Abstract
To address the major challenge of controlling flowering in roses, we studied the ectopic expression of a FLOWERING LOCUS T (FT) homolog, which plays an important role in flowering time regulation in plants. We detected seven rose genes of the phosphatidyl ethanolamine-binding protein (PEBP) family and selected one of them (RoFT) based on its co-localization with flowering and architecture QTLs to study its overexpression in two rose genotypes. Embryogenic lines were obtained and genetic transformation was performed on the rose genotypes. Regenerated plantlets were maintained in a greenhouse until flowering and then floral and architectural traits were scored. We observed substantial variation in phenotypic expression between genotypes and between events, especially in the flowering date and number of floral organs. The expression of 14 genes putatively involved in floral initiation (mainly FT targets) and floral development (from the class ABC model) was studied to gain insight into these variations. We noted than target genes are up- or down-regulation and can partially explain the observed phenotype. Our results revealed that a gene ectopically overexpressed in different genetic backgrounds could have diverse effects and that the overexpression RoFT gene can have pleiotropic effects.
Key message
The overexpression of RoFT gene in two rose varieties induces pleotropic effects and up- or down-regulates several target genes.
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Acknowledgements
The authors thank Christine Boursier and Rémi Gardet and the IRHS-Phenotic (https://doi.org/10.17180/YKBZ-2V85) team for plant management in the greenhouse. We acknowledge the Biological Resource Center (Pome Fruits and Roses, Angers, France) for managing the rose genetic resources and the students Colyne Angelin, Helene Duck, Alexandre Rouinsard, Corentin Chateau, Safae Ouchetto and Wassim Ouchetto for their in vitro culture technical assistance. We are also thankful to Fabienne Simmoneau and Aurelia Roland (IMAC from SFR Quasav) for their help with the microscopic analyses. We acknowledge David Manley for proofreading the English in this manuscript.
This project was funded by the French National Research Agency (ANR) (Program ANR-11-BTBR-001-GENIUS).
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FF, LH, LHSO conceived and managed this study and wrote this manuscript. LH, LV, JB, SA, SF managed in vitro plants, conducted plant genetic transformation. JC, TT conducted data collecting and managed the plants into the greenhouse. LO and JJ cloned RoFT for Agrobacterium tumefaciens genetic transformation. JB, LO conducted qRT-PCR experiments.
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Hamama, L., Bosselut, J., Voisine, L. et al. Rose FT homologous gene overexpression affects flowering and vegetative development behavior in two different rose genotypes. Plant Cell Tiss Organ Cult 156, 87 (2024). https://doi.org/10.1007/s11240-024-02695-8
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DOI: https://doi.org/10.1007/s11240-024-02695-8