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Selection of aptamers against pathogenic bacteria and their diagnostics application

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Abstract

Aptamers are short nucleotide sequences which can specifically bind to a variety of targets with high affinity. They are identified and selected via systematic evolution of ligands by exponential enrichment (SELEX). Compared to antibodies, aptamers offer several advantages including easy labeling, high stability and lower cost. Those advantages make it possible to be a potential for use as a recognition probe to replace antibody in the diagnostic field. This article is intended to provide a comprehensive review, which is focused on systemizing recent advancements concerning SELEX procedures, with special emphasis on the key steps in SELEX procedures. The principles of various aptamer-based detections of pathogenic bacteria and their application are discussed in detail, including colorimetric detection, fluorescence detection, electrochemical detection, lateral flow strip test, mass sensitive detection and PCR-based aptasensor. By discussing recent research and future trends based on many excellent publications and reviews, we attempt to give the readers a comprehensive view in the field of aptamer selection against pathogenic bacteria and their diagnostics application. Authors hope that this review will promote lively and valuable discussions in order to generate new ideas and approaches towards the development of aptamer-based methods for application in pathogenic bacteria diagnosis.

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Acknowledgements

This research was supported in part by Open Research Subject of Key Laboratory of Xihua University (szjj2017-115), Walmart Foundation and Walmart Food Safety Collaboration Center. The authors thank Lisa Kelso for English edition and correction.

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Authors and Affiliations

  1. School of Food and Bioengineering, Xihua University, Chengdu, 610039, China

    Lijun Wang

  2. Department of Biological & Agricultural Engineering, University of Arkansas, Fayetteville, AR, 72701, USA

    Lijun Wang, Ronghui Wang & Yanbin Li

  3. Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang, 330047, China

    Hua Wei

  4. Center of Excellence for Poultry Science, University of Arkansas, 203 Engineering Hall, Fayetteville, AR, 72701, USA

    Yanbin Li

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  1. Lijun Wang
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  2. Ronghui Wang
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  4. Yanbin Li
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Correspondence to Yanbin Li.

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Wang, L., Wang, R., Wei, H. et al. Selection of aptamers against pathogenic bacteria and their diagnostics application. World J Microbiol Biotechnol 34, 149 (2018). https://doi.org/10.1007/s11274-018-2528-2

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  • DOI: https://doi.org/10.1007/s11274-018-2528-2

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