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RETRACTED ARTICLE: Morphological study of chronic prostatitis–chronic pelvic pain syndrome (CP/CPPS) normal modeling dose of T2 peptide in mice

  • Urology - Original Paper
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This article was retracted on 12 September 2023

This article has been updated

Abstract

Objectives

However, the pathogenesis and etiology of CP/CPPS are still poorly understood. Therefore, there is a need for further research through the Image J software to develop models capable of imitating the pathogenesis and etiology of CP/CPPS with different doses of the pathogenesis and the etiology of CP/CPPS is still poorly understood. The aim was to determine the area of the prostatic interstitium, the localization of the inflammation, and the impact of different doses on the group model.

Materials and methods

A total of 30 male ICR mice were randomly grouped into 5 (n = 6): 45 μg group = 6, 60  μg group = 6, 90  μg group = 6, 120  μg group = 6, 120  μg group = 6, control group = 6. With the exception of the control group, all the groups were immunized by injecting 0.2 mL of T2 peptide emulsion and immune adjuvant CFA to induce non-bacterial chronic prostatitis on days 0 and 14 of the mice and finally executed on day 28. All injections were administered subcutaneously. HE staining was used to evaluate changes in prostate pathology. Image J was used to calculate the area of the prostate interstitium, which represents the degree of prostate edema. To compare statistical differences between groups, the ANOVA test was used.

Results

From the perspective of pathological scoring, the 60 μg, 90  μg, and 120  μg groups had the highest scores using Image J to treat inflammatory cells. In addition, in the prostate interstitium area treated, it was found that the 90  μg group attained the largest prostate interstitial area as well as the highest degree of swelling.

Conclusions

From the results, Image J software is an effective tool in the calculating the surface of the prostatic interstitium and the specific localization of the inflammation.

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References

  1. Roberts RO et al (1998) Prevalence of a physician-assigned diagnosis of prostatitis: the Olmsted County Study of Urinary Symptoms and Health Status Among Men. Urology 51(4):578–584

    Article  CAS  PubMed  Google Scholar 

  2. Collins MM et al (1998) How common is prostatitis? A national survey of physician visits. J Urol 159(4):1224–1228

    Article  CAS  PubMed  Google Scholar 

  3. Habermacher GM, Chason JT, Schaeffer AJ (2006) Prostatitis/chronic pelvic pain syndrome. Annu Rev Med 57:195–206

    Article  CAS  PubMed  Google Scholar 

  4. Wenninger K et al (1996) Sickness impact of chronic nonbacterial prostatitis and its correlates. J Urol 155(3):965–968

    Article  CAS  PubMed  Google Scholar 

  5. Thakkinstian A et al (2012) α-blockers, antibiotics and anti-inflammatories have a role in the management of chronic prostatitis/chronic pelvic pain syndrome. BJU Int 110(7):1014–1022

    Article  CAS  PubMed  Google Scholar 

  6. Benson PJ, Smith CS (1992) Cytomegalovirus prostatitis. Urology 40(2):165–167

    Article  CAS  PubMed  Google Scholar 

  7. Ku JH, Paick JS, Kim SW (2005) Chronic prostatitis in Korea: a nationwide postal survey of practicing urologists in 2004. Asian J Androl 7(4):427–432

    Article  PubMed  Google Scholar 

  8. Schaeffer AJ et al (2003) Summary consensus statement: diagnosis and management of chronic prostatitis/chronic pelvic pain syndrome. Eur Urol Suppl 2(2):1–4

    Article  Google Scholar 

  9. Tyagi P et al (2015) Mp25-07 prostatic inflammation evokes upregulation of neurotrophins in sensory ganglia: possible contribution to dysfunctional voiding. J Urol 193(4S):e287–e287

    Article  Google Scholar 

  10. Ludwig M et al (2001) Immunocytological analysis of leukocyte subpopulations in urine specimens before and after prostatic massage. Eur Urol 39(3):277–282

    Article  CAS  PubMed  Google Scholar 

  11. Batstone G, Doble A, Gaston J (2002) Autoimmune T cell responses to seminal plasma in chronic pelvic pain syndrome (CPPS). Clin Exp Immunol 128(2):302–307

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Kouiavskaia DV et al (2009) T-cell recognition of prostatic peptides in men with chronic prostatitis/chronic pelvic pain syndrome. J Urol 182(5):2483–2489

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Wang W et al (2018) Experimental rodent models of chronic prostatitis and evaluation criteria. Biomed Pharmacother 108:1894–1901

