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Comparative study of non-invasive methods for assessing Daphnia magna embryo toxicity

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Abstract

Embryos, unlike adults, are typically sessile, which allows for an increase in the available metrics that can be used to assess chemical toxicity. We investigate Daphnia magna development rate and oxygen consumption as toxicity metrics and compare them to arrested embryo development using four different techniques with potassium cyanide (KCN) as a common toxicant. The EC50 (95 % CI) for arrested development was 2,535 (1,747–3,677) μg/L KCN. Using pixel intensity changes, recorded with difference imaging, we semi-quantitatively assessed a decrease in development rate at 200 μg/L KCN, threefold lower than the arrested development lowest observed effect concentration (LOEC). Respirometry and self-referencing (SR) microsensors were two unique techniques used to assess oxygen consumption. Using respirometry, an increase in oxygen consumption was found in the 5 μg/L KCN treatment and a decrease for 148 μg/L, but no change was found for the 78 μg/L KCN treatment. Whereas, with SR microsensors, we were able to detect significant changes in oxygen consumption for all three treatments: 5, 78, and 148 μg/L KCN. While SR offered the highest sensitivity, the respirometry platform developed for this study was much easier to use to measure the same endpoint. Oxygen consumption may be subject to change during the development process, meaning consumption assessment techniques may only be useful only for short-term experiments. Development rate was a more sensitive endpoint though was only reliable four of the six embryonic developmental stages examined. Despite being the least sensitive endpoint, arrested embryo development was the only technique capable of assessing the embryos throughout all developmental stages. In conclusion, each metric has advantages and limitations, but because all are non-invasive, it is possible to use any combination of the three.

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Acknowledgments

The authors would like to acknowledge Eric Karplus from Sciencewares, Inc. for assistance with the DVIT software. This work was supported by the National Science Foundation (CBET- 0854036).

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Authors and Affiliations

  1. Department of Agriculture and Biological Engineering, Purdue University, West Lafayette, IN, USA

    Matthew C. Stensberg, Michael Anthony Zeitchek, Kul Inn & D. Marshall Porterfield

  2. Birck-Bindley Physiological Sensing Facility, Purdue University, West Lafayette, IN, USA

    Matthew C. Stensberg, Michael Anthony Zeitchek, Kul Inn, D. Marshall Porterfield & Maria S. Sepúlveda

  3. Agricultural and Biological Engineering Department, University of Florida, Gainesville, FL, USA

    Eric S. McLamore

  4. Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN, USA

    Maria S. Sepúlveda

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  1. Matthew C. Stensberg
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  2. Michael Anthony Zeitchek
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  3. Kul Inn
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  4. Eric S. McLamore
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  5. D. Marshall Porterfield
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  6. Maria S. Sepúlveda
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Correspondence to Maria S. Sepúlveda.

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Stensberg, M.C., Zeitchek, M.A., Inn, K. et al. Comparative study of non-invasive methods for assessing Daphnia magna embryo toxicity. Environ Sci Pollut Res 21, 10803–10814 (2014). https://doi.org/10.1007/s11356-014-3058-6

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