Abstract
Background and aims
Cadmium (Cd) contamination poses a potential threat to plant growth and human health. In this study, we aimed to determine the effect of selenium nanoparticles (SeNPs) on Cd and selenium (Se) uptake and accumulation in bok choy, and investigate the detoxification mechanism of SeNPs on bok choy under Cd stress.
Methods
A pot culture was performed in Cd–contaminated soil with soil applied and foliar–sprayed SeNPs, including SLow, SHigh, FLow, FHigh, and corresponding control treatment. The soil available Cd content, Cd and Se fractions in soil, elements accumulation, subcellular Cd/Se distribution, MDA content, SOD activity, and Fourier transformed infrared spectroscopy (FTIR) were evaluated.
Results
Soil applied SeNPs significantly reduced Cd concentration by 25.9–42.4%, and Cd uptake rate by 33.4–37.8%. Further, soil applied SeNPs had no significant effect on available Cd but did affect Se fractions in soil. Additionally, soil applied SeNPs increased Se concentration by 3.1 − 6.3 times in bok choy and caused a higher Se concentration in root than in shoot, with the residual and organic matter–bound Se mainly affecting Se accumulation in shoot. However, foliar–sprayed SeNPs had no significant effect on Cd uptake but increased Se accumulation by 2.4 − 33.0 times in bok choy. Soil applied and foliar–sprayed SeNPs prompted the distribution of Cd in cell wall and in soluble component in shoot, respectively, which reduced the damage of Cd on organelle.
Conclusion
Soil applied SeNPs was an effective method for reducing Cd accumulation and improving Se biofortification and mineral elements accumulation in bok choy.
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Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
References
Chen D, Chen D, Xue R, Long J, Lin X, Lin Y, Jia L, Zeng R, Song Y (2019) Effects of boron, silicon and their interactions on cadmium accumulation and toxicity in rice plants. J Hazard Mater 367:447–455. https://doi.org/10.1016/j.jhazmat.2018.12.111
Cui J, Liu T, Li Y, Li F (2018) Selenium reduces cadmium uptake into rice suspension cells by regulating the expression of lignin synthesis and cadmium–related genes. Sci Total Environ 644:602–610. https://doi.org/10.1016/j.scitotenv.2018.07.002
Dave R, Tripathi RD, Dwivedi S, Tripathi P, Dixit G, Sharma YK, Trivedi PK, Corpas FJ, Barroso JB, Chakrabarty D (2013) Arsenate and arsenite exposure modulate antioxidants and amino acids in contrasting arsenic accumulating rice (Oryza sativa L.) genotypes. J Hazard Mater 262:1123–1131. https://doi.org/10.1016/j.jhazmat.2012.06.049
Di X, Fu Y, Huang Q, Xu Y, Zheng S, Sun Y (2023a) Comparative effects of copper nanoparticles and copper oxide nanoparticles on physiological characteristics and mineral element accumulation in Brassica chinensis L. Plant Physiol Biochem 196:974–981. https://doi.org/10.1016/j.plaphy.2023.03.002
Di X, Jing R, Qin X, Wei Y, Liang X, Wang L, Xu Y, Sun Y, Huang Q (2023b) Transcriptome analysis reveals the molecular mechanism of different forms of selenium in reducing cadmium uptake and accumulation in wheat seedlings. Chemosphere 340:139888. https://doi.org/10.1016/j.chemosphere.2023.139888
Di X, Qin X, Zhao L, Liang X, Xu Y, Sun Y, Huang Q (2023c) Selenium distribution, translocation and speciation in wheat (Triticum aestivum L.) after foliar–sprayeding selenite and selenate. Food Chem 400:134077. https://doi.org/10.1016/j.foodchem.2022.134077
Dinh QT, Wang M, Tran TAT, Zhou F, Wang D, Zhai H, Peng Q, Xue M, Du Z, Bañuelos GS, Lin ZQ, Liang D (2019) Bioavailability of selenium in soil–plant system and a regulatory approach. Crit Rev Environ Sci Technol 49:443–517. https://doi.org/10.1080/10643389.2018.1550987
