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A protocol for rapid in vitro propagation of genetically diverse pitaya

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Abstract

An efficient propagation system of pitaya with diverse genetic background was established by using tender stem as explants. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for shoot proliferation. Zeatin (ZT), thidiazuron (TDZ), 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (6-BA) alone could induce multiple shoots. In that case, the most shoots per explant i.e. 4.6 (13.68 µM ZT), 4.8 (0.11 µM TDZ), 4.3 (17.76 µM 6-BA) and 3.2 (0.23 µM 2, 4-D) were obtained. The best PGRs combination was MS medium with 3.0 µM ZT and 0.5 µM IBA producing the most shoots per explant and the most vigorous shoots. Of the two varieties and six superior selections cultured on the best PGRs combination, more than 6.0 shoots per explant were obtained. No polymorphism was detected among in vitro-derived plantlets selected at random after 11 sub-cultures. Those results showed that MS media with 13.68 µM ZT and 2.46 µM IBA was suitable for shoot propagation of diverse pitaya varieties and selections. The protocol can be used for large-scale propagation of pitaya to meet the demand of increasing commercial cultivation.

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Acknowledgments

This work was supported by Science and Technology Planning Project of Guangzhou (2014Y2-00164); Agricultural Technology Extension Project of Guangdong Province (5300-F13085); Key Laboratory of Innovation and Utilization for Germplasm Resources in Horticultural Crops in South China of Guangdong Higher Education Institutes, South China Agricultural University (SCAU) (KBL11008); Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, South China of Ministry of Agriculture, College of Horticulture, SCAU.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China

    Qingzhu Hua, Pengkun Chen, Wanqing Liu, Yuewen Ma, Ruiwei Liang, Lu Wang, Zehuai Wang, Guibing Hu & Yonghua Qin

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  1. Qingzhu Hua
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  2. Pengkun Chen
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  3. Wanqing Liu
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  4. Yuewen Ma
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  5. Ruiwei Liang
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  6. Lu Wang
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  7. Zehuai Wang
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  8. Guibing Hu
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  9. Yonghua Qin
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Corresponding authors

Correspondence to Guibing Hu or Yonghua Qin.

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Qing-zhu Hua, Pengkun Chen and Wanqing Liu have contributed equally to this work.

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Hua, Q., Chen, P., Liu, W. et al. A protocol for rapid in vitro propagation of genetically diverse pitaya. Plant Cell Tiss Organ Cult 120, 741–745 (2015). https://doi.org/10.1007/s11240-014-0643-9

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