Abstract
The conservation of genetic diversity is a crucial aspect of forest tree breeding programs, necessitating strategies for its safeguard. Here, the extent of genetic diversity was assessed in 260 Chinese pine (Pinus tabuliformis Carr.) germplasm samples from five provenances using 24 SSR markers. We systematically compared various methods for constructing a core collection aimed at conserving genetic diversity and the results revealed substantial genetic diversity within this germplasm collection. Extensive gene exchange was observed among four of the sampled five provenances which resulted in forming two genetically distinctive groups. To construct the core collection, six different sampling strategies (PowerCore, Power marker_allele number, Power marker_gene entropy, Power marker_gene diversity, Corehunter, and genetic distance-based) and five different sampling sizes (ranging from 10 to 30%) were employed. Comparative analysis of genetic diversity parameters was conducted across the identified 26 subsets, utilizing the PowerCore strategy as the primary approach for capturing all allelic variation present in the core collection, which consisted of only 61 individuals. A supplementary collection of 20 individuals with high genetic variation was identified to provide a final core collection of 81 individuals, representing 31.2% of the initial collection. The constructed core collection effectively captured the genetic diversity present in the initial collection and serves as a valuable resource for preserving genetic richness within the breeding population.
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Data availability
All data related to this paper are accessible through supplemental files, which include the following:
Table S1: Germplasm accession information used in this study.
Table S2: Details of all core germplasm collections and their germplasm accession numbers.
Table S3: Genetic diversity parameters for all core germplasm collections.
Table S4: Original SSR genotyping dataset.
Table S5: Information on the 24 pairs of SSR marker primers utilized in this study.
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This work was financially supported by the National Key R&D Program for the 14th Five-Year Plan in China (2022YFD2200304).
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Yang, B., Wang, H., Xia, Q. et al. Preserving genetic diversity in Pinus tabuliformis breeding population through core collection development. Tree Genetics & Genomes 19, 57 (2023). https://doi.org/10.1007/s11295-023-01631-9
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DOI: https://doi.org/10.1007/s11295-023-01631-9