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Toxicity against Drosophila melanogaster and antiedematogenic and antimicrobial activities of Alternanthera brasiliana (L.) Kuntze (Amaranthaceae)

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Abstract

Bioactive phytocompounds are studied by several bioactivities demonstrated, as their cytotoxic effects. The aim of this work was to evaluate the phytochemical profile, the toxic effect using the Drosophila melanogaster animal model and the anti-inflammatory and antimicrobial effect of the Alternanthera brasiliana (EEAB) ethanol extract. The phytochemical profile was performed using HPLC. The cytotoxic effect was evaluated in vivo using D. melanogaster. The anti-inflammatory effect was determined by neurogenic and antiedematogenic assays, and the antimicrobial activity was assayed using a microdilution method to determine the minimum inhibitory concentration (MIC) of the EEAB alone and in association with antibiotics. The main compound identified on the EEAB was luteolin (1.93%). Its cytotoxic effect was demonstrated after 24 h in the concentrations of 10, 20 and 40 mg/mL. The extract demonstrated an antiedematogenic effect, with a reduction of the edema between 35.57 and 64.17%. The MIC of the extract was ≥1.024 μg/mL, thus being considered clinically irrelevant. However, when the EEAB was associated with gentamicin, a synergism against all bacterial strains assayed was observed: Staphylococcus aureus (SA10), Escherichia coli (EC06) and Pseudomonas aeruginosa (PA24). Due to these results, the EEAB demonstrated a low toxicity in vivo and anti-inflammatory and synergistic activities. These are promising results, mainly against microbial pathogens, and the compounds identified can be a source of carbon backbones for the discovery and creation of new drugs.

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Authors and Affiliations

  1. Laboratório de Microbiologia e Biologia Molecular, Departamento de Química Biológica, Universidade Regional do Cariri – URCA, Crato, CE, Brazil

    Henrique Douglas Melo Coutinho, Saulo Relison Tintino, Raimundo Luiz Silva Pereira, Thiago Sampaio de Freitas & Maria Ivaneide Rocha

  2. Laboratório de Farmacologia e Química Molecular, Departamento de Química Biológica, Universidade Regional do Cariri – URCA, Crato, CE, Brazil

    Cícera Datiane de Morais Oliveira-Tintino & Irwin Rose Alencar de Menezes

  3. Laboratório de Botânica Aplicada - Departamento de Ciências Biológicas, Universidade Regional do Cariri – URCA, Crato, CE, Brazil

    Maria Arlene Pessoa da Silva

  4. Universidade Federal do Pampa – UNIPAMPA, São Gabriel, RS, Brazil

    Jeferson Luis Franco

  5. Laboratório de Bioprospecção do Semiárido, Departamento de Química Biológica, Universidade Regional do Cariri – URCA, Crato, CE, Brazil

    Francisco Assis Bezerra da Cunha & Joycy Francely Sampaio dos Santos

  6. Laboratório de Pesquisa de produtos Naturais – LPPN, Departamento de Química Biológica, Universidade Regional do Cariri – URCA, Crato, CE, Brazil

    José Galberto Martins da Costa

  7. Universidade Federal de Santa Maria – UFSM, Santa Maria, RS, Brazil

    Aline Augusti Boligon & João Batista Teixeira da Rocha

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  1. Henrique Douglas Melo Coutinho
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Correspondence to Henrique Douglas Melo Coutinho.

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The present study was submitted to the Animal Experimentation and Use Committee (CEUA) of the Regional University of Cariri (URCA) and approved under the protocol number 15/2012.

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Responsible editor: Philippe Garrigues

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Coutinho, H.D.M., de Morais Oliveira-Tintino, C.D., Tintino, S.R. et al. Toxicity against Drosophila melanogaster and antiedematogenic and antimicrobial activities of Alternanthera brasiliana (L.) Kuntze (Amaranthaceae). Environ Sci Pollut Res 25, 10353–10361 (2018). https://doi.org/10.1007/s11356-017-9366-x

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