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Isothiocyanates inhibit fungal pathogens of potato in in vitro assays

Isothiocyanates produced by Brassica spp. inhibit growth of three economically important potato pathogens

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Abstract

Aims

This study aimed to examine the effects of seven different isothiocyanates against the growth and development of three important soil borne potato pathogens, (Colletotrichum coccodes, Rhizoctonia solani and Helminthosporium solani).

Methods

The study was carried out using an agar diffusion assay. The radial growth of fungal pathogens grown on agar containing different ITCs at a range of concentrations was compared to that of growth on control agar plates that did not contain ITCs.

Results

Results varied depending on the specific isothiocyanate incorporated into the agar. They ranged from those which showed a significant effect on fungal growth to those which appeared to have little or no effect. Where a suppressive effect was observed, due to the presence of the isothiocyanate, fungal colony growth decreased as the concentration of the incorporated isothiocyanate increased.

Conclusions

Results from this study indicate that fungal growth can be inhibited by exposure to ITCs. However the results observed are specific to the ITC structure and exposure concentration.

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Acknowledgments

The authors acknowledge funding from the John Oldacre Foundation and Barworth Agriculture Ltd and Potato Section, SASA for providing fungal cultures.

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Authors and Affiliations

  1. Science and Advice for Scottish Agriculture (SASA), Roddinglaw Road, Edinburgh, EH12 9FJ, UK

    Fiona Isabelle Taylor & David Kenyon

  2. University of Glasgow, University Avenue, Glasgow, G12 8QQ, UK

    Fiona Isabelle Taylor & Susan Rosser

Authors
  1. Fiona Isabelle Taylor
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  2. David Kenyon
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  3. Susan Rosser
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Correspondence to Fiona Isabelle Taylor.

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Responsible Editor: John A. Kirkegaard.

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Taylor, F.I., Kenyon, D. & Rosser, S. Isothiocyanates inhibit fungal pathogens of potato in in vitro assays. Plant Soil 382, 281–289 (2014). https://doi.org/10.1007/s11104-014-2157-y

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