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Improvement of the production and quality of Cordyceps javanica conidia for the control of Diaphorina citri adults

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Abstract

This study aimed to evaluate the use of palm kernel meal (PKM) in the traditional solid-state fermentation system to improve the production and quality of Cordyceps javanica conidia. The impact of PKM was determined by measuring conidia yield, viability, hydrophobicity, shelf life, and conidia pathogenicity against Diaphorina citri adults. By supplementing rice grains with 5% palm kernel meal increased the conidial yield by up to 40%, without compromising conidia viability and hydrophobicity. In addition, conidia caused higher levels of mortality by mycosis against D. citri adults (90%), relative to conidia harvested from rice (52%). The conidia recovered from rice/palm kernel meal mixtures also retained viability greater than 90% after storage for 10 months at 4 °C, while the conidia produced on rice reached 80%. Thus, conidia produced in the presence of palm kernel meal can be consumed immediately or in the medium term. Some process advantages of the palm kernel meal as co-substrate in the traditional production system of C. javanica are also mentioned. These results are attractive for improving the mycoinsecticide production process, with excellent cost–benefit and minimal changes in infrastructure and process.

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The data that support the findings of this study are available on request from the corresponding author.

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Acknowledgements

The authors thank the Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCyT) for the scholarships grants provided to AOH (174903), RMM (174904), TPA (204499), OL (20193) and AAC (275592).

Funding

This study was supported by Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA).

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Authors and Affiliations

  1. Universidad de la Cañada, Carretera Teotitlán - San Antonio Nanahuatipán Km 1.7 s/n., Paraje Tiltlacuatitla, Teotitlán de Flores Magón, C.P. 68540, Oaxaca, Mexico

    Armando Ordaz-Hernández

  2. Centro Nacional de Referencia de Control Biológico (CNRF-DGSV-SENASICA), Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico

    Roberto Montesinos-Matías & Marco A. Mellín-Rosas

  3. Tecnológico Nacional de México/TES de Tehuacán, Libramiento Tecnológico S/N, A.P. 247, Santa María Coapan, C.P. 75770, Tehuacán, Puebla, Mexico

    Teresa Pérez-Aguirre

  4. Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

    Octavio Loera

  5. División de Ingeniería Química y Bioquímica, Tecnológico Nacional de México/TES de Ecatepec, Av. Tecnológico s/n, Valle de Anáhuac, sección Fuentes, C.P. 55210, Ecatepec de Morelos, Estado de México, Mexico

    Alejandro Angel-Cuapio

Authors
  1. Armando Ordaz-Hernández
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  2. Roberto Montesinos-Matías
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  3. Marco A. Mellín-Rosas
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  4. Teresa Pérez-Aguirre
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  5. Octavio Loera
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  6. Alejandro Angel-Cuapio
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Contributions

Ordaz-Hernández Armando, Montesinos-Matías Roberto and Mellín-Rosas Marco Antonio: Methodology, Investigation, Supervision, Validation, Writing-Original Draft preparation, Funding acquisition. Teresa Pérez Aguirre, Alejandro Angel-Cuapio and Loera Octavio: Statistical Data Analysis, Writing, Formal analysis, Reviewing and Editing, Conceptualization, Funding acquisition.

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Correspondence to Alejandro Angel-Cuapio.

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Ordaz-Hernández, A., Montesinos-Matías, R., Mellín-Rosas, M.A. et al. Improvement of the production and quality of Cordyceps javanica conidia for the control of Diaphorina citri adults. World J Microbiol Biotechnol 40, 115 (2024). https://doi.org/10.1007/s11274-024-03922-2

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