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Analysis of the ars gene cluster from highly arsenic-resistant Burkholderia xenovorans LB400

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Abstract

The Burkholderia xenovorans LB400 multireplicon genome displays a relatively high proportion of redundant genes, including several genes predicted to be related to arsenic resistance. These comprise an ars gene cluster, composed of the arsR3, acr3, arsC1 and arsH genes, as well as two arsB, arsC2, and seven individual arsR genes. The objective of this work was to elucidate the involvement of the ars gene cluster in arsenic resistance by the LB400 strain. Susceptibility tests showed that B. xenovorans LB400 is highly resistant to arsenate and arsenite. Arsenic resistance was induced by prior exposure of LB400 to arsenate or arsenite. reverse transcription-polymerase chain reaction assays using total RNA from LB400 showed arsenite-induced transcription of the arsR3 gene, suggesting that the ars gene cluster constitutes an arsenite-responsive operon. Transfer of cloned LB400 ars genes to heterologous Escherichia coli or Pseudomonas aeruginosa strains demonstrated that the ArsR3 transcriptional repressor, ArsC1 arsenate reductase, and the Acr3 arsenite efflux pump encoded in the LB400 ars gene cluster, are all associated to the arsenic resistance phenotype of this strain.

Graphical Abstract

The ars gene cluster from Burkholderia xenovorans LB400 is responsible for the inducible arsenic-resistance phenotype of the bacterium.

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Acknowledgements

This work was supported by grants from Consejo Nacional de Ciencia y Tecnología, México (Conacyt) [Grant No. 220040], and Coordinación de la Investigación Científica, UMSNH [Grant No. 2.6]. NSG and MGSL were recipients of fellowships from Conacyt.

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  1. Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana, Edificio B-3, Ciudad Universitaria, 58030, Morelia, Michoacán, Mexico

    Nallely Serrato-Gamiño, Ma. Guadalupe Salgado-Lora, Martha P. Chávez-Moctezuma, Jesús Campos-García & Carlos Cervantes

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  1. Nallely Serrato-Gamiño
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  2. Ma. Guadalupe Salgado-Lora
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  3. Martha P. Chávez-Moctezuma
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  5. Carlos Cervantes
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Correspondence to Carlos Cervantes.

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Serrato-Gamiño, N., Salgado-Lora, M.G., Chávez-Moctezuma, M.P. et al. Analysis of the ars gene cluster from highly arsenic-resistant Burkholderia xenovorans LB400. World J Microbiol Biotechnol 34, 142 (2018). https://doi.org/10.1007/s11274-018-2526-4

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