Fernández–Martínez A, Charlet L (2009) Selenium environmental cycling and bioavailability: a structural chemist point of view. Rev Environ Sci Bio/Technol 8:81–110. https://doi.org/10.1007/s11157-009-9145-3
Gao J, Liu Y, Huang Y, Lin ZQ, Bañuelos GS, Lam MHW, Yin X (2011) Daily selenium intake in a moderate selenium deficiency area of Suzhou, China. Food Chem 126:1088–1093. https://doi.org/10.1016/j.foodchem.2010.11.137
GB2762-2022 (2022) National standard for food safety-limits of contaminants in food, China
Hart JJ, Welch RM, Norvell WA, Kochian LV (2002) Transport interactions between cadmium and zinc in roots of bread and durum wheat seedlings. Physiol Plant 116:73–78. https://doi.org/10.1034/j.1399-3054.2002.1160109.x
Hasanuzzaman M, Bhuyan MHMB, Raza A, Hawrylak–Nowak B, Matraszek–Gawron R, Mahmud JA, Nahar K, Fujita M (2020) Selenium in plants: Boon or bane? Environ Exp Bot 178:104170. https://doi.org/10.1016/j.envexpbot.2020.104170
Hawrylak–Nowak B, Dresler S, Wójcik M (2014) Selenium affects physiological parameters and phytochelatins accumulation in cucumber (Cucumis sativus L.) plants grown under cadmium exposure. Sci Hort 172:10–18. https://doi.org/10.1016/j.scienta.2014.03.040
He X, Gao J, Hou H, Qi Z, Chen H, Zhang X–X (2019) Inhibition of mitochondrial fatty acid oxidation contributes to development of nonalcoholic fatty liver disease induced by environmental cadmium exposure. Environ Sci Technol 53:13992–14000. https://doi.org/10.1021/acs.est.9b05131
Hu T, Li H, Li J, Zhao G, Wu W, Liu L, Wang Q, Guo Y (2018) Absorption and bio–transformation of selenium nanoparticles by wheat seedlings (Triticum aestivum L). Front Plant Sci 9:597. https://doi.org/10.3389/fpls.2018.00597
Huang BY, Zhao FJ, Wang P (2022a) The relative contributions of root uptake and remobilization to the loading of cd and as into rice grains: implications in simultaneously controlling grain cd and as accumulation using a segmented water management strategy. Environ Pollut 293:118497. https://doi.org/10.1016/j.envpol.2021.118497
Huang Y, Huang B, Shen C, Zhou W, Liao Q, Chen Y, Xin J (2022b) Boron supplying alters cadmium retention in root cell walls and glutathione content in Capsicum annuum. J Hazard Mater 432:128713. https://doi.org/10.1016/j.jhazmat.2022.128713
Hussain B, Lin Q, Hamid Y, Sanaullah M, Di L, Hashmi ML, ur R, Khan MB, He Z, Yang X (2020) Foliage application of selenium and silicon nanoparticles alleviates cd and pb toxicity in rice (Oryza sativa L). Sci Total Environ 712:136497. https://doi.org/10.1016/j.scitotenv.2020.136497
Ismael MA, Elyamine AM, Moussa MG, Cai M, Zhao X, Hu C (2019) Cadmium in plants: uptake, toxicity, and its interactions with selenium fertilizers. Metallomics 11:255–277. https://doi.org/10.1039/C8MT00247A
Jayalath S, Wu H, Larsen SC, Grassian VH (2018) Surface adsorption of Suwannee river humic acid on TiO2 nanoparticles: a study of pH and particle size. Langmuir 34:3136–3145. https://doi.org/10.1021/acs.langmuir.8b00300
Kang L, Wu Y, Zhang J, An Q, Zhou C, Li D, Pan C (2022) Nano–selenium enhances the antioxidant capacity, organic acids and cucurbitacin B in melon (Cucumis melo L.) plants. Ecotoxicol Environ Saf 241:113777. https://doi.org/10.1016/j.ecoenv.2022.113777
Krishnamurti GSR, Naidu R (2002) Solid – solution speciation and phytoavailability of copper and zinc in soils. Environ Sci Technol 36:2645–2651. https://doi.org/10.1021/es001601t
Kumar A, Prasad KS (2020) Role of nano-selenium in health and environment. J Biotechnol 325:152–163. https://doi.org/10.1016/j.jbiotec.2020.11.004
Lee J, Park I, Lee ZW, Kim SW, Baek N, Park HS, Park SU, Kwon S, Kim H (2013) Regulation of the major vacuolar Ca2+ transporter genes, by intercellular Ca2+ concentration and abiotic stresses, in tip–burn resistant Brassica oleracea. Mol Biol Rep 40:177–188. https://doi.org/10.1007/s11033-012-2047-4
