Abstract
The most ubiquitously diagnosed microsporidian species in animals and humans is Enterocytozoon bieneusi (E. bieneusi). In this case, this work aimed to probe the occurrence and genotypes of this pathogen in captive alpine musk deer (Moschus chrysogaster) in Gansu Province, China. After fecal sample collection (n = 201) from three farms in Gansu, the internal transcribed spacer (ITS) region of the rRNA gene was probed by PCR for detection and genotyping of E. bieneusi. The infection rate of E. bieneusi in alpine musk deer was 6.0% (12/201), with 0% (0/36), 7.7% (5/65) and 7.0% (7/100) in farms 1 to 3, respectively. The infection rate of E. bieneusi in young alpine musk deer (3.2%; 1/31) is lower than that of adults (6.5%; 11/170), with no evident significant differences between age groups (P > 0.05). Three known genotypes D (n = 8), EbpA (n = 3) and BEB6 (n = 1) were identified by sequence analysis. This is the first such scrutiny of E. bieneusi infection in alpine musk deer in China as per our knowledge. Genotypes D and EbpA were common in humans and animals that is suggestive of the plausible zoonotic role in E. bieneusi transmission by alpine musk deer.
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This work was supported by the National Natural Science Foundation of China (31860699), and the Program for Young and Middle-aged Leading Science, Technology, and Innovation of Xinjiang Production & Construction Corps (2018CB034).
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MQ and WZ contributed to the study conceive and design. QW and AL contributed to acquisition of sample. AL performed experiments. HT, YL and FY contributed to statistical analysis. WZ contributed to writing the manuscript. MQ obtained funding. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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No experimental animals were involved in this study. This study was unsuitable for the approbation by the ethics committee. Prior to sample collection, requisite permissions were obtained from all farmers. No animals were harmed during sampling.
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Zhao, A., Wang, Q., Tuo, H. et al. Molecular detection and characterization of Enterocytozoon bieneusi in captive alpine musk deer (Moschus chrysogaster) in Central and Eastern of Gansu Province, China. Vet Res Commun 46, 1343–1348 (2022). https://doi.org/10.1007/s11259-022-09992-7
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DOI: https://doi.org/10.1007/s11259-022-09992-7