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Fine mapping of the gene controlling the easy-peeling pellicle trait and development of an efficient marker-assisted selection system in Japanese chestnut (Castanea crenata Sieb. et Zucc.)

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Abstract 

Most Japanese chestnuts have difficult-peeling pellicles, which make it time-consuming and costly to remove the pellicle from the nut during processing. Therefore, an easy-peeling pellicle has been an important goal of Japanese chestnut breeding programs. In previous studies, we identified the peeling locus, which controls peelability in Japanese chestnut. Homozygous recessive plants (p/p) have an easy-peeling pellicle. Here, we developed a novel simple sequence repeat (SSR) marker, CmSca06716, which is suitable for the selection of pellicle peelability in Japanese chestnut. We also constructed a fine map of Tsukuba-43 and localized the peeling locus within a 1.1-cM region between SSR markers CmSca05637 (or CmSca01731) and CmSca03291. The CmSca06716 marker co-segregated with the peeling locus in all F1 plantlets of a ‘Porotan’ × Tsukuba-43 cross. The peeling region was located within a 1.5-Mb region of chromosome 1 in two Chinese chestnut genome assemblies. The 394-bp allele of CmSca06716 was linked in the coupling phase to the recessive easy-peeling allele (p) possessed by ’Porotan’, ’Porosuke’, and ‘Yakko’, which are easy to peel, and in their relatives. We also identified three new cultivars that were heterozygous for the 394-bp allele at the CmSca06716 locus and the p allele at the peeling locus. Furthermore, we developed a cost-efficient and rapid marker-assisted selection system and applied it to our breeding program for Japanese chestnut. This information will lead to a better understanding of the genetic control and physiological mechanisms underlying pellicle peelability in Japanese chestnut.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

The name, version, and parameters of the software used in this study are described in the “Materials and methods” section.

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Acknowledgements

We are grateful to Mss. M. Tsukamoto, H. Takahashi, and N. Minagawa for their technical assistance.

Funding

This work was partially supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics-based Technology for Agricultural Innovation, HOR-2004).

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Authors and Affiliations

  1. Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8605, Japan

    Shingo Terakami, Sogo Nishio, Hidenori Kato, Norio Takada, Yukie Takeuchi, Toshihiro Saito & Toshiya Yamamoto

Authors
  1. Shingo Terakami
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  2. Sogo Nishio
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  3. Hidenori Kato
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  4. Norio Takada
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  5. Yukie Takeuchi
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  6. Toshihiro Saito
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  7. Toshiya Yamamoto
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Corresponding author

Correspondence to Shingo Terakami.

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Data archiving statement

This paper uses existing public data, and there are no new data to register. All of the newly designed primers mapped on linkage map are shown in the table of the manuscript.

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Communicated by A.M. Dandekar.

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Terakami, S., Nishio, S., Kato, H. et al. Fine mapping of the gene controlling the easy-peeling pellicle trait and development of an efficient marker-assisted selection system in Japanese chestnut (Castanea crenata Sieb. et Zucc.). Tree Genetics & Genomes 19, 2 (2023). https://doi.org/10.1007/s11295-022-01575-6

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