Abstract
Metal-cutting fluids, one of the most consumed materials in the metallurgy industry, turn into oily wastewater after being used in the metalworking processes. The amount of cutting fluids used can reach up to millions of tons. And these invaluable fluids are difficult to distil and expensive, and impossible to store. Even after it is disposed and recaptured, the end product has no commercial value. In this study, the effect of this mixture was examined on the ecosystem using the Allium cepa test system in which onion root tips were treated with three different concentrations of waste-cutting fluid, based on a 24- and 48-h cell cycle. The oily wastewater exhibited a mechanism which triggered the chromosomal and nuclear abnormalities in the onion root-tip meristem and reduced the mitotic index. Common abnormalities observed in the experimental groups based on the water concentration were chromosome stickiness, c-mitosis, and micronuclei formation. In the experimental group with the lowest water concentration, budding nuclei were observed at a different level than all of the other experimental groups. The x-ray fluorescence analysis showed that the concentrations of elements, such as silicon, calcium, iron, and zinc, were higher in the oily wastewater than those in the unused cutting oil.
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Al-Sabti K, Kurelec B (1985) Chromosomal aberration in onion (Allium cepa) induced by water chloration by-products. Bull Envıron Contam Toxicol 34:80–88
Bakare AA, Adeyemi AO, Adeyemi A, Alabi OA, Osibanjo O (2012) Cytogenotoxic effects of electronic waste leachate in Allium cepa. Caryologia 65:94–100
Bartz WJ (1998) Lubricants and the environment. Trıbol Int 31:35–47
Boojar MMA, Goodarzi F (2007) The copper tolerance strategies and the role of antioxidative enzymes in three plant species grown on copper mine. Chemosphere 67:2138–2147
Bortolotto T, Bertoldo JB, Silveira FZ, Defaveri TM, Silvano J, Pich CT (2009) Evaluation of the toxic and genotoxic potential of landfill leachates using bioassays. Environ Toxicol Pharmacol 28:288–293
Chenga C, Phippsa D, Alkhaddarb RM (2005) Treatment of spent metalworking fluids. Water Res 39:4051–4063
Clarens AF, Hayes KF, Skerlos SJ (2006) Feasibility of metalworking fluids delivered in supercritical carbon dioxide. J Manuf Process 8:47–53
Corradi MG, Levi M, Musetti R, Favali MA (1991) The effect of Cr (VI) on different inbred lines of Zea mays, I. Nuclei and cell cycle in the root tip tissue. Protoplasma 162:12–19
D’avino L, Rizzuto G, Guerrini S, Sciaccaluga M, Pagnotta E, Lazzeri L (2015) Environmental implications of crude glycerin used in special productsfor the metalworking industry and in biodegradable mulching films. Ind Crop Prod 75:29–35
Doncheva S, Nicolov B, Ogneva V (1996) Effect of copper excess on the morphology of the nucleus in maize meristem cells. Physiol Plant 96:118–122
Fernandes TCC, Mazzeo DEC, Marin-Morales MA (2007) Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to trifluralin herbicide. Pestic Biochem Physiol 88:252–259
Fernandes TCC, Mazzeo DEC, Marin-Morales MA (2009) Origin of nuclear and chromosomal alterations derived from the action of an aneugenic agent-trifluralin herbicide. Ecotoxicol Environ Saf 72:1680–1686
Firbas P, Amon T (2014) Chromosome damage studies in the onion plant Allium cepa L. Caryologia 67:25–35
Fiskesjö G (1985) The Allium test as a standard in environmental monitoring. Hereditas 102:99–112
Fiskesjö G (1995) Allium test. Methods Mol Biol 43:119–127
Fiskesjö G (2006) The Allium test in wastewater monitoring. Envıron Toxic Water 8:291–298
Fiskesjö G, Levan A (1993) Evaluation of the first ten MEIC chemicals in the Allium cepa test. Atlas 21:139–149
Geremias R, Fattorini D, Fávere VTD, Pedrosa RC (2010) Bioaccumulation and toxic effects of copper in common onion Allium cepa L. J Chem Ecol 26:19–26
Geremias R, De Fávere VT, Rozangela CP, Fattorini D (2011) Bioaccumulation and adverse effects of trace metals and polycyclic aromatic hydrocarbons in the common onion Allium cepa as a model in ecotoxicological bioassays. J Chem Ecol 27:515–522
