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Genotype-dependent alleviation effects of exogenous GSH on salinity stress in cotton is related to improvement in chlorophyll content, photosynthetic performance, and leaf/root ultrastructure

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Abstract

Soil salinity is a major abiotic stress that is constraining crop growth and productivity. Greenhouse hydroponic experiments were performed using salt-sensitive (cv. Zhongmian 41) and tolerant (Zhong 9806) cotton seedlings to evaluate how different genotypes responded to salinity stress in the presence of exogenous GSH (reduced glutathione). Cotton plants grown in 150 mM NaCl showed severe reduction in plant height, root length, and shoot and root fresh/dry weight. Salinity also caused reduction in photosynthesis and chlorophyll content, but increase in malondialdehyde (MDA) content. However, the reduction was more in Zhongmian 41 compared to Zhong 9806. Importantly, Sodium concentration was increased in the two genotypes and the induction was more in Zhongmian 41. Calcium and magnesium concentration was decreased in Zhongmian 41; however, in Zhong 9806 there were no significant differences relative to control. Addition of 50 mg L−1 GSH in150 mM NaCl solution (Na + GSH) significantly alleviated salinity stress. Compared with salinity treatment alone (NaCl), Na + GSH increased fresh and dry weight of the root, stem, and leaf, photosynthesis, and chlorophyll content. Obvious ultrastructural alterations were observed in the saline-treated leaf- and root-tip cells. Exogenous GSH greatly ameliorated the salinity-induced damage on the leaf/root ultrastructure, especially in Zhongmian 41.These results advocate a positive role for GSH in alleviation of salinity, which is related to significant improvement in chlorophyll content, photosynthetic performance, and leaf/root ultrastructure.

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Acknowledgements

This work was financially supported by the National R & D Project of Transgenic Crops of Ministry of Science and Technology of China (Major Program, 2009ZXD8001-027B).

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Authors and Affiliations

  1. Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Wasim Ibrahim, Imrul Mosaddek Ahmed, Xianhong Chen & Feibo Wu

  2. Plant Physiology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, 1701, Bangladesh

    Imrul Mosaddek Ahmed

Authors
  1. Wasim Ibrahim
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  2. Imrul Mosaddek Ahmed
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  3. Xianhong Chen
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  4. Feibo Wu
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Corresponding authors

Correspondence to Imrul Mosaddek Ahmed or Feibo Wu.

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Responsible editor: Philippe Garrigues

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Supplemental Table S1

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Supplemental Table S2

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Supplemental Table S3

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Supplemental Fig. S1

Effect of salinity stress and external glutathione supply on MDA content (A, B), (nmol g−1FW) in leaves of Zhongmian 41 (left panel) and Zhong 9806 (right panel) after 5, 10 and 15 d treatment expressed as the percentage of control (%). Error bars represent SD values (n = 5). Different letters indicate significant differences (P < 0.05) among the treatments and refer to each genotype. Control, Na and Na + GSH correspond to basic nutrition solution (BNS), BNS + 150 mM NaCl, and BNS + 150 mM NaCl + 50 μM GSH, respectively. (DOCX 47 kb)

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Ibrahim, W., Ahmed, I.M., Chen, X. et al. Genotype-dependent alleviation effects of exogenous GSH on salinity stress in cotton is related to improvement in chlorophyll content, photosynthetic performance, and leaf/root ultrastructure. Environ Sci Pollut Res 24, 9417–9427 (2017). https://doi.org/10.1007/s11356-017-8611-7

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