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Nitric Oxide Alleviates Oxidative Damage Induced by Enhanced Ultraviolet-B Radiation in Cyanobacterium

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Abstract

To study the role of nitric oxide (NO) on enhanced ultraviolet-B (UV-B) radiation (280–320 nm)-induced damage of Cyanobacterium, the growth, pigment content, and antioxidative activity of Spirulina platensis-794 cells were investigated under enhanced UV-B radiation and under different chemical treatments with or without UV-B radiation for 6 h. The changes in chlorophyll-a, malondialdehyde content, and biomass confirmed that 0.5 mM sodium nitroprusside (SNP), a donor of nitric oxide (NO), could markedly alleviate the damage caused by enhanced UV-B. Specifically, the biomass and the chlorophyll-a content in S. platensis-794 cells decreased 40% and 42%, respectively under enhanced UV-B stress alone, but they only decreased 10% and 18% in the cells treated with UV-B irradiation and 0.5 mM SNP. Further experiments suggested that NO treatment significantly increased the activities of superoxide dismutase (SOD) and catalase (CAT), and decreased the accumulation of O −2 in enhanced UV-B-irradiated cells. SOD and CAT activity increased 0.95- and 6.73-fold, respectively. The accumulation of reduced glutathione (GSH) increased during treatment with 0.5 mM SNP in normal S. platensis cells, but SNP treatment could inhibit the increase of GSH in enhanced UV-B-stressed S. platensis cells. Thus, these results suggest that NO can strongly alleviate oxidative damage caused by UV-B stress by increasing the activities of SOD, peroxidase, CAT, and the accumulation of GSH, and by eliminating O −2 in S. platensis-794 cells. In addition, the difference of NO origin between plants and cyanobacteria are discussed.

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Acknowledgments

This work was funded by the Projects of Natural Science Foundation of Gansu province (3ZS051-A25-058), Chinese National Nature Science Foundation (30170186), and the open fund of the Ministry of Education Key Laboratory of Arid and Grassland Agroecology, Lanzhou University (Xushijian). We thank Dr. Leah L. Granke and Dr. Ailin Zhou for checking the English. We also thank editors and reviewers for critical comments and suggestions.

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  1. School of Chemistry and Bioengineering, Lanzhou Jiaotong University, Lanzhou, 730070, People’s Republic of China

    Lingui Xue & Shiweng Li

  2. School of Life Sciences, Lanzhou University, State Key Laboratory of Arid Agroecology, Lanzhou, 730000, People’s Republic of China

    Lingui Xue, Shiweng Li, Hongmei Sheng, Huyuan Feng, Shijian Xu & Lizhe An

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  1. Lingui Xue
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Correspondence to Lingui Xue.

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Xue, L., Li, S., Sheng, H. et al. Nitric Oxide Alleviates Oxidative Damage Induced by Enhanced Ultraviolet-B Radiation in Cyanobacterium. Curr Microbiol 55, 294–301 (2007). https://doi.org/10.1007/s00284-006-0621-5

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