    Article  PubMed  Google Scholar 

  14. Khan FU et al (2017) A novel mouse model of chronic prostatitis/chronic pelvic pain syndrome induced by immunization of special peptide fragment with aluminum hydroxide adjuvant. Immunol Lett 187:61–67

    Article  PubMed  Google Scholar 

  15. Murphy SF, Schaeffer AJ, Thumbikat P (2014) Immune mediators of chronic pelvic pain syndrome. Nat Rev Urol 11(5):259

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Tang M et al (2019) Effect of oral T2 antigen on chronic prostatitis/chronic pelvic pain syndrome in mice model. Inflammation 42(6):2086–2094

    Article  CAS  PubMed  Google Scholar 

  17. Cheng Y et al (2019) Novel treatment of experimental autoimmune prostatitis by nanoparticle-conjugated autoantigen peptide T2. Inflammation 42(3):1071–1081

    Article  CAS  PubMed  Google Scholar 

  18. Wang W et al (2019) Morphological reseach on expression of inflammatory mediators in murine models of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by T2 antigen. Andrologia 51:11

    Article  Google Scholar 

  19. Cao Y et al (2019) A nanoparticle-coupled T2 peptide induces immune tolerance and ameliorates chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) in mice model. Fund Clin Pharmacol 33(3):267–276

    Article  CAS  Google Scholar 

  20. Wang W et al (2019) Morphological reseach on expression of inflammatory mediators in murine models of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by T2 antigen. Andrologia 51(11):e13435

    Article  PubMed  Google Scholar 

  21. Keetch DW, Humphrey P, Ratliff TL (1994) Development of a mouse model for nonbacterial prostatitis. J Urol 152(1):247–250

    Article  CAS  PubMed  Google Scholar 

  22. Rivero VE et al (1998) Non-obese diabetic (NOD) mice are genetically susceptible to experimental autoimmune prostatitis (EAP). J Autoimmun 11(6):603–610

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are very thankful to Pr. Daorong Wang and Pr. Xiaohui Zhou countless support and contribution to our research work.

Funding

This work was supported by the National Natural Science Foundation of China (No. 8197102014).

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, Northern Jiangsu People’s Hospital Affiliated to Yangzhou University, No.98 Nantong West Road, Yangzhou, 225001, Jiangsu Province, China

    Maladho Tanta Diallo, Muhammad Ali, Qi Zhang, Yang Wang, Ziyang Long, Jianyue Ding, Cangyuan Zhang, Xiaodong Zhu, Qiannan Sun & Daorong Wang

  2. General Surgery Institute of Yangzhou, Yangzhou University, Yangzhou, 225001, China

    Maladho Tanta Diallo, Muhammad Ali, Qi Zhang, Yang Wang, Ziyang Long, Jianyue Ding, Cangyuan Zhang, Xiaodong Zhu, Mingrui Jiang, Qiannan Sun & Daorong Wang

  3. Clinical Medical College of Yangzhou University, Yangzhou, 225001, China

    Maladho Tanta Diallo, Muhammad Ali, Qi Zhang, Yang Wang, Ziyang Long, Jianyue Ding, Cangyuan Zhang, Xiaodong Zhu & Mingrui Jiang

  4. Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, School of Pharmacy, China Pharmaceutical University, 639# Longmian Avenue, Nanjing, Jiangsu Province, 211198, People’s Republic of China

    Maladho Tanta Diallo & Xiaohui Zhou

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  1. Maladho Tanta Diallo
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  2. Muhammad Ali
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  3. Qi Zhang
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  7. Cangyuan Zhang
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  9. Mingrui Jiang
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  10. Qiannan Sun
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  11. Xiaohui Zhou
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Contributions

MTD performed the research and wrote the paper, DW and XZ designed the research study, MA, QZ, YW, ZL, JD, CZ, XZ, MJ, and QS help with data analysis. All authors read and approved the manuscript for submission.

Corresponding author

Correspondence to Daorong Wang.

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All authors declare no conflict of interest.

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This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1007/s11255-023-03788-7

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Diallo, M.T., Ali, M., Zhang, Q. et al. RETRACTED ARTICLE: Morphological study of chronic prostatitis–chronic pelvic pain syndrome (CP/CPPS) normal modeling dose of T2 peptide in mice. Int Urol Nephrol 54, 1199–1206 (2022). https://doi.org/10.1007/s11255-022-03185-6

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  • DOI: https://doi.org/10.1007/s11255-022-03185-6

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