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Folic acid and flaxseed oil supplements in Ossimi ewes: effect on body weight changes, progesterone profile, blood chemistry, and litter traits

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Abstract

The aim was to explore the impact of periconceptional folic acid or flaxseed oil (FXO) supplementation on fertility, progesterone profile, and blood chemistry in pregnant ewes during the breeding season. In total, 54 Ossimi ewes were divided into three groups (18 animals each). The control treatment (CON) fed a basal diet only, while the others fed the basal diet and supplemented every other day with a single bolus of folic acid (FO 500 μg/head) or flaxseed oil (FX 50 ml/head). During the early stage of pregnancy, the FO and FX groups showed significantly higher serum antioxidant activity (glutathione and superoxide dismutase) as compared with the control CON group (P = 0.012 and 0.007, respectively). Although no significant variations were detected in the serum nitric oxide levels during the early stage and mid-stage of pregnancy, the FO and FX groups showed significantly lower serum nitric oxide concentration in the late stage of pregnancy (P = 0.001). The FO and FX groups showed significantly higher serum progesterone concentrations during the early stage (10.9 and 11.4 ng/ml, respectively) and mid-stage (22.2 and 23.4 ng/ml, respectively) of pregnancy as compared with the CON group (7.72 and 13.9 ng/ml, respectively). The FX group exhibited a significantly higher lambing rate (P = 0.034), as well as the proportion of female lambs (P = 0.029) as compared with the CON group. In conclusion, supplementing Ossimi ewes with folic acid or FXO significantly improved the progesterone profile during pregnancy. Moreover, the FXO supplementation significantly increased the lambing rate and the female lamb rate as compared with the control group.

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References

  • Aebi, H., 1984. Catalase in vitro. Methods in enzymology, 105,121–126.

    Article  CAS  PubMed  Google Scholar 

  • Ambrose, D.J., Kastelic, J.P., Corbett, R., Pitney, P.A., Petit, H.V., Small, J.A., Zalkovic, P., 2006. Lower pregnancy losses in lactating dairy cows fed a diet enriched in α-linolenic acid. Journal of Dairy Science, 89, 3066–3074.

    Article  CAS  PubMed  Google Scholar 

  • AOAC., 1990. Official Methods of Analysis. 15th Edition, Association of Official Analytical Chemists, Washington DC.

  • Asgari safdar, A., Daghigh Kia, H., Moghaddam, G., Khani, A., Alijani, S., 2013. Reproductive Performance and Blood Metabolites Concentration in Iranian Afshari Ewes Fed Calcium Salts of Fatty Acids (CSFA) in Flushing Period, International Journal of Advanced Biological and Biomedical Research, 1, 669–676.

    Google Scholar 

  • Case, A.P., Royle, M., Scheuerle, A.E., Carmichael, S.L., Moffitt, K., Ramadhani, T., 2014. Birth defects, causal attributions, and ethnicity in the National Birth Defects Prevention Study. Journal of Genetic Counseling, 23, 860–873.

    Article  PubMed  Google Scholar 

  • Clayton, E.H., Friend, M.A., Wilkins, J.F., 2016. Increasing the proportion of female lambs by feeding Merino ewes a diet high in omega-6 fatty acids around mating. Animal Production Science 56, 1174–1184.

    Article  CAS  Google Scholar 

  • Collins, T.F., Sprando, R.L., Black, T.N., Olejnik, N., Wiesenfeld, P.W., Babu, U.S., Bryant, M., Flynn, T.J., Ruggles, D.I., 2003. Effects of flaxseed and defatted flaxseed meal on reproduction and development in rats. Food and chemical toxicology, 41, 819–834.

    Article  CAS  PubMed  Google Scholar 

  • Czeizel, A.E., Vargha, P., 2004. Periconceptional folic acid/multivitamin supplementation and twin pregnancy. American journal of obstetrics and gynecology, 191, 790–794.

    Article  CAS  PubMed  Google Scholar 

  • Derrick, B., Dobson-Mckittrick, A., Toher, D., White, P., 2015. Test statistics for comparing two proportions with partially overlapping samples. Journal of Applied Quantitative Methods, 10(3).

    Google Scholar 

  • Đidara, M., Poljičak-Milas, N., Milinković-Tur, S., Mašek, T., Šuran, J., Pavić, M., Kardum, M., Šperanda, M., 2015. Immune and oxidative response to linseed in the diet of periparturient Holstein cows. Animal, 9,1349–1354.

