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Unique RNA 2 sequences of two Brazilian isolates of Pepper ringspot virus, a tobravirus

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Abstract

Pepper ringspot virus (PepRSV) is a tobravirus reported only in Brazil. Here, the sequences of the complete RNA 2 segments and the 3′ end of the RNA 1 genomic regions of two new isolates from tomato plants were analyzed. The main ORF encodes the CP gene as other tobraviruses and termed ORF 1 of RNA 2. The second ORF was found only in one of the new isolates, although this gene was absent in the type isolate, CAM (collected in the 1960’s). Interestingly, this ORF 2 gene did not show any nucleotide and amino acid sequence similarities with known 2b genes of tobraviruses, an essential gene of tobraviruses for nematodes-transmission. The 5′UTR sequence of RNA 2 segment of CAM isolate was previously reported showing two impaired direct repeats; however, the direct-repeats were absent in these new isolates. An additional ORF was predicted upstream of the CP gene. This putative protein possessed a transmembrane domain similar to the ORFN1 of RNA 2 of Tobacco rattle virus SYM isolate, although there was no sequence similarity. This is the first report on the diversity of the RNA 2 sequences of PepRSV.

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Acknowledgments

The work was funded by “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq, Brazil) for the DSc scholarship to the first, third, and sixth authors. The second, fourth, seventh, and eighth authors are research fellows of CNPq.

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Authors and Affiliations

  1. Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil

    Adriana Ribeiro Silva Batista, Kelly Barreto Rodrigues & Tatsuya Nagata

  2. Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil

    Cícero Nicolini, Fernando Lucas Melo, Raquel Medeiros Vasques & Tatsuya Nagata

  3. Embrapa Vegetables, Brasília, DF, Brazil

    Mônica Alves de Macêdo & Alice Kazuko Inoue-Nagata

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  1. Adriana Ribeiro Silva Batista
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  2. Cícero Nicolini
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  3. Kelly Barreto Rodrigues
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  4. Fernando Lucas Melo
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  5. Raquel Medeiros Vasques
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  6. Mônica Alves de Macêdo
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  7. Alice Kazuko Inoue-Nagata
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  8. Tatsuya Nagata
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Correspondence to Tatsuya Nagata.

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Batista, A.R.S., Nicolini, C., Rodrigues, K.B. et al. Unique RNA 2 sequences of two Brazilian isolates of Pepper ringspot virus, a tobravirus. Virus Genes 49, 169–173 (2014). https://doi.org/10.1007/s11262-014-1066-8

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  • DOI: https://doi.org/10.1007/s11262-014-1066-8

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