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Impact of a novel precursor on FK506 production and key gene transcription in Streptomyces tsukubaensis No. 9993

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Abstract

FK506, a widely used immunosuppressant, is produced by industrial fermentation using Streptomyces tsukubaensis. In this study, a novel precursor, salicyl alcohol, was added and provided significant improvement in FK506 production. Furthermore, differences of the expression of the structural genes between the two media were also investigated. The results showed that the expression of key genes in enriched medium was much higher than that in the control. Compared with the non-enriched medium, the enriched medium caused 52.25-% enhancement of FK506 production. Salicyl alcohol could play a crucial role in the transcription of FK506 structural genes.

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  1. Department of Bioengineering, East China University of Science and Technology, Shanghai, China

    Yan Sun & Ruifang Ye

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  1. Yan Sun
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  2. Ruifang Ye
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Correspondence to Ruifang Ye.

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Sun, Y., Ye, R. Impact of a novel precursor on FK506 production and key gene transcription in Streptomyces tsukubaensis No. 9993. Res Chem Intermed 42, 3351–3358 (2016). https://doi.org/10.1007/s11164-015-2215-y

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  • DOI: https://doi.org/10.1007/s11164-015-2215-y

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