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Growth, V uptake, and antioxidant enzymes responses of chickpea (Cicer arietinum L.) genotypes under vanadium stress

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Abstract

Aims

Heavy metals pollution is one of the most challenging problems to the environment and agricultural soils in recent decades. The purpose of the present work was to elucidate the effects of vanadium (V) on growth, V uptake, protein content and enzymes activity to sort out the V-tolerant and the sensitive genotypes of chickpea under hydroponic conditions.

Methods

The activities of antioxidant enzymes (SOD, CAT, POD, and GSH, MDA) and protein contents were determined by using UV-1600 Spectrophotometer, and V concentration was determined by using GFAAS (GTA 120).

Results

The findings show that V significantly increased the enzymes activities in all chickpea genotypes however, V significantly reduced the protein contents, and more accumulation of V was observed in roots than shoots in all genotypes. The plant biomass and lengths of roots and shoots were also significantly reduced by V. Moreover, NH4VO3 caused more toxicity than Na3VO4.

Conclusions

The previous studies report that higher activities of enzymes increase the tolerance of plants against stress. The obtained data of present study indicated that Noor–2009 and C–44 are tolerant and G–1 and Balkasar are sensitive genotypes of chickpea against V stress.

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References

  • Aebi HE, Bergmeyer HO (1983) Catalase, methods enzymology. Academic, New York, p 2

    Google Scholar 

  • Alan O, Gesch FF, David A, Jana R (2005) Importance of vanadium and nutrient ionic ratios on the development of hydroponically grown cuphea. Ind Crop Prod 21:165–171

    Article  Google Scholar 

  • Amorim FAC, Welz B, Costa ACS, Lepri FG, Vale MGRV, Ferreira SLC (2007) Determination of vanadium in petroleum and petroleum products using atomic spectrometric techniques. Talanta 72:349–359

    Article  CAS  PubMed  Google Scholar 

  • Andon V, Fernando L (2011) Cd-induced membrane damages and changes in soluble protein and free amino acid contents in young barley plants. Emir J Food Agric 23(2):130–136

    Google Scholar 

  • Anke M (2004) Vanadium an element both essential and toxic to plants, animals and humans? Anales de la Real Acad Nacional de Farmacia Madrid 70(4):961–999

    CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  PubMed  Google Scholar 

  • Boulassel B, Sadeg N, Roussel O, Perrin M, Belhadj-Tahar H (2011) Fatal poisoning by vanadium. Forensic Sci Int 206(1):79–81

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizating the principle of protein dyes binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Cam MC, Brownsey RW, McNeill JH (2000) Mechanisms of vanadium action: insulin mimetic or insulin-enhancing agent? Can J Physiol Pharm 78:829–847

    Article  CAS  Google Scholar 

  • Chary NS, Kamala CT, Raj DS (2008) Assessing risk of heavy metals from consuming food grown on sewage irrigated soils and food chain transfer. Ecotoxicol Environ Saf 69:513–524

    Article  CAS  PubMed  Google Scholar 

  • Clemens S (2006) Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie 88:1707–1719

    Article  CAS  PubMed  Google Scholar 

  • Dazy M, Masfaraud JF, Ferard JF (2009) Induction of oxidative stress biomarkers associated with heavy metal stress in Fontinalis antipyretica Hedw. Chemosphere 75:297–302

    Article  CAS  PubMed  Google Scholar 

  • Guo TR, Zhang GP, Zhou MX, Wu FB, Chen JX (2004) Effect of aluminum and cadmium toxicity on growth and antioxidant enzyme activity of two barley genotypes with different aluminum tolerance. Plant Soil 258:241–248

    Article  CAS  Google Scholar 

  • Gupta DK, Nicoloso FT, Schetinger MRC, Rossato LV, Pereira LB, Castro GY, Srivastava S, Tripathii RD (2009) Antioxidant defence mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. J Hazard Mater 172(1):479–484

    Article  CAS  PubMed  Google Scholar 

  • Hassan M, Mansoor S (2014) Oxidative stress and antioxidant defense mechanism in mung bean seedlings after lead and cadmium treatments. Turk J Agric For 38:55–61

    Article  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts I. Kinetic and stoichiometery of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  PubMed  Google Scholar 

