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A strain of Meyerozyma guilliermondii isolated from sugarcane juice is able to grow and ferment pentoses in synthetic and bagasse hydrolysate media

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Abstract

The search for new microbial strains that are able to withstand inhibitors released from hemicellulosic hydrolysis and are also still able to convert sugars in ethanol/xylitol is highly desirable. A yeast strain isolated from sugarcane juice and identified as Meyerozyma guilliermondii was evaluated for the ability to grow and ferment pentoses in synthetic media and in sugarcane bagasse hydrolysate. The yeast grew in xylose, arabinose and glucose at the same rate at an initial medium pH of 5.5. At pH 4.5, the yeast grew more slowly in arabinose. There was no sugar exhaustion within 60 h. At higher xylose concentrations with a higher initial cell concentration, sugar was exhausted within 96 h at pH 4.5. An increase of 350 % in biomass was obtained in detoxified hydrolysates, whereas supplementation with 3 g/L yeast extract increased biomass production by approximately 40 %. Ethanol and xylitol were produced more significantly in supplemented hydrolysates regardless of detoxification. Xylose consumption was enhanced in supplemented hydrolysates and arabinose was consumed only when xylose and glucose were no longer available. Supplementation had a greater impact on ethanol yield and productivity than detoxification; however, the product yields obtained in the present study are still much lower when compared to other yeast species in bagasse hydrolysate. By the other hand, the fermentation of both xylose and arabinose and capability of withstanding inhibitors are important characteristics of the strain assayed.

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Acknowledgments

This work was supported financially by Instituto Nacional de Ciência e Tecnologia do Bioetanol—INCT do Bioetanol (FAPESP 2008/57908-6 and CNPq 574002/2008-1) and Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (fellowship grants to C. Martini and C. B. Codato). The authors are greatly indebted to Dr. Ines Conceição Roberto (EEL–USP) for the chromatographic analysis.

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  1. Department of Tecnologia Agroindustrial e Socio-Economia Rural, Universidade Federal de São Carlos – Centro de Ciencias Agrarias, Via Anhanguera, km 174, Araras, SP, 13600-970, Brazil

    Cristina Martini, Carolina Brito Codato, Reinaldo Gaspar Bastos & Sandra Regina Ceccato-Antonini

  2. Centro de Estudos Ambientais, Universidade Estadual Paulista Julio de Mesquita Filho, Avenida 24-A, 1515, Rio Claro, SP, 13506-900, Brazil

    Cristina Martini & Sâmia Maria Tauk-Tornisielo

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  1. Cristina Martini
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  2. Sâmia Maria Tauk-Tornisielo
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  3. Carolina Brito Codato
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  4. Reinaldo Gaspar Bastos
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Correspondence to Sandra Regina Ceccato-Antonini.

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Martini, C., Tauk-Tornisielo, S.M., Codato, C.B. et al. A strain of Meyerozyma guilliermondii isolated from sugarcane juice is able to grow and ferment pentoses in synthetic and bagasse hydrolysate media. World J Microbiol Biotechnol 32, 80 (2016). https://doi.org/10.1007/s11274-016-2036-1

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