Abstract
This study evaluated the effects of a multi-strain prebiotics and probiotics on the diet of tambaqui Colossoma macropomum. One hundred and fifty juvenile tambaqui (20.2 ± 3.6 g and 10.32 ± 5.78 cm) were randomly distributed in 15 experimental units with a volume of 80 L and fed for 45 days with a diet containing the symbiotic additive at five inclusion levels (0, 2, 4, 6 and 8 g kg feed− 1). At the end of the period, growth performance, survival, hemato-biochemical and intestinal parameters, as well as the fish’s resistance to stress were evaluated. The additive did not alter the growth performance, hemato-biochemical and intestinal parameters of the tambaqui. However, the hemato-biochemical parameters of aspartate aminotransferase (AST), plasma protein, hematocrit, glucose, triglycerides and cholesterol showed differences between treatments after the stress challenge. AST increased significantly during challenge and post-challenge. Plasma protein increased significantly during and after the challenge. The hematocrit was highest at 48 h after the challenge and lowest in the 2 g, 6 g and 8 g groups. Glucose was significantly reduced 24 h after the challenge, while triglycerides were lower 24 h and 48 h after the challenge. Cholesterol increased significantly in the challenge. There was an interaction between the factors sampling time and symbiotic concentration for hematocrit and glucose. In hypoxic stress situations, the 2 g concentration was more favorable for the fish. We recommend further studies with 2 g kg feed− 1 in trials lasting more than 45 days.
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Acknowledgements
In memory of Professor Edilson Rodrigues Matos, we would like to thank for his valuable contribution to this research and on Amazon fish parasitology. The authors would like to thank the PROCAD Amazônia for financial support to Professor Nuno F. A. Correia de Melo, 40221741291, public notice n° 21/2018, project nº 88887.200588/2018-00.
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Rafael José Furtado Souza: Experimental execution, Manuscript writing. Edilson Rodrigues Matos: Experimental execution. Aldry Lorran da Silva Souza: Experimental execution. Paola Fabiana Fazzi-Gomes: Experimental execution, analysis. Nuno Filipe Alves Correia de Melo: Hematological analysis. Marco Shizuo Owatari: Data curation, Manuscript review and final writing. Glauber David Almeida Palheta: Methodology, Validation, Investigation. Rodrigo Takata: Methodology, Validation, Investigation, Data curation, Visualization. Fabio Carneiro Sterzelecki: Experimental execution, Data curation, Manuscript review, Supervision.
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This research was approved by the Ethics Committee on Animal Use (CEUA) of the Federal Rural University of Amazon - UFRA, Belem, Brazil, protocol number 9354190820. The Juvenile tambaqui used in the study were purchased from a commercial fish farm located in Rondônia - Brazil and remained at the Sustainable Aquaculture Laboratory at UFRA for 10 days to acclimatize to the experimental conditions. All fish used in biological analyses were previously anesthetized with Eugenol (65 mg L– 1) (Roubach et al. 2005) and euthanized by spinal cord transection.
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Souza, R.J.F., Matos, E.R., da Silva Souza, A.L. et al. Dietary effect of multi-strain prebiotics and probiotics on growth, hemato-biochemical parameters, intestinal histomorphometry, and resistance to hypoxia in juvenile tambaqui (Colossoma macropomum). Vet Res Commun 48, 1061–1072 (2024). https://doi.org/10.1007/s11259-023-10279-8
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DOI: https://doi.org/10.1007/s11259-023-10279-8