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Metabolic diversity in tuber tissues of native Chiloé potatoes and commercial cultivars of Solanum tuberosum ssp. tuberosum L.

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Abstract

Introduction

The native potatoes (Solanum tuberosum ssp. tuberosum L.) cultivated on Chiloé Island in southern Chile have great variability in terms of tuber shape, size, color and flavor. These traits have been preserved throughout generations due to the geographical position of Chiloé, as well as the different uses given by local farmers.

Objectives

The present study aimed to investigate the diversity of metabolites in skin and pulp tissues of eleven native accessions of potatoes from Chile, and evaluate the metabolite associations between tuber tissues.

Methods

For a deeper characterization of these accessions, we performed a comprehensive metabolic study in skin and pulp tissues of tubers, 3 months after harvesting. Specific targeted quantification of metabolites using 96 well microplates, and high-performance liquid chromatography combined with non-targeted metabolite profiling by gas chromatography time-of-flight mass spectrometry were used in this study.

Results

We observed differential levels of antioxidant activity and phenolic compounds between skin and pulp compared to a common commercial cultivar (Desireé). In addition, we uncovered considerable metabolite variability between different tuber tissues and between native potatoes. Network correlation analysis revealed different metabolite associations among tuber tissues that indicate distinct associations between primary metabolite and anthocyanin levels, and antioxidant activity in skin and pulp tissues. Moreover, multivariate analysis lead to the grouping of native and commercial cultivars based on metabolites from both skin and pulp tissues.

Conclusions

As well as providing important information to potato producers and breeding programs on the levels of health relevant phytochemicals and other abundant metabolites such as starch, proteins and amino acids, this study highlights the associations of different metabolites in tuber skins and pulp, indicating the need for distinct strategies for metabolic engineering in these tissues. Furthermore, this study shows that native Chilean potato accessions have great potential as a natural source of phytochemicals.

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Acknowledgements

This work was supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) [grant number CBB - AUC-00018-16]. Research fellowships were granted by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to ANN and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) [Grant Number CBB-BPD- 00019-16] to FMOS. ANN, MRD and CIB also acknowledge the support from the Chilean Ministerio de Educación (MEC-CONICYT; Grant PAI80160036). The authors wish to thank the NUBIOMOL-UFV for providing the facilities for the analysis of this work, and Michael Handford (Universidad de Chile) for language support.

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Authors and Affiliations

  1. Núcleo de Investigación en Producción Alimentaría (NIPA-UCT), Universidad Católica de Temuco, P.O. Box 56-D, Temuco, Chile

    Claudio Inostroza-Blancheteau

  2. Departamento de Ciencias Agropecuarias y Acuícolas, Facultad de Recursos Naturales, Universidad Católica de Temuco, P.O. Box 56-D, Temuco, Chile

    Claudio Inostroza-Blancheteau, Fabiola Durán & Jaime Solano

  3. Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-900, Viçosa-Minas Gerais, Brazil

    Franklin Magnum de Oliveira Silva, Mariana Machado & Adriano Nunes-Nesi

  4. Central Metabolism Group, Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam-Golm, Germany

    Toshihiro Obata & Alisdair R. Fernie

  5. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, P.O. Box 54-D, Temuco, Chile

    Marjorie Reyes-Díaz

  6. Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, P.O. Box 54-D, Temuco, Chile

    Marjorie Reyes-Díaz

Authors
  1. Claudio Inostroza-Blancheteau
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  2. Franklin Magnum de Oliveira Silva
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  3. Fabiola Durán
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  4. Jaime Solano
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  5. Toshihiro Obata
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  6. Mariana Machado
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  7. Alisdair R. Fernie
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  8. Marjorie Reyes-Díaz
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  9. Adriano Nunes-Nesi
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Contributions

CIB, MRD, and ANN conceived and designed the research. FD and CIB performed the experiments. TO, MM and FMdeOS contributed powerful analytical tools. CIB, FMdeOS and MM analyzed data. CIB and ANN wrote the manuscript. MRD, TO, JS and ARF revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Claudio Inostroza-Blancheteau or Adriano Nunes-Nesi.

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All authors declare that they have no conflict of interest.

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This article does not contain any studies with human or animal subjects.

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11306_2018_1428_MOESM1_ESM.xlsx

SI Table 1. Antioxidant activity, starch content, total amino acid, total anthocyanin, glucose content, fructose content, sucrose content, total phenol, total flavonoids and total protein in skin and pulp tissues of tubers from 11 native potato (Solanum tuberosum ssp. tuberosum L.) varieties and two commercial cultivars (Desireé and Yagana). Values are mean ± SE (n=5). Values in the same row followed by different letters are statistically different (P < 0.05). FW= fresh weight; DW= dry weight Supplementary material 1 (XLSX 24 KB)

11306_2018_1428_MOESM2_ESM.xlsx

SI Table 2. Relative abundance of primary skin metabolites of 11 native potato (Solanum tuberosum ssp. tuberosum L.) varieties and two commercial cultivars (Desireé and Yagana). Values are mean ± SE (n=5). Values in the same row followed by different letters are statistically different (P < 0.05). Abbreviations: D, Desireé; MGML, Meca de gato morada larga; MA, Michuñe azul; C, Cauchau; G, Guicoña; MN, Michuñe negro; GC, Guadacho colorado; M, Murta; CM, Clavela morada; Y, Yagana; T, Tonta; GB, Guadacho blanco; LV, Lengua de vaca. Supplementary material 2 (XLSX 61 KB)

11306_2018_1428_MOESM3_ESM.xlsx

SI Table 3. Relative abundance of primary pulp metabolites of 11 native potato (Solanum tuberosum ssp. tuberosum L.) varieties and two commercial cultivars (Desireé and Yagana). Values are mean ± SE (n=5). Values in the same row followed by different letters are statistically different (P < 0.05). Abbreviations: D, Desireé; MGML, Meca de gato morada larga; MA, Michuñe azul; C, Cauchau; G, Guicoña; MN, Michuñe negro; GC, Guadacho colorado; M, Murta; CM, Clavela morada; Y, Yagana; T, Tonta; GB, Guadacho blanco; LV, Lengua de vaca. Supplementary material 3 (XLSX 52 KB)

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Inostroza-Blancheteau, C., de Oliveira Silva, F.M., Durán, F. et al. Metabolic diversity in tuber tissues of native Chiloé potatoes and commercial cultivars of Solanum tuberosum ssp. tuberosum L.. Metabolomics 14, 138 (2018). https://doi.org/10.1007/s11306-018-1428-7

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