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Sp1 is overexpressed and associated with progression and poor prognosis in bladder urothelial carcinoma patients

  • Urology - Original Paper
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Abstract

Background

Specificity protein 1 (Sp1) is a transcription factor that exerts key functions in the carcinogenesis and progression of various types of cancer. However, its expression and prognostic value in bladder urothelial carcinoma (BUC) have yet to be completely elucidated.

Methods

The present study performed reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to examine Sp1 mRNA expression in 12 pairs of urothelial carcinoma and adjacent normal bladder tissues. Immunohistochemistry (IHC) was performed in 113 paraffin-embedded urothelial carcinoma tissues to detect the expression of Sp1. Kaplan–Meier plots and Cox proportional hazards regression model were used to analyze the correlation between Sp1 expression and patient prognosis.

Results

The mRNA expression of Sp1 was elevated in the urothelial carcinoma by RT-qPCR compared with their paired normal bladder tissues. Among 113 cases of patients with urothelial carcinoma, there were 39 low histological grade and 74 high histological grade, 61 unifocal tumor and 52 multifocal tumor, 78 cases in Ta, T1, and T2 stages, and 35 cases in T3 and T4 stages. The enhanced expression of Sp1 mRNA was observed in tumors with a high histological grade, and invasive and metastatic samples. Immunohistochemistry revealed that Sp1 high expression was significantly correlated with the histological grade, tumor stage, vascular invasion, lymph node metastasis and distant metastasis (P < 0.05). Kaplan–Meier analysis demonstrated that elevated Sp1 expression in cancer tissue was correlated with a significantly poor overall survival (OS) and disease-free survival (DFS) compared with samples with low Sp1 expression (P < 0.05). Multivariate analyses by Cox’s proportional hazard model also revealed that the expression of Sp1 was an independent prognostic factor in urothelial carcinoma.

Conclusion

Sp1 expression is significantly elevated in urothelial carcinoma and may be used to identify a subset of patients with aggressive behaviors and poor clinical outcomes. Sp1 is a potential novel independent prognostic biomarker for patients with urothelial carcinoma following surgery.

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Authors and Affiliations

  1. Department of Urology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No. 150 Ximen Street, Taizhou, 317000, Zhejiang Province, People’s Republic of China

    Jialiang Zhu, Ziwen Lu, Mang Ke & Xianguo Cai

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  1. Jialiang Zhu
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  2. Ziwen Lu
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  3. Mang Ke
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  4. Xianguo Cai
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Contributions

JZ analyzed the data, wrote the manuscript and performed the experiment of immunohistochemistry; MK performed the experiment and analyzed the data; ZL analyzed the data; CG designed the study and revised the manuscript.

Corresponding author

Correspondence to Xianguo Cai.

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All authors declare that there are no competing financial interests.

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Zhu, J., Lu, Z., Ke, M. et al. Sp1 is overexpressed and associated with progression and poor prognosis in bladder urothelial carcinoma patients. Int Urol Nephrol 54, 1505–1512 (2022). https://doi.org/10.1007/s11255-022-03212-6

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