Li Y, Zhu N, Liang X, Zheng L, Zhang C, Li YF, Zhang Z, Gao Y, Zhao J (2020) A comparative study on the accumulation, translocation and transformation of selenite, selenate, and SeNPs in a hydroponic–plant system. Ecotoxicol Environ Saf 189:109955. https://doi.org/10.1016/j.ecoenv.2019.109955
Li D, Zhou C, Wu Y, An Q, Zhang J, Fang Y, Li JQ, Pan C (2022) Nanoselenium integrates soil–pepper plant homeostasis by recruiting rhizosphere–beneficial microbiomes and allocating signaling molecule levels under cd stress. J Hazard Mater 432:128763. https://doi.org/10.1016/j.jhazmat.2022.128763
Lu RK (2000) The analysis method of soil agricultural chemistry. Chinese Agricultural Science Press, Beijing (in Chinese)
Lyu L, Wang H, Liu R, Xing W, Li J, Man YB, Wu F (2022) Size–dependent transformation, uptake, and transportation of SeNPs in a wheat–soil system. J Hazard Mater 424:127323. https://doi.org/10.1016/j.jhazmat.2021.127323
Morales-Espinoza MC, Cadenas-Pliego G, Perez-Alvarez M, Hernandez-Fuentes AD, Cabrera de la Fuente M, Benavides-Mendoza A, Valdes-Reyna J, Juarez-Maldonado A (2019) Se nanoparticles induce changes in the growth, antioxidant responses, and fruit quality of tomato developed under NaCl stress. Molecules 24. https://doi.org/10.3390/molecules24173030
Poblaciones MJ, Broadley MR (2022) Foliar selenium biofortification of broccolini: effects on plant growth and mineral accumulation. J Hortic Sci Biotechnol 97:730–738. https://doi.org/10.1080/14620316.2022.2068458
Riaz M, Kamran M, Rizwan M, Ali S, Parveen A, Malik Z, Wang X (2021) Cadmium uptake and translocation: selenium and silicon roles in cd detoxification for the production of low cd crops: a critical review. Chemosphere 273:129690. https://doi.org/10.1016/j.chemosphere.2021.129690
Rochfort SJ, Imsic M, Rod J, Craige TV, Tomkins B (2006) Characterization of flavonol conjugates in immature leaves of pak choi [Brassica rapa L. Ssp. chinensis L. (Hanelt.)] by HPLC–DAD and LC–MS/MS. J Agric Food Chem 53:4855–4860. https://doi.org/10.1021/JF060154J
Sardar R, Ahmed S, Shah AA, Yasin NA (2022) Selenium nanoparticles reduced cadmium uptake, regulated nutritional homeostasis and antioxidative system in Coriandrum sativum grown in cadmium toxic conditions. Chemosphere 287:132332. https://doi.org/10.1016/j.chemosphere.2021.132332
Treesubsuntorn C, Thiravetyan P (2019) Calcium acetate-induced reduction of cadmium accumulation in Oryza sativa: expression of auto–inhibited calcium–ATP ase and cadmium transporters. Plant Biol J 21:862–872. https://doi.org/10.1111/plb.12990
Trippe RC, Pilon–Smits EAH (2021) Selenium transport and metabolism in plants: phytoremediation and biofortification implications. J Hazard Mater 404:124178. https://doi.org/10.1016/j.jhazmat.2020.124178
Wang S, Liang D, Wang D, Wei W, Fu D, Lin Z (2012) Selenium fractionation and speciation in agriculture soils and accumulation in corn (Zea mays L.) under field conditions in Shaanxi Province, China. Sci Total Environ 427–428:159–164. https://doi.org/10.1016/j.scitotenv.2012.03.091
Wang C, Ji J, Zhu F (2017) Characterizing Se transfer in the soil-crop systems under field condition. Plant Soil 415:535–548. https://doi.org/10.1007/s11104-017-3185-1
Wang H, Chen W, Sinumvayabo N, Li Y, Han Z, Tian J, Ma Q, Pan Z, Geng Z, Yang S, Kang M, Rahman SU, Yang G, Zhang Y (2020a) Phosphorus deficiency induces root proliferation and cd absorption but inhibits cd tolerance and cd translocation in roots of Populus × euramericana. Ecotoxicol Environ Saf 204:111148. https://doi.org/10.1016/j.ecoenv.2020.111148