Glińska S, Bartczak M, Oleksiak S, Wolska A, Gabara B, Posmyk M, Janas K (2007) Effects of anthocyanin-rich extract from red cabbage leaves on meristematic cells of Allium cepa L. roots treated with heavy metals. Ecotoxicol Environ Saf 68:343–350
Gosalia A (2010) The European base oils and lubricants industry: trends in a globalcontext. Lube Mag 95:8–16
Greeley M, Rajagopalan N (2004) Impact of environmental contaminants on machining properties of metalworking fluids. Tribol Int 37:327–332
Leme DM, Marin-Morales MA (2009) Allium cepa test in environmental monitoring: a review on its application. Mutat Res 682:71–81
Leme DM, Angelis DF, Marin-Morales MA (2008) Action mechanisms of petroleum hydrocarbons present in waters impacted by an oil spill on the genetic material of Allium cepa root cells. Aquat Toxicol 88:214–219
Leme DM, Aparecida M, Marin-Morales MA (2009) Allium cepa test in environmental monitoring: a review on its application. Mutat Res 682:71–81
Liu DH, Jiang WS, Li MX (1992) Effects of trivalent and hexavalent chromium on root growth and cell division of Allium cepa. Hereditas 117:23–29
Mazzeo DE, Marin-Morales MA (2015) Genotoxicity evaluation of environmental pollutants using analysis of nucleolar alterations. Environ Sci Pollut Res Int 22:9796–9806
McClure T (2000) New standard performance tests for metal removal fluids. Metal cutting and grinding: technology and market outlook. 2000, Chicago, IL, June 5–7, 2000. Gorham, Maine: Gorham Advanced Materials Inc
Muszynski A, Załeska-Radziwiłł M, Łebkowska M, Nowak D (2007) Biological and electrochemical treatment of used metalworking fluids: a toxicity-reduction evaluation. Arch Environ Contam Toxicol 52:483–488
Pasha MK, Archana A, Shayesta T, Parveen J (2015) Forskolin: genotoxicity assessment in Allium cepa. Mutat Res 777:29–32
Pekol S (2014) Ecotoxicological assessment of metalworking fluids using the Allium cepa test procedure. J Chem Ecol 30:66–75
Pekol S, Caglar A, Aydinli B (2012) The toxic and environmental evaluation of pyrolytic liquids by Allium cepa test. J Chem Ecol 28:65–73
Pettersson A (2007) High-performance base fluids for environmentally adapted lubricants. Tribol Int 40:638–645
Rajeshwari A, Kavitha S, Alex SA, Kumar D, Mukherjee A, Chandrasekaran N, Mukherjee A (2015) Cytotoxicity of aluminum oxide nanoparticles on Allium cepa root tip—effects of oxidative stress generation and biouptake. Environ Sci Pollut Res 22:11057–11066
Rambo CL, Zanotelli P, Dalegrave D, Nez DD, Szczepanik J (2017) Hydropower reservoirs: cytotoxic and genotoxic assessment using the Allium cepa root model. Environ Sci Pollut Res 24:8759–8768
Srivastava R, Kumar D, Gupta SK (2005) Bioremediation of municipal sludge by vermitechnology and toxicity assessment by Allium cepa. Bioresource Technol 96:1867–1871
Teixeira RO, Comparoto ML, Mantovani MS, Vicentini VEP (2003) Assessment of two medicinal plants Psiduim gajava L. and Achulea millefolium L., in vitro and in vivo assays. Genet Mol Biol 226:551–555
Wichmann H, Stache H, Schmidt C, Winter M, Bock R, Herrmann C, Bahadir M (2013) Ecological and economic evaluation of a novel glycerol based biocide-free metalworking fluid. J Clean Prod 43:12–19
Yıldız M, Ciğerci IH, Konuk K, Fidan AF, Terzi H (2009) Determination of genotoxic effects of copper sulphate and cobalt chloride in Allium cepa root cells by chromosome aberration and comet assays. Chemosphere 75:934–938
Zurita JL, Jos A, Peso AD, Salguero M, López-Artíguez M, Repetto G (2007) Ecotoxicological assessment of bromobenzene using a test battery with five model systems. Food Chem Toxıcol 45:575–584
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We would like to extend our thanks to the Kastamonu University Rectorate, the Scientific Research Projects (BAP) Unit, and the Kastamonu University Central Laboratory for their support of this study (KÜBAP-01-2013/24).
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Pekol, S. X-ray fluorescence spectrometry characteristics of oily waste water from steel processing and an evaluation of its impact on the environment. Environ Sci Pollut Res 25, 17100–17108 (2018). https://doi.org/10.1007/s11356-018-1923-4
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DOI: https://doi.org/10.1007/s11356-018-1923-4