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Nitric oxide ameliorates the damaging effects of oxidative stress induced by iron deficiency in cyanobacterium Anabaena 7120

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Abstract

In cyanobacterium Anabaena 7120, iron deficiency leads to oxidative stress with unavoidable consequences. Nitric oxide reduces pigment damage and supported the growth of Anabaena 7120 in iron-deficient conditions. Elevation in nitric oxide accumulation and reduced superoxide radical production justified the role of nitric oxide in alleviating oxidative stress in iron deficiency. Increased activities of antioxidative enzymes and higher levels of ROS scavengers (ascorbate, glutathione and thiol) in iron deficiency were also observed in the presence of nitric oxide. Nitric oxide also supported the membrane integrity of Anabaena cells and reduces protein and DNA damage caused by oxidative stress induced by iron deficiency. Results suggested that nitric oxide alleviates the damaging effects of oxidative stress induced by iron deficiency in cyanobacterium Anabaena 7120.

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Acknowledgments

We are thankful to the Council of Scientific and Industrial Research, New Delhi for providing financial support. The Head, Department of Botany, Banaras Hindu University, Varanasi, India is gratefully acknowledged for providing laboratory facility.

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  1. Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005, India

    Manish Singh Kaushik, Meenakshi Srivastava, Alka Srivastava, Anumeha Singh & Arun Kumar Mishra

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  1. Manish Singh Kaushik
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  2. Meenakshi Srivastava
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  3. Alka Srivastava
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Correspondence to Arun Kumar Mishra.

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Responsible editor: Philippe Garrigues

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Figure S1.

Time dependent exponential decay and production of GSNO and NO under iron deficiency in Anabaena 7120. (GIF 42 kb)

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Kaushik, M.S., Srivastava, M., Srivastava, A. et al. Nitric oxide ameliorates the damaging effects of oxidative stress induced by iron deficiency in cyanobacterium Anabaena 7120. Environ Sci Pollut Res 23, 21805–21821 (2016). https://doi.org/10.1007/s11356-016-7421-7

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