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Transferability of Microsatellites from Psidium guajava to Eugenia, Myrciaria, Campomanesia, and Syzygium Species (Myrtaceae)

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Abstract

Myrtaceae has many fruit-bearing species of economic and ecological relevance worldwide, but with microsatellite molecular markers (simple sequence repeats, SSR) available for only few of them. Thus, the transferability of SSR is advantageous for characterization, conservation, and breeding of these species. In this work, 158 SSR from Psidium guajava were evaluated for transferability in 18 fruit trees of the genera Eugenia, Campomanesia, Myrciaria, and Syzygium. Of those, 152 SSR transferred to at least 1 and up to 16 species; 47 SSR amplified in more than 10 species, the most conserved; and 13 SSR transferred to 1 or 2 species, demonstrating specificity. A large number of conserved SSR are also present in the genetic map of P. guajava, and may be helpful for synteny and comparative genomic studies. The average of transferability was lower for Syzygium and similar in the other genera. Transferability in the species varied from 20 SSR for Syzygium cumini to 91 for Myrciaria sp.2, being higher on average in the genus Eugenia. Clustering based on the pattern of transferability between species revealed two groups, one composed by Eugenia and Campomanesia and another by Syzygium and Myrciaria, which indicates conserved microsatellite regions within and between genera. This study also allowed selection of the most suitable SSR for each species and genus by amplification quality and study type, thus contributing for a better comprehension of the relationships between the species, taxonomy, structure and genetic diversity, construction of genetic maps, and breeding and conservation programs in the family.

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References

  • Acuña CV, Fernandez P, Villalba PV, García MN, Hopp HE, Poltri SNM (2012) Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus. Tree Genet Genomes 8:289–301

    Article  Google Scholar 

  • Ahmed TA, Al-Qaradawi AY (2009) Molecular phylogeny of Qatari date palm genotypes using simple sequence repeats markers. Biotechnology 8:126–131

    Article  CAS  Google Scholar 

  • Barbará T, Palma-Silva C, Paggi GM, Bered F, Fay MF, Lexer C (2007) Cross-species transfer of nuclear microsatellite markers: potential and limitations. Mol Ecol 16:3759–3767

    Article  PubMed  Google Scholar 

  • Barroso GM (1991) Myrtaceae. In: Sistemática de angiospermas do Brasil. UFV, Viçosa-MG, 2:114-126

  • Barthe S, Gureli F, Barkley NA, Maggia L, Cardi C, Scotti I (2012) Always look on both sides: phylogenetic information conveyed by simple sequence repeat allele sequences. PloS One 7:1–9

    Article  Google Scholar 

  • Biffin E, Lucas EJ, Craven LA, da Costa IR, Harrington MG, Crisp MD (2010) Evolution of exceptional species richness among lineages of fleshy-fruited Myrtaceae. Ann Bot 106:79–93

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Brondani RPV, Brondani C, Tarchini R, Grattapaglia D (1998) Development, characterization and mapping of microsatellite markers in Eucalyptus grandis and E. urophylla. Theor Appl Genet 97:816–827

    Article  CAS  Google Scholar 

  • Brondani RPV, Williams ER, Brondani C, Grattapaglia D (2006) A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus. BMC Plant Biol 6:1–16

    Article  Google Scholar 

  • Buschiazzo E, Gemmell NJ (2009) Evolutionary and phylogenetic significance of platypus microsatellites conserved in mammalian and other vertebrate genomes. Aust J Zool 57:175–184

    Article  Google Scholar 

  • Costa IR (2004) Estudos cromossômicos em espécies de Myrtaceae Juss. no sudeste do Brasil. Dissertation, University State of Campinas

  • Curtu AL, Finkeldey R, Gailing O (2004) Comparative sequencing of a microsatellite locus reveals size homoplasy within and between European oak species (Quercus ssp.). Plant Mol Biol Report 22:339–346

    Article  CAS  Google Scholar 

  • Dickey AM, Hall PM, Shatters Júnior RG, Mckenzie CL (2013) Evolution and homoplasy at the Bem6 microsatellite locus in three sweetpotato whitefly (Bemisia tabaci) cryptic species. BMC Res Notes 6:249

    Article  PubMed Central  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • England PR, Usher AV, Whelan RJ, Ayre DJ (2002) Microsatellite diversity and genetic structure of fragmented populations of the rare, fire-dependent shrub Grevillea macleayana. Mol Ecol 11:967–977