  • Hemalatha S, Anburaj A, Francis K (1997) Effect of heavy metal s on certain biochemical constituent s and nitrate reductase activity in Orzya sativa L. Seedl ings. J Environ Biol 18:313–319

    CAS  Google Scholar 

  • Hou W, Chen X, Song G, Wang Q, Chang CC (2007) Effects of copper and cadmium on heavy metal polluted waterbody restoration by duckweed (Lemna minor). Plant Physiol Biochem 45:62–69

    Article  CAS  PubMed  Google Scholar 

  • Jain R, Srivastava S, Solomon S, Shrivastava AK, Chandra A (2010) Impact of excess zinc on growth parameters, cell division, nutrient accumulation, photosynthetic pigments and oxidative stress of sugarcane (Saccharum spp.). Acta Physiol Plant 32:979–986

    Article  CAS  Google Scholar 

  • Kar BB, Datta P, Misra VN (2004) Spent catalyst: secondary source for molybdenum recovery. Hydrometall 72:87–92

    Article  CAS  Google Scholar 

  • Kashif SR, Akram M, Yaseen M, Ali S (2009) Studies on heavy metals status and their uptake by vegetables in adjoining areas of Hudiara drain in Lahore. Soil Environ 28(1):7–12

    CAS  Google Scholar 

  • Kaur G, Harminder PS, Daizy RB, Ravinder KK (2012) Growth, photosynthetic activity and oxidative stress in wheat (Triticum aestivum) after exposure of lead to soil. J Environ Biol 33:265–269

    CAS  PubMed  Google Scholar 

  • Kaya C, Tuna L, Higgs D (2006) Effect of silicon on plant growth and mineral nutrition of maize grown under water stress conditions. J Plant Nut 29:1469–1480

    Article  CAS  Google Scholar 

  • Ke WS, Xiong ZT, Xie MJ, Luo Q (2007) Accumulation, subcellular localization and ecophysiological responses to copper stress. Plant Soil 292:291–304

    Article  CAS  Google Scholar 

  • Kraepiel AM, Bellenger JP, Wichard T, Morel FM (2009) Multiple roles of siderophores in free-living nitrogen-fixing bacteria. Biometals 22:573–581

    Article  CAS  PubMed  Google Scholar 

  • Lagriffoul A, Mocquot B, Mench M, Vangronsveld J (1998) Cadmium toxicity effects on growth, mineral and chlorophyll contents, and activities of stress related enzymes in young maize plants (Zea mays L.). Plant Soil 200:241–250

    Article  CAS  Google Scholar 

  • Li HS (2000) Principles and techniques of plant physiological biochemical experiment. Higher Education Press, Beijing, in Chinese

    Google Scholar 

  • Liang YC, Sun WC, Zhu YG, Christie P (2007) Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: a review. Environ Pollut 147:422–428

    Article  CAS  PubMed  Google Scholar 

  • Lozano R, Azcon R, Palma JM (1996) SOD and drought stress in Lactua sativa. New Phytol 136:329–331

    Google Scholar 

  • Mendoza-Cózatl DG, Moreno-Sánchez R (2006) Control of glutathione and phytochelatin synthesis under cadmium stress. Pathway modeling for plants. J Theor Biol 238:919–936

    Article  PubMed  Google Scholar 

  • Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud 15:523–530

    CAS  Google Scholar 

  • Myrene R, D’Souza Devaraj VR (2012) Induction of oxidative stress and antioxidative mechanisms in hyacinth bean under zinc stress. Afr Crop Sci J 20(1):17–29

    Google Scholar 

  • Narumol V, Yaowapha J, Greg D, Ashley TT, Paul RH (2011) Effect of vanadium on plant growth and its accumulation in plant tissues. Songklanakarin J Sci Technol 33(3):255–261

    Google Scholar 

  • Olness A, Archer DW, Gesch RW, Rinke J (2002) Resin extractable phosphorus, vanadium, calcium and magnesium as factors in maize (Zea mays L.) yield. J Agron Crop Sci 188:94–101

    Article  CAS  Google Scholar 

  • Pereira GJG, Molina SMG, Lea PJ, Azevedo RA (2002) Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea. Plant Soil 239:123–132

    Article  CAS  Google Scholar 

  • Rascio N, Della-Vecchia F, La-Rocca N, Barbato R, Pagliano C, Raviolo M, Gonnelli C, Gabbrielli R (2008) Metal accumulation and damage in rice (cv. Vialone nano) seedlings exposed to cadmium. Environ Exp Bot 62:267–278