    Article  PubMed  CAS  Google Scholar 

  • Dirandeh, E., Towhidi, A., Zeinoaldini, S., Ganjkhanlou, M., Ansari Pirsaraei, Z., Fouladi-Nashta, A., 2013a. Effects of different polyunsaturated fatty acid supplementations during the postpartum periods of early lactating dairy cows on milk yield, metabolic responses, and reproductive performances. J. Anim. Sci, 91, 713–721.

    Article  CAS  PubMed  Google Scholar 

  • Dirandeh, E., Towhidi A., Ansari Pirsaraei Z., Adib Hashemi F., Ganjkhanlou M., Zeinoaldini S., Rezaei Roodbari A., Saberifar T., Petit H.V., 2013b. Plasma concentrations of PGFM and uterine and ovarian responses in early lactation dairy cows fed omega-3 and omega-6 fatty acids. Theriogenology, 80, 131–137.

    Article  CAS  PubMed  Google Scholar 

  • Dumlao, D.S., Buczynski, M.W., Norris, P.C., Harkewicz, R., Dennis, E.A., 2011. High-throughput lipidomic analysis of fatty acid derived eicosanoids and N-cylethanolamines. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids, 1811, 724–736.

    Article  CAS  Google Scholar 

  • Duplessis, M., Girard, C.L., Santschi, D.E., Laforest, J.P., Durocher, J., Pellerin, D., 2014. Effects of folic acid and vitamin B12 supplementation on culling rate, diseases, and reproduction in commercial dairy herds. Journal of dairy science, 97, 2346–2354.

    Article  CAS  PubMed  Google Scholar 

  • El-Tarabany, A.A., Teama, F.E., Atta, M.A., El-Tarabany, M.S., 2018. Impact of dietary fenugreek seeds on lactational performance and blood biochemical and hematological parameters of dairy goats under hot summer conditions. Mljekarstvo, 68, 214–223.

    Article  CAS  Google Scholar 

  • Fuentes, M.C., Calsamiglia, S., Sanchez, C., Gonzalez, A., Newbold, J.R., Santos, J.E.P., Rodriguez-Alcala, L.M., Fontecha, J., 2008. Effect of extruded linseed on productive and reproductive performance of lactating dairy cows. Livestock Science, 113, 144–154.

    Article  Google Scholar 

  • Godfrey, R.W., Weis, A.J., Dodson, R.E., 2003. Effect of flushing hair sheep ewes during the dry and wet seasons in the U. S. Virgin Islands. J. Anim. Vet. Adv, 2, 184–190.

    Google Scholar 

  • Gong, Z., Ambrosone, C.B., McCann, S.E., Zirpoli, G., Chandran, U., Hong, C.C., Bovbjerg, D.H., Jandorf, L., Ciupak, G., Pawlish, K., Lu, Q., 2014. Associations of dietary folate, Vitamins B6 and B12 and methionine intake with risk of breast cancer among African American and European American women. International journal of cancer, 134, 1422–1435.

    Article  CAS  PubMed  Google Scholar 

  • Gonthier, C., Mustafa A.F., Ouellet D.R., Chouinard P.Y., Berthiaume R., Petit H.V., 2005. Feeding micronized and extrudedflaxseed to dairy cows: Effects on blood parameters and milk fatty acid composition. J. Dairy Sci, 88, 748–756.

    Article  CAS  PubMed  Google Scholar 

  • Gulliver, C.E., Friend, M.A., King, B.J., Wilkins, J.F., Clayton, E.H., 2013. A higher proportion of female lambs when ewes were fed oats and cottonseed meal prior to and following conception. Animal Production Science, 53, 464–471.

    Article  CAS  Google Scholar 

  • Habibzadeh, N., Schorah, C.J., Smithells, R.W., 1986. The effects of maternal folic acid and vitamin C nutrition in early pregnancy on reproductive performance in the guinea-pig. Br. J. Nutr, 55, 23–35.

    Article  CAS  PubMed  Google Scholar 

  • Harper, A.F., Knight, J.W., Kokue, E., Usry, J.L., 2003. Plasma reduced folates, reproductive performance, and conceptus development in sows in response to supplementation with oxidizedand reduced sources of folic acid. Journal of animal science, 81, 735–744.

    Article  CAS  PubMed  Google Scholar 

  • Hashem, N.M., El-Zarkouny, S.Z., Taha, T.A., Abo-Elezz, Z.R., 2011. Effect of season, month of parturition and lactation on estrous behavior and ovarian activity in Barki Rahmani crossbred ewes under subtropical conditions. Theriogenology, 75, 1327–1335.