    Article  CAS  PubMed  Google Scholar 

  • Ferreira-Ramos R, Accoroni KAG, Rossi A, Guidugli MC, Mestriner MA, Martinez CA, Alzate-Marin AL (2014) Genetic diversity assessment for Eugenia uniflora L., E. pyriformis Cambess, E. brasiliensis Lam. and E. francavilleana O. Berg neotropical tree species (Myrtaceae) with heterologous SSR markers. Genet Resour Crop Evol 61:267–272

    Article  Google Scholar 

  • Govaerts R, Sobral M, Ashton P, Barrie F, Holst BK, Landrum LR, Matsumoto K, Mazine FF, Nic Lughadha E, Proença CEB, Silva LHS, Wilson PG, Lucas EJ (2008) World checklist of Myrtaceae. Kew Publishing, Royal Botanic Gardens

    Google Scholar 

  • Grattapaglia D, Vaillancourt RE, Shepherd M, Thumma BR, Foley W, Külheim C, Potts BM, Myburg AA (2012) Progress in Myrtaceae genetics and genomics: Eucalyptus as the pivotal genus. Tree Genet Genomes 8:463–508

    Article  Google Scholar 

  • Guavamap, Generation and screening of microsatellite markers (SSRs) in Guava (2008). http://www.neiker.net/neiker/guavamap/for1-6a.html. Accessed 24 April 2013

  • Guo W, Wang W, Zhou B, Zhang W (2006) Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Theor Appl Genet 112:1573–1581

    Article  CAS  PubMed  Google Scholar 

  • Guyomarc H, Sourdille P, Charmet G, Edwards KJ, Bernard M (2002) Characterization of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172

    Article  Google Scholar 

  • He X, Wang Y, Li F, Weng Q, Li M, Xu LA, Gan S (2012) Development of 198 novel EST-derived microsatellites in Eucalyptus (Myrtaceae). Am J Bot 99:134–148

    Article  Google Scholar 

  • Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK (2011) Microsatellite markers: an overview of the recent progress in plants. Euphytica 177:309–334

    Article  CAS  Google Scholar 

  • Lucas EJ, Harris SA, Mazine FF, Belsham SR, Lughadha EMN, Telford A, Gasson PE, Chase MW (2007) Suprageneric phylogenetics of Myrteae, the generically richest tribe in Myrtaceae (Myrtales). Taxon 4:1105–1128

    Article  Google Scholar 

  • Oliveira EJ, Pádua JG, Zucchi MI, Vencovsky R, Vieira MLC (2006) Origin, evolution and genome distribution of microsatellites. Genet Mol Biol 29:294–307

    Article  CAS  Google Scholar 

  • Ottewell KM, Donnellan SC, Moran GF, Paton DC (2005) Multiplexed microsatellite markers for the genetic analysis of Eucalyptus leucoxylon (Myrtaceae) and their utility for ecological and breeding studies in other Eucalyptus Species. J Hered 96:445–451

    Article  CAS  PubMed  Google Scholar 

  • Parida SK, Kalia SK, Sunita K, Dalal V, Hemaprabha G, Selvi A, Pandit A, Singh A, Gaikwad K, Sharma TR, Srivastava PS, Singh NK, Mohapatra T (2009) Informative genomic microsatellite markers for efficient genotyping applications in sugarcane. Theor Appl Genet 118:327–338

    Article  CAS  PubMed  Google Scholar 

  • Rai MK, Phulwaria M, Shekhawat NS (2013) Transferability of simple sequence repeat (SSR) markers developed in guava (Psidium guajava L.) to four Myrtaceae species. Mol Biol Rep 40:5067–5071

    Article  CAS  PubMed  Google Scholar 

  • Ramos R, Ravest G, Méndez MA, Hinrichsen P (2012) Highly polymorphic microsatellite markers for Ugni molinae Turcz., and endemic shrub harboring edible berries from the Southern Cone of South America. Mol Ecol Resour 12:1–8

    Article  Google Scholar 

  • Risterucci AM, Duval MF, Rohde W, Billotte N (2005) Isolation and characterization of microsatellite loci from Psidium guajava L. Mol Ecol Notes 5:745–748

    Article  CAS  Google Scholar 

  • Rodriguez H, Geistlinger J, Berlyn G, Kahl G, Weising K (2000) Characterization of novel microsatellite loci isolated from the tropical dioecious tree Simarouba amara. Mol Ecol 9:489–504

