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Ultrasonic-Combined Plasma Bubbling for Adherent Bacteria Disinfection on Medical Equipment

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Abstract

Atmospheric low-temperature plasma has received attention for application in disinfection methods. In this study, we develop a plasma bubbling method as a disinfection method. In the plasma bubbling method, the bactericidal effect can be introduced into the water by bubbling with carbon dioxide plasma. The plasma bubbling method is effective for the treatment of Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Serratia marcescens, and Fusarium solani, which pose problems in the field of ophthalmology. In addition, we develop ultrasonic-combined plasma bubbling, in which plasma bubbling imposes a bactericidal effect, whereas ultrasonic exerts a detachment effect as a disinfection method for adherent bacteria on medical equipment. Ultrasonic-combined plasma bubbling demonstrates a prominent bactericidal effect on P. aeruginosa adherent bacteria on stainless steel plates and can be used to the measuring prism (MP) of applanation tonometers. In observation the MP treated with plasma bubbling and ultrasonic-combined plasma bubbling for 11 h, no damage to the MPs was observed.

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Acknowledgements

This study was based on the Cooperative Research Project of the Research Center for Biomedical Engineering and was supported by JSPS KAKENHI Grant Number [20K07039]. This research is based on the Cooperative Research Project of Research Center for Biomedical Engineering.

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Authors and Affiliations

  1. Laboratory for Future Interdisciplinary Research of Science and Technology, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8502, Japan

    Yuma Suenaga, Hiroaki Kawano & Akitoshi Okino

  2. Research Institute for Biomedical Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba, 278-0022, Japan

    Toshihiro Takamatsu

  3. Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, 6-5-1 Kashiwanoha, Kashiwa, Chiba, 277-8577, Japan

    Toshihiro Takamatsu

  4. Division of Infection Prevention and Control, Tokyo Healthcare University, 4-1-17 Higashi-Gotanda, Shinagawa-ku, Tokyo, 141-8648, Japan

    Yuriko Matsumura & Atsuo Iwasawa

  5. Veterinary Medical Center, Tottori University, 4-101 Minami, Koyama-cho, Tottori, 680-8550, Japan

    Norihiko Ito

Authors
  1. Yuma Suenaga
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  2. Hiroaki Kawano
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  3. Toshihiro Takamatsu
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  4. Yuriko Matsumura
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  5. Norihiko Ito
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  6. Atsuo Iwasawa
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  7. Akitoshi Okino
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Contributions

All authors contributed to the study conception and design. Productions of Plasma bubbling device were performed by AO, YS, HK. Material preparations were performed by NI. Developments of methodology were performed by YM, NI, AI. Data collections and analyses were performed by YS, HK, TT, YM, NI, AI, AO. The first draft of the manuscript was written by YS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Akitoshi Okino.

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Suenaga, Y., Kawano, H., Takamatsu, T. et al. Ultrasonic-Combined Plasma Bubbling for Adherent Bacteria Disinfection on Medical Equipment. Plasma Chem Plasma Process 42, 575–586 (2022). https://doi.org/10.1007/s11090-022-10241-7

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  • DOI: https://doi.org/10.1007/s11090-022-10241-7

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