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Sources and Persistence of Fecal Indicator Bacteria and Bacteroidales in Sand as Measured by Culture-Based and Culture-Independent Methods: a Case Study at Santa Monica Pier, California

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Abstract

This study investigated causes of persistent fecal indicator bacteria (FIB) in beach sand under the pier in Santa Monica, CA. FIB levels were up to 1000 times higher in sand underneath the pier than that collected from adjacent to the pier, with the highest concentrations under the pier in spring and fall. Escherichia coli (EC) and enterococci (ENT) under the pier were significantly positively correlated with moisture (ρ = 0.61, p < 0.001, n = 59; ρ = 0.43, p < 0.001, n = 59, respectively), and ENT levels measured by qPCR (qENT) were much higher than those measured by membrane filtration (cENT). Microcosm experiments tested the ability of EC, qENT, cENT, and general Bacteroidales (GenBac) to persist under in situ moisture conditions (10 and 0.1%). Decay rates of qENT, cENT, and GenBac were not significantly different from zero at either moisture level, while decay rates for EC were relatively rapid during the microcosm at 10% moisture (k = 0.7 days−1). Gull/pelican marker was detected at 8 of 12 sites and no human-associated markers (TaqHF183 and HumM2) were detected at any site during a 1-day site survey. Results from this study indicate that the high levels of FIB observed likely stem from environmental sources combined with high persistence of FIB under the pier.

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Acknowledgements

We are grateful to Rick Valte and Neal Shapiro for their assistance and to the City of Santa Monica for funding this work. We thank Dr. Amy Zimmer-Faust, Dr. Vanessa Thulsiraj, and Dr. Timothy Riedel for laboratory and field assistance.

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Authors and Affiliations

  1. Department of Civil and Environmental Engineering, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Kathryn B. Mika, Karina A. Chavarria, Greg Imamura, Chay Tang, Robert Torres & Jennifer A. Jay

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  1. Kathryn B. Mika
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  2. Karina A. Chavarria
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  3. Greg Imamura
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  4. Chay Tang
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  5. Robert Torres
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  6. Jennifer A. Jay
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Correspondence to Jennifer A. Jay.

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Highlights

• FIB levels were consistently higher in sand underneath the pier than that collected from adjacent to the pier, showing highest concentrations under the pier in spring and fall.

• EC and ENT were significantly positively correlated with moisture (ρ = 0.61, p < 0.001, n = 59; ρ = 0.43, p < 0.001, n = 59, respectively).

• Minimal decay was observed throughout the course of two microcosms at various moisture levels for ENT and GenBac.

• GenBac was not correlated with either cENT or EC. The strongest correlation was between cENT and EC (r 2 = 0.649, p = 0.002).

• Human markers were not detected, but gull/pelican fecal markers were detected at 8 of 12 sites.

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Mika, K.B., Chavarria, K.A., Imamura, G. et al. Sources and Persistence of Fecal Indicator Bacteria and Bacteroidales in Sand as Measured by Culture-Based and Culture-Independent Methods: a Case Study at Santa Monica Pier, California. Water Air Soil Pollut 228, 124 (2017). https://doi.org/10.1007/s11270-017-3291-y

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  • DOI: https://doi.org/10.1007/s11270-017-3291-y

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