    Article  CAS  Google Scholar 

  • Rosso PH, Pushnik JC, Lay M, Ustin SL (2005) Reflectance properties and physiological responses of Salicornia virginicato heavy metal and petroleum contamination. Environ Pollut 137:241–252

    Article  CAS  PubMed  Google Scholar 

  • Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:1192–1205

    Google Scholar 

  • Tanyolaç D, Ekmekçi Y, Ünalan Æ (2007) Changes in photochemical and antioxidant enzyme activities in maize (Zea mays L.) leaves exposed to excess copper. Chemosphere 67:89–98

    Article  PubMed  Google Scholar 

  • Thompson KH, Lichter J, LeBel C, Scaife MC, McNeill JH, Orvig C (2009) Vanadium treatment of type 2 diabetes: a view to the future. J Inorg Biochem 103:554–558

    Article  CAS  PubMed  Google Scholar 

  • Vachirapatama N, Jirakiattikul Y, Dicinoski G, Townsend AT, Haadad PR (2011) Effect of vanadium on plant growth and its accumulation in plant tissues. Songklanakarin J Sci Technol 33(3):255–261

    CAS  Google Scholar 

  • Wahid A, Ghani A (2008) Varietal differences in mungbean (Vigna radiata) for growth, yield, toxicity symptoms and cadmium accumulation. Ann Appl Biol 152:59–69

    Article  CAS  Google Scholar 

  • Wang JF, Liu Z (1999) Effect of vanadium on the growth of soybean seedlings. Plant Soil 216(1–2):47–51

    Article  CAS  Google Scholar 

  • Wani PA, Khan MS, Zaidi A (2005) An evaluation of the effects of heavy metals on the growth, seed yield and grain protein of lentil in pots. Ann Appl Biol 27:23–24

    Google Scholar 

  • Xiao S, Li YY, Quan FZ, Wei BW (2012) Effect of Pb toxicity on leaf growth, antioxidant enzyme activities, and photosynthesis in cuttings and seedlings of Jatropha curcas L. Environ Sci Pollut Res 19:893–902

    Article  Google Scholar 

  • Xi-yuan X, Miao Y, Zhao-hui G, Yue-ping L, Jun-ping B (2012) Permissible value for vanadium in allitic udic ferrisols based on physiological responses of green Chinese cabbage and soil microbes. Biol Trace Elem Res 145:225–232

    Article  Google Scholar 

  • Zhang S, Zhang H, Qin R, Jiang W, Liu D (2009) Cadmium induction of lipid per oxidation and effects on root tip cells and antioxidant enzyme activities in Vicia faba L. Ecotoxicol 18:814–823

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported in part by the National Science Foundation (41071309), Research grants from Sino Hydropower Group (GW-KJ-2012-10-01), and Special Fund for Agro-scientific Research in the Public Interest (201303106, 201103007). The authors gratefully acknowledge Sha Le Le, Li Mao, Yizhu Liu to help during study and laboratory work.

Conflict of Interest

None of the authors have any conflict of interest.

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Authors and Affiliations

  1. Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China

    Muhammad Imtiaz, Shuxin Tu, Zhijian Xie, Dan Han & Muhammad Shahid Rizwan

  2. Microelement Research Center, Huazhong Agricultural University, Wuhan, 430070, China

    Muhammad Imtiaz, Shuxin Tu, Zhijian Xie, Dan Han & Muhammad Shahid Rizwan

  3. Department of Soil and Environmental Sciences, University College of Agriculture, University of Sargodha, Sargodha, 40100, Pakistan

    Muhammad Ashraf

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  1. Muhammad Imtiaz
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  2. Shuxin Tu
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  3. Zhijian Xie
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Correspondence to Shuxin Tu.

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Responsible Editor: Henk Schat.

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Imtiaz, M., Tu, S., Xie, Z. et al. Growth, V uptake, and antioxidant enzymes responses of chickpea (Cicer arietinum L.) genotypes under vanadium stress. Plant Soil 390, 17–27 (2015). https://doi.org/10.1007/s11104-014-2341-0

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  • DOI: https://doi.org/10.1007/s11104-014-2341-0

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