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Physiological, genomic and transcriptomic comparison of two Brassica napus cultivars with contrasting cadmium tolerance

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Abstract

Aims

Cadmium (Cd) is the most widespread toxic heavy metal to plant growth. As the second leading oil crop, some genotypes of Brassica napus (B. napus) are potential Cd accumulators. However, the Cd translocation mechanism from root to shoot in B. napus in response to Cd toxicity remains unknown.

Methods

In the present study, a couple of B. napus genotypes with contrasting Cd uptake and root-to-shoot translocation abilities, named P78 (the high Cd accumulator, HC) and P72 (the low Cd accumulator, LC), were chosen from 39 B. napus genotypes with various Cd accumulation features.

Results

Physiological comparison of P78 and P72 reveals that P72 is more sensitive to Cd toxicity than P78. With genomic resequencing, transcriptomics and qRT-PCR assay, BnNramp2;1 and BnNramp4;2 were focused with highly upregulation in shoot of P78 under Cd treatment condition. Furthermore, BnNramp2;1 and BnNramp4;2 can successfully complement the function of tonoplast localized Cd transporter YCF1. And when BnNramp2;1 and BnNramp4;2 were transferred in Arabidopsis atnramp mutants, the transgenic plants showed better growth rate than mutants under higher Cd stress conditions.

Conclusions

The results reveals that BnNramp2;1 and BnNramp4;2 were two main Cd transporters associated with enhanced root-to-shoot translocation and accumulation of Cd in shoot of B. napus.

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Abbreviations

APX:

Ascorbate peroxidase

AsA:

Ascorbic acid

BCF:

Biological enrichment factor

B.napus :

Brassica napus

Ca:

Calcium

CAT:

Catalase

Cd:

Cadmium

Ci:

Intercellular CO2 concentration

Cu:

Copper

DEGs:

Differentially expressed genes

Fe:

iron

GO:

Gene ontology

GR:

Glutathione reductase

Gs:

Stomatal conductance

GSH:

Glutataione

HC:

High Cd accumulator

LC:

Low Cd accumulator

MDA:

Malondialdehyde

MDHAR:

Monodehydroascorbate reductase

Mg:

Magnesium

Mn:

Manganese

Pn:

Variation of net CO2 assimilation

POD:

Peroxidase

R:

Root

S:

Shoot

SOD:

Superoxide dismutase

TF:

Translocation factor

Tr:

Transpiration

Zn:

Zinc

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Acknowledgements

This work was supported by National Key R & D Program of China (2018YFD0800600, 2018YFD0200903), National Natural Science Foundation of China (31870587; 31400063; 31500038) and Fundamental Research Funds for the Central Universities (XDJK2017B030; SWU116021; XDJK2018C095; SWU118114; SWU115018), Research Funds of Scientific Platform and Base Construction (cstc2014pt-sy0017), and The Recruitment Program for Foreign Experts (No. WQ20125500073).

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Authors and Affiliations

  1. Chongqing Key Lab of Bioresource for Energy, College of Resources and Environment, Southwest University, Beibei, Chongqing, 400715, People’s Republic of China

    Shufeng Wang, Juanjuan Sun, Shengting Li, Hongjun Meng, Zhen Zhang, Feng Luo & Nannan Li

  2. Academy of Agricultural Science, Southwest University, Beibei, Chongqing, 400715, People’s Republic of China

    Kun Lu, Zhongchun Xiao, Jiana Li & Nannan Li

  3. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, People’s Republic of China

    Kun Lu, Zhongchun Xiao & Jiana Li

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  1. Shufeng Wang
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  2. Juanjuan Sun
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Contributions

SW and NL designed and conceived the study and drafted the manuscript. SW, JS, KL and SL performed experiments and data analysis. NL, SW, KL, HM, ZX, JL, FL and SL coordinated the research and helped to finalize the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Feng Luo or Nannan Li.

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Responsible Editor: Juan Barcelo.

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Physiological, genomic and transcriptomic comparison of two rapeseed genotypes and functional characterization reveals that two BnNramps play important role in resistance to Cadmium toxicity

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Wang, S., Sun, J., Li, S. et al. Physiological, genomic and transcriptomic comparison of two Brassica napus cultivars with contrasting cadmium tolerance. Plant Soil 441, 71–87 (2019). https://doi.org/10.1007/s11104-019-04083-0

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