Skip to main content
SpringerLink
Log in

Performance of mixed algae for treatment of slaughterhouse wastewater and microbial community analysis

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

This study investigated organic matter (OM) and nutrient removal efficiency of mixed algal species from slaughterhouse wastewater (SWW) by using photo-bioreactor. For this purpose, different dilution multiples of 10, 4, and 2 were applied to the SWW, and pure wastewater was finally used for algal cultivation. OM and nutrient removal performance in an algal photo-bioreactor were severely affected by the dilution ratio. After 7 days of cultivation, the highest removal percentages of total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) were 89.6, 70.2, and 96.2 %, respectively. Furthermore, the changes in eukaryotic algae and cyanobacterial species in the algal photo-bioreactors were investigated using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) techniques. The results indicated that cyanobacterial species were more efficient than eukaryotic species in removing nutrients from the SWW. This study suggests that mixed algal photo-bioreactors could be used efficiently in the treatment of SWW.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Abou-Shanab RA, Matter IA, Kim S-N, Oh Y-K, Choi J, Jeon B-H (2011) Characterization and identification of lipid-producing microalgae species isolated from a freshwater lake. Biomass Bioenergy 35(7):3079–3085

    Article  CAS  Google Scholar 

  • An S, Friedl T, Hegewald E (1999) Phylogenetic relationships of Scenedesmus and Scenedesmus-like coccoid green algae as inferred from ITS-2 rDNA sequence comparisons. Plant Biol 1(4):418–428

    Article  Google Scholar 

  • APHA A WEF (1995) Standard methods for the examination of water and wastewater, 19th edn. American Public Health Association, Washington, DC

    Google Scholar 

  • Aravinthan V, Story N, Yusaf T (2014) Nutrient removal of nursery and municipal wastewater using Chlorella vulgaris microalgae for lipid extraction. Desalin Water Treat 52(4–6):727–736

    Article  CAS  Google Scholar 

  • Ashok V, Shriwastav A, Bose P (2014) Nutrient removal using algal-bacterial mixed culture. Appl Biochem Biotechnol 174(8):2827–2838

    Article  CAS  Google Scholar 

  • Asmare A, Demessie B, Murthy G (2014) Investigation of microalgae co-cultures for nutrient recovery and algal biomass production from dairy manure. Appl Eng Agric 30(2):335–342

    Google Scholar 

  • Cabanelas ITD, Ruiz J, Arbib Z, Chinalia FA, Garrido-Pérez C, Rogalla F, Nascimento IA, Perales JA (2013) Comparing the use of different domestic wastewaters for coupling microalgal production and nutrient removal. Bioresour Technol 131:429–436

    Article  CAS  Google Scholar 

  • Cai T, Park SY, Li Y (2013) Nutrient recovery from wastewater streams by microalgae: status and prospects. Renew Sust Energ Rev 19:360–369

    Article  CAS  Google Scholar 

  • Chan YJ, Chong MF, Law CL, Hassell DG (2009) A review on anaerobic–aerobic treatment of industrial and municipal wastewater. Chem Eng J 155(1-2):1–18

  • Cheng H, Tian G, Liu J (2013) Enhancement of biomass productivity and nutrients removal from pretreated piggery wastewater by mixotrophic cultivation of Desmodesmus sp. CHX1. Desalin Water Treat 51(37–39):7004–7011

    Article  CAS  Google Scholar 

  • Choi HJ, Lee SM (2015) Effect of the N/P ratio on biomass productivity and nutrient removal from municipal wastewater. Bioprocess Biosyst Eng 38(4):761–766

    Article  CAS  Google Scholar 

  • de Mattos LFA, Bastos RG (2015) COD and nitrogen removal from sugarcane vinasse by heterotrophic green algae Desmodesmus sp. Desalin Water Treat 57(10):9465–9473

    Google Scholar 

  • Devi MP, Subhash GV, Mohan SV (2012) Heterotrophic cultivation of mixed microalgae for lipid accumulation and wastewater treatment during sequential growth and starvation phases: effect of nutrient supplementation. Renew Energ 43:276–283

    Article  Google Scholar 

  • Dubey SK, Dubey J, Mehra S, Tiwari P, Bishwas AJ (2011) Potential use of cyanobacterial species in bioremediation of industrial effluents. Afr J Biotechnol 10(7):1125–1132

  • Eland LE, Davenport R, Mota CR (2012) Evaluation of DNA extraction methods for freshwater eukaryotic microalgae. Water Res 46(16):5355–5364

    Article  CAS  Google Scholar 

  • Feng Y, Li C, Zhang D (2011) Lipid production of Chlorella vulgaris cultured in artificial wastewater medium. Bioresour Technol 102(1):101–105

    Article  CAS  Google Scholar 

  • Ferrigo D, Galla G, Sforza E, Morosinotto T, Barcaccia G, Berrini CC (2015) Biochemical characterization and genetic identity of an oil-rich Acutodesmus obliquus isolate. J Appl Phycol 27(1):149–161