    Article  CAS  PubMed  Google Scholar 

  • Heravi, M.A., Gilbert, R., Overton, T., Bauman, D., Butler, W., 2007. Effects of feeding fish meal and n-3 fatty acids on ovarian and uterine responses in early lactating dairy cows. J. Dairy Sci, 90, 145–154.

    Article  Google Scholar 

  • Jayatilleke, E., Shaw, S., 1993. A high performance liquid chromatographic assay for reduced and oxidized glutathione in biological samples. Anal. Biochem, 214, 452–457.

    Article  CAS  PubMed  Google Scholar 

  • karalas, F., Karatepe, M., Baysar, A., 2002. Determination of free malondialdehyde in human serum by high performance liquid chromatography. Anal. Biochem, 311, 76–79.

    Article  CAS  Google Scholar 

  • Kholif, A.E., Morsy, T.A., Abdo, M.M., 2018. Crushed flaxseed versus flaxseed oil in the diets of Nubian goats: Effect on feed intake, digestion, ruminal fermentation, blood chemistry, milk production, milk composition and milk fatty acid profile, Animal Feed Science and Technology, 244, 66–75.

    Article  CAS  Google Scholar 

  • Koyuncu, M., Canbolat, O., 2009. Effect of different dietary energy levels on the reproductive performance of Kivircik sheep under a semi-intensive system in the South-Marmara region of Turkey. J. Anim. Feed Sci, 18, 620–627.

    Article  Google Scholar 

  • Lee, S.J., Kang, M.H., Min, H., 2011. Folic acid supplementation reduces oxidative stress and hepatic toxicity in rats treated chronically with ethanol. Nutrition research and practice, 5, 520–526.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lewinska, A., Zebrowski, J., Duda, M., Gorka, A., Wnuk, M., 2015. Fatty acid profile and biological activities of linseed and rapeseed oils. Molecules, 20, 22872–22880.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li, H.Q., Liu, Q., Wang, C., Yang, Z.M., Guo, G., Huo, W.J., Pei, C.X., Zhang, Y.L., Zhang, S.L., Wang, H., Liu, J.X., 2016. Effects of dietary supplements of rumen-protected folic acid on lactation performance, energy balance, blood parameters and reproductive performance in dairy cows. Animal Feed Science and Technology, 213, 55–63.

    Article  CAS  Google Scholar 

  • Lindemann, M.D., 1993. Supplemental folic acid: a requirement for optimizing swine reproduction. J. Anim. Sci, 71, 239–246.

    Article  CAS  PubMed  Google Scholar 

  • Lluís, L., Taltavull, N., Muñoz-Cortés, M., Sánchez-Martos, V., Romeu, M., Giralt, M., Molinar-Toribio, E., Torres, J.L., Pérez-Jiménez, J., Pazos, M., Méndez, L., 2013. Protective effect of the omega-3 polyunsaturated fatty acids: Eicosapentaenoic acid/Docosahexaenoic acid 1:1 ratio on cardiovascular disease risk markers in rats. Lipids in health and disease, 12, 140.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Mahla, A.S., Chaudhari, R.K., Verma, A.K., Singh, A.K., Singh, S.K., Singh, G., Sarkar, M., Dutta, N., Kumar, H., Krishnaswamy, N., 2017. Effect of dietary supplementation of omega-3 polyunsaturated fatty acid (PUFA) rich fish oil on reproductive performance of the goat (Capra hircus). Theriogenology, 99, 79–89.

    Article  CAS  PubMed  Google Scholar 

  • Marei, W.F., Khalil, W.A., Pushpakumara, A.P., El-Harairy, M.A., El-Atta, A.M.A., Wathes, D.C., Fouladi-Nashta, A., 2018. Polyunsaturated fatty acids influence offspring sex ratio in cows. International Journal of Veterinary Science and Medicine, 6, S36-S40.

    Article  PubMed  PubMed Central  Google Scholar 

  • Martin, C., Rouel, J., Jouany, J.P., Doreau, M., Chilliard, Y., 2008. Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed or lionseed oil. J. Anim. Sci, 86, 2642–2650.

    Article  CAS  PubMed  Google Scholar 

  • Mattos, R., Staples, C.R., Thatcher, W.W., 2000. Effects of dietary fatty acids on reproduction in ruminants. Rev Reprod, 5, 38–45.

    Article  CAS  PubMed  Google Scholar 

  • Methot, H., Girard, C.L., Matte, J.J., Castonguay, F.W., 2008. Effects of dietary supplements of folic acid on reproductive performance in ewes. Can. J. Anim. Sci, 88, 489–497.

