2005) Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene. J Biosci Bioeng 99:403–407. https://doi.org/10.1263/jbb.99.403
Wang S, Zhao J, Ding X, Zhao R, Huang T, Lan L, Naim Bin Nasry AA, Liu S (2019a) Effect of starvation time on NO and N2O production during heterotrophic denitrification with nitrite and glucose shock loading. Process Biochem 86:108–116. https://doi.org/10.1016/j.procbio.2019.07.023
Wang S, Zhao J, Huang T (2019b) High NO and N2O accumulation during nitrite denitrification in lab-scale sequencing batch reactor: influencing factors and mechanism. Environ Sci Pollut Res 26:34377–34387. https://doi.org/10.1007/s11356-019-06391-5
Wang S, Zhao J, Liu S, Zhao R, Hu B (2018) Effect of temperature on nitrogen removal and electricity generation of a dual-chamber microbial fuel cell water. Air Soil Pollut 229:244. https://doi.org/10.1007/s11270-018-3840-z
Wang X, Wang S, Xue T, Li B, Dai X, Peng Y (2015a) Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage. Water Res 77:191–200. https://doi.org/10.1016/j.watres.2015.03.019
Wang Y, Geng J, Guo G, Wang C, Liu S (2011a) N2O production in anaerobic/anoxic denitrifying phosphorus removal process: the effects of carbon sources shock. Chem Eng J 172:999–1007. https://doi.org/10.1016/j.cej.2011.07.014
Wang Y, Geng J, Ren Z, He W, Xing M, Wu M, Chen S (2011b) Effect of anaerobic reaction time on denitrifying phosphorus removal and N2O production. Bioresour Technol 102:5674–5684. https://doi.org/10.1016/j.biortech.2011.02.080
Wang Z, Meng Y, Fan T, Du Y, Tang J, Fan S (2015b) Phosphorus removal and N2O production in anaerobic/anoxic denitrifying phosphorus removal process: long-term impact of influent phosphorus concentration. Bioresour Technol 179:585–594. https://doi.org/10.1016/j.biortech.2014.12.016
Xing W, Zhang W, Li D, Li J, Jia F, Cui Y, Ren F (2017) An integrated O/A two-stage packed-bed reactor (INT-PBR) for total nitrogen removal from low organic carbon wastewater. Chem Eng J 328:894–903. https://doi.org/10.1016/j.cej.2017.07.103
Xu J, Pang H, He J, Nan J (2020) The effect of supporting matrix on sludge granulation under low hydraulic shear force: performance, microbial community dynamics and microorganisms migration. Sci Total Environ 712:136562. https://doi.org/10.1016/j.scitotenv.2020.136562
Yuan C, Wang B, Peng Y, Hu T, Zhang Q, Li X (2020) Nutrient removal and microbial community in a two-stage process: Simultaneous enhanced biological phosphorus removal and semi-nitritation (EBPR-SN) followed by anammox. Bioresour Technol 310:123471. https://doi.org/10.1016/j.biortech.2020.123471
Zeng D, Miao J, Wu G, Zhan X (2018) Nitrogen removal, microbial community and electron transport in an integrated nitrification and denitrification system for ammonium-rich wastewater treatment. Int Biodeter Biodegr 133:202–209. https://doi.org/10.1016/j.ibiod.2018.07.014
Zhang C, Sun G, Zhao K, Zou S, Yuan L (2018) Performance of A2NO-MBR process in treating synthetic and municipal wastewater. Environ Sci Pollut Res 25:10782–10791. https://doi.org/10.1007/s11356-018-1359-x
Zhang M, Zhu C, Pan T, Fan Y, Soares A, Wu J, He C (2020) Nutrient metabolism, mass balance, and microbial structure community in a novel denitrifying phosphorus removal system based on the utilizing rules of acetate and propionate. Chemosphere 257:127076. https://doi.org/10.1016/j.chemosphere.2020.127076
Zhang X, Hua X, Yue X (2017) Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation. Appl Microbiol Biotechnol 101:1697–1707. https://doi.org/10.1007/s00253-016-7949-y
Zhao J, Li Y, Chen X, Li Y (2018) Effects of carbon sources on sludge performance and microbial community for 4-chlorophenol wastewater treatment in sequencing batch reactors. Bioresour Technol 255:22–28. https://doi.org/10.1016/j.biortech.2018.01.106
Zhou Y, Pijuan M, Zeng RJ, Yuan Z (2008) Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge. Environ Sci Technol 42:8260–8265. https://doi.org/10.1021/es800650j
Zhu X, Chen Y (2011) Reduction of N2O and NO generation in anaerobic-aerobic (low dissolved oxygen) biological wastewater treatment process by using sludge alkaline fermentation liquid. Environ Sci Technol 45:2137–2143. https://doi.org/10.1021/es102900h
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This work was supported by the National Natural Science Foundation of China (No. 51778057 and 51808045) and Key Research and Development Program of Ningxia Hui Autonomous Region (No. 2019BFG02031).
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Wang, S., Zhao, J., Ding, X. et al. Nitric oxide and nitrous oxide production in anaerobic/anoxic nitrite-denitrifying phosphorus removal process: effect of phosphorus concentration. Environ Sci Pollut Res 27, 45925–45937 (2020). https://doi.org/10.1007/s11356-020-10499-4
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DOI: https://doi.org/10.1007/s11356-020-10499-4