    Article  CAS  Google Scholar 

  • Gardner R, Peters P, Peyton B, Cooksey KE (2011) Medium pH and nitrate concentration effects on accumulation of triacylglycerol in two members of the chlorophyta. J Appl Phycol 23(6):1005–1016

    Article  CAS  Google Scholar 

  • Grobbelaar JU (2004) Algal nutrition–mineral nutrition. Handbook of microalgal culture: biotechnology and applied phycology :95–115

  • Hernández D, Riaño B, Coca M, Solana M, Bertucco A, García-González M (2016) Microalgae cultivation in high rate algal ponds using slaughterhouse wastewater for biofuel applications. Chem Eng J 285:449–458

    Article  Google Scholar 

  • Hessen DO, Færøvig PJ, Andersen T (2002) Light, nutrients, and P: C ratios in algae: Grazer performance related to food quality and quantity. Ecology 83(7):1886–1898

  • Ho S-H, Chen C-Y, Yeh K-L, Chen W-M, Lin C-Y, Chang J-S (2010) Characterization of photosynthetic carbon dioxide fixation ability of indigenous Scenedesmus obliquus isolates. Biochem Eng J 53(1):57–62

    Article  CAS  Google Scholar 

  • Hongyang S, Yalei Z, Chunmin Z, Xuefei Z, Jinpeng L (2011) Cultivation of Chlorella pyrenoidosa in soybean processing wastewater. Bioresour Technol 102(21):9884–9890

    Article  Google Scholar 

  • Janse I, Meima M, Kardinaal WEA, Zwart G (2003) High-resolution differentiation of cyanobacteria by using rRNA-internal transcribed spacer denaturing gradient gel electrophoresis. Appl Environ Microbiol 69(11):6634–6643

    Article  CAS  Google Scholar 

  • Ji F, Zhou Y, Pang A, Ning L, Rodgers K, Liu Y, Dong R (2015) Fed-batch cultivation of Desmodesmus sp. in anaerobic digestion wastewater for improved nutrient removal and biodiesel production. Bioresour Technol 184:116–122

    Article  CAS  Google Scholar 

  • Judd S, van den Broeke LJP, Shurair M, Kuti Y, Znad H (2015) Algal remediation of CO2 and nutrient discharges: a review. Water Res 87:356–366

  • Maroneze MM, Barin JS, de Menezes CR, Queiroz MI, Zepka LQ, Jacob-Lopes E (2014) Treatment of cattle-slaughterhouse wastewater and the reuse of sludge for biodiesel production by microalgal heterotrophic bioreactors. Sci Agric 71(6):521–524

  • Merzouki M, Bernet N, Delgenès JP, Benlemlih M (2005) Effect of prefermentation on denitrifying phosphorus removal in slaughterhouse wastewater. Bioresour Technol 96(12):1317–1322

  • Metcalf E (2003) Inc., wastewater engineering, treatment and reuse. McGraw-Hill, New York

    Google Scholar 

  • Muñoz R, Jacinto M, Guieysse B, Mattiasson B (2005) Combined carbon and nitrogen removal from acetonitrile using algal–bacterial bioreactors. Appl Microbiol Biotechnol 67(5):699–707

    Article  Google Scholar 

  • Park K, Whitney C, Kozera C, O’Leary S, McGinn P (2015) Seasonal isolation of microalgae from municipal wastewater for remediation and biofuel applications. J Appl Microbiol 119(1):76–87

    Article  CAS  Google Scholar 

  • Posadas E, García-Encina PA, Domínguez A, Díaz I, Becares E, Blanco S, Munoz R (2014) Enclosed tubular and open algal–bacterial biofilm photobioreactors for carbon and nutrient removal from domestic wastewater. Ecol Eng 67:156–164

    Article  Google Scholar 

  • Ramachandra TV, Madhab MD, Shilpi S, Joshi NV (2013) Algal biofuel from urban wastewater in India: scope and challenges. Renew Sust Energ Rev 21:767–777

  • Selvaratnam T, Pegallapati AK, Montelya F, Rodriguez G, Nirmalakhandan N, Van Voorhies W, Lammers PJ (2014) Evaluation of a thermo-tolerant acidophilic alga, Galdieria sulphuraria, for nutrient removal from urban wastewaters. Bioresour Technol 156:395–399

  • Sevrin-Reyssac J (1998) Biotreatment of swine manure by production of aquatic valuable biomasses. Agric Ecosyst Environ 68(3):177–186

    Article  CAS  Google Scholar 

  • Shen Q-H, Jiang J-W, Chen L-P, Cheng L-H, Xu X-H, Chen H-L (2015) Effect of carbon source on biomass growth and nutrients removal of Scenedesmus obliquus for wastewater advanced treatment and lipid production. Bioresour Technol 190:257–263

    Article  CAS  Google Scholar 

  • Su Y, Mennerich A, Urban B (2012) Comparison of nutrient removal capacity and biomass settleability of four high-potential microalgal species. Bioresour Technol 124:157–162

    Article  CAS  Google Scholar 

  • Taşkan E (2016) Effect of tetracycline antibiotics on performance and microbial community of algal photo-bioreactor. Appl Biochem Biotechnol 1–12. doi:10.1007/s12010-016-2042-7