    Article  Google Scholar 

  • Santos KL, Welter LJ, Dantas ACM, Guerra MP, Ducroquet JPJ, Nodari RO (2007) Transference of microsatellite markers from Eucalyptus spp to Acca sellowiana and the successful use of this technique in genetic characterization. Genet Mol Biol 30:73–79

    Article  Google Scholar 

  • Santos KL, Ducroquet JPHJ, Nodari RO (2011) Caracterização genética de populações naturais de goiabeira serrana (Acca sellowiana) com marcadores microssatélites heterólogos. Biotemas 4:75–83

    Google Scholar 

  • Shepherd M, Kasem S, Lee D, Henry R (2006) Construction of microsatellite linkage maps for Corymbia. Silvae Genet 55:228–238

    Google Scholar 

  • Sonah H, Deshmukh RK, Sharma A, Singh VP, Gupta DK, Gacche RN, Rana JC, Singh NK, Sharma TR (2011) Genome-wide distribution and organization of microsatellites in plants: an insight into marker development in Brachypodium. PloS One 6:21298

    Article  Google Scholar 

  • Stàgel A, Portis E, Toppino GL, Rotino LS (2008) Gene-based microsatellite development for mapping and phylogeny studies in eggplant. BMC Genomics 9:1–14

    Article  Google Scholar 

  • Steane D, Vaillancourt R, Russell J, Powell W, Marshall D, Potts B (2001) Development and characterisation of microsatellite loci in Eucalyptus globule (Myrtaceae). Silvae Genet 50:89–91

    Google Scholar 

  • Suwabe K, Iketani H, Numone T, Kage T, Hirai M (2002) Isolation and characterization of microsatellites in Brassica rapa L. Theor Appl Genet 104:1092–1098

    Article  CAS  PubMed  Google Scholar 

  • Swapna M, Sivaraju K, Sharma RK, Singh NK, Mohapatra T (2011) Single-strand conformational polymorphism of EST-SSRs: a potential tool for diversity analysis and varietal identification in sugarcane. Plant Mol Biol Report 29:505–513

    Article  Google Scholar 

  • Taylor DR, Keller SR (2007) Historical range expansion determines the phylogenetic diversity introduced during contemporary species invasion. Evolution 61:335–345

    Google Scholar 

  • Varshney RK, Graner A, Sorrells ME (2005) Genic microsatellite markers in plants: features and applications. Trends Biotechnol 23:48–55

    Article  CAS  PubMed  Google Scholar 

  • Wilson PG, O’Brien M, Gadek PA, Quinn CJ (2001) Myrtaceae revisited: a reassessment of intrafamilial groups. Am J Bot 11:2013–2025

    Article  Google Scholar 

  • Wilson PG, O’Brien MM, Heslewood MM, Quinn CJ (2005) Relationships within Myrtaceae sensu lato based on a matK phylogeny. Plant Syst Evol 251:3–19

    Article  Google Scholar 

  • Zucchi MI, Brondani RPV, Pinheiro JB, Brondani C, Vencovsky R (2002) Transferability of microsatellite markers from Eucalyptus spp. to Eugenia dysenterica (Myrtaceae family). Mol Ecol Notes 2:512–513

    Article  CAS  Google Scholar 

  • Zucchi MI, Brondani RPV, Pinheiro JB, Chaves LJ, Coelho ASG, Vencovsky R (2003) Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. Genet Mol Biol 26:449–457

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil)), the Foundation for Research Support of Espírito Santo (Fundação de Amparo à Pesquisa do Espírito Santo (FAPES, Vitória, ES, Brazil)), and the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil)) for financial support.

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  1. Genetics and Plant Breeding Laboratory, Department of Biology, Center for Agricultural Sciences, Federal University of Espírito Santo, 29.500-000, Alegre, ES, Brazil

    Angélica Maria Nogueira, Adésio Ferreira & Marcia Flores da Silva Ferreira

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  1. Angélica Maria Nogueira
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  2. Adésio Ferreira
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  3. Marcia Flores da Silva Ferreira
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Correspondence to Marcia Flores da Silva Ferreira.

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Nogueira, A.M., Ferreira, A. & da Silva Ferreira, M.F. Transferability of Microsatellites from Psidium guajava to Eugenia, Myrciaria, Campomanesia, and Syzygium Species (Myrtaceae). Plant Mol Biol Rep 34, 249–256 (2016). https://doi.org/10.1007/s11105-015-0921-7

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