    Article  CAS  Google Scholar 

  • Moallem, U., 2009. The effects of extruded flaxeed supplementation to high-yielding dairy cows on milk production and milk fatty acid composition. Anim. Feed Sci. Technol, 152, 232–242.

    Article  CAS  Google Scholar 

  • Nandi, A., Chatterjee, I., 1988. Assay of superoxide dismutase activity in animal tissues. Journal of Biosciences,13, 305–315.

    Article  CAS  Google Scholar 

  • Nazir, G., Ghuman, S.P.S., Singh, J., Honparkhe, M., Ahuja, C.S., Dhaliwal, G.S., Sangha, M.K., Saijpaul, S., Agarwal, S.K., 2013. Improvement of conception rate in postpartum flaxseed supplemented buffalo with Ovsynch+ CIDR protocol. Animal reproduction science, 137, 15–22.

    Article  CAS  PubMed  Google Scholar 

  • NRC, 2007. Nutrient Requirements of Small Ruminants. Natl. Acad. of Sci., Washington, DC.

    Google Scholar 

  • Ocak, N., Cam, M.A., Kuran, M., 2006. The influence of pre- and post-mating protein supplementation on reproductive performance in ewes maintained on rangeland. Small Ruminant Res, 64, 16–21.

    Article  Google Scholar 

  • Ojeda, M.L., Rua, R.M., Nogales, F., Díaz-Castro, J., Murillo, M.L., Carreras, O., 2015. The benefits of administering folic acid in order to combat the oxidative damage caused by binge drinking in adolescent rats. Alcohol and Alcoholism, 51, 235–241.

    Article  PubMed  CAS  Google Scholar 

  • Sarath, T., Suguna K., Mehrotra S., Agarwal S.K., Sastry K.V.H., Shanker U., 2010. Serum nitric oxide profile in cyclic, acyclic and pregnant goats. The Indian veterinary journal, 87, 881–883.

    Google Scholar 

  • Sen, U., Sirin, E., Kuran, M., 2013. The effect of maternal nutritional status during mid-gestation on placental characteristics in ewes. Anim. Reprod. Sci, 137, 31–36.

    Article  CAS  PubMed  Google Scholar 

  • Tagbo, I.F., Hill, D.C., 1977.Effects of folic acid deficiency on pregnant rats and their offspring. Can. J. Physiol. Pharmacol, 55, 427–433.

    Article  CAS  PubMed  Google Scholar 

  • Tremblay, G.F., Matte, J.J., Dufour, J.J., Brisson, G.J., 1989. Survival rate and development of fetuses during the first 30 days of gestation after folic acid addition to a swine diet. J. Anim. Sci, 67, 724–732.

    Article  CAS  PubMed  Google Scholar 

  • Warn, L, Webb Ware, J., Salmon, L., Donnelly, J.B., Alcock, D., 2006. ‘Analysis of the profitability of sheep wool and meat enterprises in southern Australia.’ (Sheep Co-operative Research Centre: Armidale, NSW).

  • Ying, S., Wang, Z., Wang, C., Nie, H., He, D., Jia, R., Wu, Y., Wan, Y., Zhou, Z., Yan, Y.,Zhang, Y., Wang, F., 2011. Effect of different levels of short-term feed intake on folliculogenesis and follicular fluid and plasma concentrations of lactate dehydrogenase, glucose, and hormones in Hu sheep during the luteal phase. Reproduction, 142, 699–710.

    Article  CAS  PubMed  Google Scholar 

  • Zeron, Y., Sklan, D., Arav, A., 2002. Effect of polyunsaturated fatty acid supplementation on biophysical parameters and chilling sensitivity of ewe oocytes. Molecular Reproduction and Development, 61, 271–278.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, El-Zeraa Str. 114, Zagazig, Sharkia, 44511, Egypt

    Mahmoud S. El-Tarabany

  2. Biological Applications Department, Radioisotopes Applications Division, NRC, Atomic Energy Authority, Inshas, Cairo, Egypt

    Mostafa A. Atta, Sana S. Emara & Mohamed M. Mostafa

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  1. Mahmoud S. El-Tarabany
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  2. Mostafa A. Atta
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Correspondence to Mahmoud S. El-Tarabany.

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El-Tarabany, M.S., Atta, M.A., Emara, S.S. et al. Folic acid and flaxseed oil supplements in Ossimi ewes: effect on body weight changes, progesterone profile, blood chemistry, and litter traits. Trop Anim Health Prod 52, 301–308 (2020). https://doi.org/10.1007/s11250-019-02017-7

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