  • Valta K, Kosanovic T, Malamis D, Moustakas K, Loizidou M (2015) Overview of water usage and wastewater management in the food and beverage industry. Desalin Water Treat 53(12):3335–3347

    Article  CAS  Google Scholar 

  • Vymazal J (2014) Constructed wetlands for treatment of industrial wastewaters: a review. Ecol Eng 73:724–751

    Article  Google Scholar 

  • Wang B, Lan CQ (2011) Biomass production and nitrogen and phosphorus removal by the green alga Neochloris oleoabundans in simulated wastewater and secondary municipal wastewater effluent. Bioresour Technol 102(10):5639–5644

    Article  CAS  Google Scholar 

  • Wang L, Li Y, Chen P, Min M, Chen Y, Zhu J, Ruan RR (2010) Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Bioresour Technol 101(8):2623–2628

    Article  CAS  Google Scholar 

  • Yan C, Zheng Z (2013) Performance of photoperiod and light intensity on biogas upgrade and biogas effluent nutrient reduction by the microalgae Chlorella sp. Bioresour Technol 139:292–299

    Article  CAS  Google Scholar 

  • Yan C, Luo X, Zheng Z (2013a) Performance of purifying anaerobic fermentation slurry using microalgae in response to various LED light wavelengths and intensities. J Chem Technol Biotechnol 88(9):1622–1630

    Article  CAS  Google Scholar 

  • Yan C, Luo X, Zheng Z (2013b) Effects of various LED light qualities and light intensity supply strategies on purification of slurry from anaerobic digestion process by Chlorella vulgaris. Int Biodeterior Biodegrad 79:81–87

    Article  CAS  Google Scholar 

  • Yan C, Zhang L, Luo X, Zheng Z (2013c) Effects of various LED light wavelengths and intensities on the performance of purifying synthetic domestic sewage by microalgae at different influent C/N ratios. Ecol Eng 51:24–32

    Article  Google Scholar 

  • Yan C, Zhao Y, Zheng Z, Luo X (2013d) Effects of various LED light wavelengths and light intensity supply strategies on synthetic high-strength wastewater purification by Chlorella vulgaris. Biodegradation 24(5):721–732

    Article  CAS  Google Scholar 

  • Yan C, Zhang L, Luo X, Zheng Z (2014) Influence of influent methane concentration on biogas upgrading and biogas slurry purification under various LED (light-emitting diode) light wavelengths using Chlorella sp. Energy 69:419–426

    Article  CAS  Google Scholar 

  • Yan C, Muñoz R, Zhu L, Wang Y (2016a) The effects of various LED (light emitting diode) lighting strategies on simultaneous biogas upgrading and biogas slurry nutrient reduction by using of microalgae Chlorella sp. Energy 106:554–561

    Article  CAS  Google Scholar 

  • Yan C, Zhu L, Wang Y (2016b) Photosynthetic CO2 uptake by microalgae for biogas upgrading and simultaneously biogas slurry decontamination by using of microalgae photobioreactor under various light wavelengths, light intensities, and photoperiods. Appl Energy 178:9–18

  • Yang J, Li X, Hu H, Zhang X, Yu Y, Chen Y (2011) Growth and lipid accumulation properties of a freshwater microalga, Chlorella ellipsoidea YJ1, in domestic secondary effluents. Appl Energy 88(10):3295–3299

    Article  CAS  Google Scholar 

  • Yang L, Tan X, Li D, Chu H, Zhou X, Zhang Y, Yu H (2015) Nutrients removal and lipids production by Chlorella pyrenoidosa cultivation using anaerobic digested starch wastewater and alcohol wastewater. Bioresour Technol 181:54–61

    Article  CAS  Google Scholar 

  • Ye Q, Ohtake H, Toda K (1988) Phosphorus removal by pure and mixed cultures of microorganisms. J Ferment Technol 66(2):207–212

    Article  CAS  Google Scholar 

  • Zhang T-Y, Hu H-Y, Wu Y-H, Zhuang L-L, Xu X-Q, Wang X-X, Dao G-H (2016) Promising solutions to solve the bottlenecks in the large-scale cultivation of microalgae for biomass/bioenergy production. Renew Sust Energ Rev 60:1602–1614

    Article  CAS  Google Scholar 

  • Zhu L, Wang Z, Shu Q, Takala J, Hiltunen E, Feng P, Yuan Z (2013) Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment. Water Res 47(13):4294–4302

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Engineering, Department of Environmental Engineering, Firat University, 23119, Elazig, Turkey

    Ergin Taşkan

Authors
  1. Ergin Taşkan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ergin Taşkan.

Additional information

Responsible editor: Gerald Thouand

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Taşkan, E. Performance of mixed algae for treatment of slaughterhouse wastewater and microbial community analysis. Environ Sci Pollut Res 23, 20474–20482 (2016). https://doi.org/10.1007/s11356-016-7241-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-016-7241-9

Keywords

Search

Navigation