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Identification and expression analysis of microRNAs in tibial growth plate of chicken through thiram toxicity

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Abstract

Thiram is a widely known tibial dyschondroplasia (TD) inducer. TD, a common metabolic cartilage disease, presents in rapidly growing poultry birds. There are evidences that miRNAs are involved in diverse aspects of normal skeletal development, but very less is known about the role of miRNAs in TD. Therefore, this study aimed to determine which genes and pathways show differential expression between TD suffered chickens and normal chickens. We collected growth plates from ten-days-old TD chickens and control chickens and performed high-throughput RNA sequencing (RNA-Seq). Afterwards, target prediction, GO annotation and KEGG pathway analysis were carried out to understand the role of DEMs (differentially expressed microRNAs). We obtained 96,884,760 and 94,574,290 clean reads and identified 17 significant DEMs between the TD and control groups. Functional enrichment analysis of DEMs indicated that the putative targets of miRNAs were remarkably enriched in bone-related pathways, such as Notch, MAPK and Autophagy. Overall, this study provides detailed understanding about the pathogenesis of thiram induced TD and new insights towards the molecular mechanism of miRNAs.

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Funding

This work was financially supported by the National Natural Science Foundation of China (No. 31873031) and the National Key Research and Development Program of China (Project No. 2017YFD0502200).

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Authors and Affiliations

  1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China

    Zhixing Li, Aoyun Li, Jialu Zhang, Yaping Wang, Hui Zhang, Yi Lian, Mudassar Iqbal & Jiakui Li

  2. University College of Veterinary & Animal Sciences, Islamia University of Bahawalpur 63100, Punja, Pakistan

    Khalid Mehmood & Mudassar Iqbal

  3. College of Animals Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Linzhi, Tibet, 860000, PR China

    Jiakui Li

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  1. Zhixing Li
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  2. Aoyun Li
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Correspondence to Jiakui Li.

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Li, Z., Li, A., Zhang, J. et al. Identification and expression analysis of microRNAs in tibial growth plate of chicken through thiram toxicity. Environ Sci Pollut Res 27, 6628–6636 (2020). https://doi.org/10.1007/s11356-019-06648-z

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  • DOI: https://doi.org/10.1007/s11356-019-06648-z

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