Wang K, Wang Y, Li K, Wan Y, Wang Q, Zhuang Z, Guo Y, Li H (2020b) Uptake, translocation and biotransformation of selenium nanoparticles in rice seedlings (Oryza sativa L). J Nanobiotechnol 18:103. https://doi.org/10.1186/s12951-020-00659-6
Wang Y, Xu Y, Liang X, Wang L, Sun Y, Huang Q, Qin X, Zhao L (2021) Soil application of manganese sulfate could reduce wheat cd accumulation in cd contaminated soil by the modulation of the key tissues and ionomic of wheat. Sci Total Environ 770:145328. https://doi.org/10.1016/j.scitotenv.2021.145328
Wang L, Wu K, Liu Z, Li Z, Shen J, Wu Z, Liu H, You L, Yang G, Rensing C, Feng R (2023) Selenite reduced uptake/translocation of cadmium via regulation of assembles and interactions of pectins, hemicelluloses, lignins, callose and casparian strips in rice roots. J Hazard Mater 448:130812. https://doi.org/10.1016/j.jhazmat.2023.130812
White PJ (2018) Selenium metabolism in plants. Biochim Biophys Acta (BBA) – Gen Subj 1862:2333–2342. https://doi.org/10.1016/j.bbagen.2018.05.006
Yadav M, Gupta P, Seth CS (2022) Foliar application of α–lipoic acid attenuates cadmium toxicity on photosynthetic pigments and nitrogen metabolism in Solanum lycopersicum L. Acta Physiol Plant 44:112. https://doi.org/10.1007/s11738-022-03445-z
Yu Y, Wang Q, Wan Y, Huang Q, Li H (2023) Transcriptome analysis reveals different mechanisms of selenite and selenate regulation of cadmium translocation in Brassica rapa. J Hazard Mater 452:131218. https://doi.org/10.1016/j.jhazmat.2023.131218
Yuan Y, Imtiaz M, Rizwan M, Dai Z, Hossain MM, Zhang Y, Huang H, Tu S (2022) The role and its transcriptome mechanisms of cell wall polysaccharides in vanadium detoxication of rice. J Hazard Mater 425:127966. https://doi.org/10.1016/j.jhazmat.2021.127966
Zhao Y, Hu C, Wang X, Qing X, Wang P, Zhang Y, Zhang X, Zhao X (2019) Selenium alleviated chromium stress in chinese cabbage (Brassica campestris L. ssp. Pekinensis) by regulating root morphology and metal element uptake. Ecotoxicol Environ Saf 173:314–321. https://doi.org/10.1016/j.ecoenv.2019.01.090
Zhou J, Zhang C, Du B, Cui H, Fan X, Zhou D, Zhou J (2021) Soil and foliar applications of silicon and selenium effects on cadmium accumulation and plant growth by modulation of antioxidant system and cd translocation: comparison of soft vs. durum wheat varieties. J Hazard Mater 402:123546. https://doi.org/10.1016/j.jhazmat.2020.123546
Zhu J, Zhao P, Nie Z, Shi H, Li C, Wang Y, Qin S, Qin X, Liu H (2020) 1Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum). BMC Plant Biol 20:550. https://doi.org/10.1186/s12870-020-02763-z
Zhu Y, Dong Y, Zhu N, Jin H (2022) Foliar application of biosynthetic nano–selenium alleviates the toxicity of cd, pb, and hg in Brassica chinensis by inhibiting heavy metal adsorption and improving antioxidant system in plant. Ecotoxicol Environ Saf 240:113681. https://doi.org/10.1016/j.ecoenv.2022.113681
Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (No. 41601343).
Funding
This work was financially supported by the National Natural Science Foundation of China (No. 41601343).
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Xuerong Di: Conceptualization, Validation, Writing – original draft; Qingqing Huang: Data curation, Writing – original draft; Writing – review & editing; Funding acquisition, Supervision; Rui Jing: Writing – review & editing, Formal analysis; Yuebing Sun: Writing – original draft, Writing – review & editing, Supervision.
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Di, X., Jing, R., Xie, S. et al. Biofortification of bok choy with selenium nanoparticles and its inhibitory effects on cadmium accumulation. Plant Soil 494, 701–716 (2024). https://doi.org/10.1007/s11104-023-06318-7
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DOI: https://doi.org/10.1007/s11104-023-06318-7