Abstract
Key message
A novel gene NbGIS positively regulates glandular trichome initiation through GA Signaling in tobacco. NbMYB123-like regulates glandular trichome initiation by acting downstream of NbGIS in tobacco.
Abstract
Glandular trichome is a specialized multicellular structure which has capability to synthesize and secrete secondary metabolites and protects plants from biotic and abiotic stresses. Our previous results revealed that a C2H2 zinc-finger transcription factor GIS and its sub-family genes act upstream of GL3/EGL3-GL1-TTG1 transcriptional activator complex to regulate trichome initiation in Arabidopsis. In this present study, we found that NbGIS could positively regulate glandular trichome development in Nicotiana benthamiana (tobacco). Our result demonstrated that 35S:NbGIS lines exhibited much higher densities of trichome on leaves, main stems, lateral branches and sepals than WT plants, while NbGIS:RNAi lines had the opposite phenotypes. Furthermore, our results also showed that NbGIS was required in response to GA signal to control glandular trichome initiation in Nicotiana benthamiana. In addition, our results also showed that NbGIS significantly influenced GA accumulation and expressions of marker genes of the GA biosynthesis, might result in the changes of growth and maturation in tobacco. Lastly, our results also showed that NbMYB123-like regulated glandular trichome initiation in tobacco by acting downstream of NbGIS. These findings provide new insights to discover the molecular mechanism by which C2H2 transcriptional factors regulates glandular trichome initiation through GA signaling pathway in tobacco.
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Abbreviations
- bHLH:
-
Basic helix-loop-helix
- CKs:
-
Cytokinins
- Ct:
-
Cycle threshold
- GA:
-
Gibberellin acid
- GIS:
-
Glabrous inflorescence stems
- JAs:
-
Jasmonates
- SA:
-
Salicylic acid
- VIGS:
-
Virus-induced gene silencing
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Acknowledgements
The research was funded by National Natural Science Foundation of China (Grant Nos. 31529001; 31570183; 31661143004); Zhejiang Provincial Natural Science Foundation of China (Grant No. LZ15C020001); Major State Basic Research Development Program (973 Program, Grant No. 2015CB150200) and the National Key R & D Program of China (2016YFD0100701).
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YL contributed to all experiments and manuscript preparation. YG, YH and YL conceived and designed the study and wrote the manuscript; DL and BL analyzed data; YL, JW and GS involved in gene cloning, gene transformation and hormone experiments; MW, ARK, HY, HN and LH contributed to gene expression analysis and trichome phenotype analyses. All authors read and approved the final manuscript.
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Liu, Y., Liu, D., Khan, A.R. et al. NbGIS regulates glandular trichome initiation through GA signaling in tobacco. Plant Mol Biol 98, 153–167 (2018). https://doi.org/10.1007/s11103-018-0772-3
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DOI: https://doi.org/10.1007/s11103-018-0772-3